Clinical and Experimental Reproductive Medicine

  • 제39권2호
  • /
  • Pages.81-86
  • /
  • 2012
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지
DOI QR코드

DOI QR Code

Prooxidant-antioxidant balance and malondialdehyde over time in adult rats after tubal sterilization and vasectomy

  • Faramarzi, Azita (Department of Physiology, School of Medicine, Tehran University of Medical Science) ;
  • Seifi, Behjat (Department of Physiology, School of Medicine, Tehran University of Medical Science) ;
  • Sadeghipour, Hamid Reza (Department of Physiology, School of Medicine, Tehran University of Medical Science) ;
  • Shabanzadeh, Alireza (Department of Physiology, School of Medicine, Tehran University of Medical Science) ;
  • Ebrahimpoor, Mitra (Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Science)
  • 투고 : 2012.03.26
  • 심사 : 2012.05.03
  • 발행 : 2012.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: Sterilization (tubal sterilization and vasectomy) is a widely applied contraceptive method worldwide. Although most studies have described sterilization as a safe method, there are reports of tubal ligation (TL) and vasectomy complications. The aim of this study was to evaluate the effects of TL and vasectomy on the serum oxidative stress, specifically prooxidant-antioxidant balance (PAB) and malondialdehyde (MDA) levels, over time. Methods: Male and female rats were classified into vasectomy, sham-vasectomy, TL, and sham-TL groups, respectively. The PAB and MDA levels were measured on days 15 and 45 and months 3 and 6 after the intervention. For female rats, blood sampling was performed during the diestrous phase and estradiol and progesterone were also measured. Results: Serum PAB and MDA increased after TL (p<0.05). Vasectomy increased serum MDA remarkably after 45 days, 3 months, and 6 months (p<0.05). After vasectomy, serum PAB also increased although not significantly. Serum estradiol and progesterone decreased remarkably in the TL group compared to the sham group (p<0.05). Conclusion: Bilateral TL and vasectomy both increase the serum oxidative stress; however the imbalance after TL was very noticeable. As for the TL, the reduction of serum estrogen levels can be involved in this imbalance. Complications followed by TL or vasectomy could be due to increased levels of oxidants. Thus, prescribing antioxidants during and or after surgery may be a solution.

키워드

과제정보

연구 과제 주관 기관 : Tehran University of Medical Science

참고문헌

  1. Peterson HB. Sterilization. Obstet Gynecol 2008;111:189-203.
  2. Aygen EM, Ozdamar S, Serin S, Babug M. Ovarian morphology of rats after fallopian tube sterilization. Contraception 2002;66:211-4.
  3. Ozerkan K, Aydin G, Koc I, Uncu Y, Uncu G. Menstrual pattern following tubal sterilization. Med Sci Monit 2010;16:CR197-201.
  4. Mall A, Shirk G, Van Voorhis BJ. Previous tubal ligation is a risk factor for hysterectomy after rollerball endometrial ablation. Obstet Gynecol 2002;100:659-64.
  5. Wyshak G. Tubal ligation and the risk of vertebral fractures. Osteoporos Int 2005;16:651-8.
  6. Kutlar I, Ozkur A, Balat O, Ugur MG, Genco Y, Aksoy F. Effects of three different sterilization methods on utero-ovarian Doppler blood flow and serum levels of ovarian hormones. Eur J Obstet Gynecol Reprod Biol 2005;122:112-7.
  7. Gentile GP, Kaufman SC, Helbig DW. Is there any evidence for a post-tubal sterilization syndrome? Fertil Steril 1998;69:179-86.
  8. Kilic S, Tasdemir N, Lortlar N, Yuksel B, Budak G, Batioglu S. Vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) immunoreactivities in rat ovaries and uterine tubes after tubal ligation: a controlled immunohistochemical study. Eur J Contracept Reprod Health Care 2008;13:431-7.
  9. Lavers AE, Swanlund DJ, Hunter BA, Tran ML, Pryor JL, Roberts KP. Acute effect of vasectomy on the function of the rat epididymal epithelium and vas deferens. J Androl 2006;27:826-36.
  10. Melville C, Bigrigg A. Male and female sterilization. Obstet Gynecol Reprod Med 2008;18:330-4.
  11. Rohrmann S, Paltoo DN, Platz EA, Hoffman SC, Comstock GW, Helzlsouer KJ. Association of vasectomy and prostate cancer among men in a Maryland cohort. Cancer Causes Control 2005;16:1189-94.
  12. Reddy NM, Gerscovich EO, Jain KA, Le-Petross HT, Brock JM. Vasectomy- related changes on sonographic examination of the scrotum. J Clin Ultrasound 2004;32:394-8.
  13. Shiraishi K, Takihara H, Naito K. Influence of interstitial fibrosis on spermatogenesis after vasectomy and vasovasostomy. Contraception 2002;65:245-9.
  14. Chatterjee S, Laloraya M, Kumar GP. Free radical bombing of spermatozoa in spermatic granuloma: an attempt to prevent autoimmune switch-on. Biochem Biophys Res Commun 1994;201:472-7.
  15. Nourouzi MR, Keyhani AH, Ayati M. Identification of acrosome as the main antigen of the sperm cells provoking autoantibodies in vasectomized iranian men. Acta Medica Iranica 2008;46:457-60.
  16. Gentile GP, Helbig DW, Zacur H, Park T, Lee YJ, Westhoff CL. Hormone levels before and after tubal sterilization. Contraception 2006;73:507-11.
  17. Bedford JM. Adaptations of the male reproductive tract and the fate of spermatozoa following vasectomy in the rabbit, rhesus monkey, hamster and rat. Biol Reprod 1976;14:118-42.
  18. Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford: Clarendon Press; 1985.
  19. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005;3:28.
  20. Alamdari DH, Paletas K, Pegiou T, Sarigianni M, Befani C, Koliakos G. A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients. Clin Biochem 2007;40:248-54.
  21. Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978; 90:37-43.
  22. Chandel NS, McClintock DS, Feliciano CE, Wood TM, Melendez JA, Rodriguez AM, et al. Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1alpha during hypoxia: a mechanism of O2 sensing. J Biol Chem 2000;275:25130-8.
  23. Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 2005;15:316-28.
  24. Ren L, Weng Q, Kishimoto M, Watanabe G, Jaroenporn S, Taya K. Effect of short period vasectomy on FSH, LH, inhibin and testosterone secretions, and sperm motility in adult male rats. Exp Anim 2011;60:47-56.
  25. McGinn JS, Sim I, Bennett NK, McDonald SW. Observations on multiple sperm granulomas in the rat epididymis following vasectomy. Clin Anat 2000;13:185-94.
  26. Aydos K, Kupeli B, Soygur T, Unsal A, Erden E, Tulunay O, et al. Analysis of the relationship between histologic alterations and the generation of reactive oxygen species in vasectomized rat testes. Urology 1998;51:510-5.
  27. Chatterjee S, Rahman MM, Laloraya M, Kumar GP. Sperm disposal system in spermatic granuloma: a link with superoxide radicals. Int J Androl 2001;24:278-83.
  28. Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ, Agarwal A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril 2000;73:459-64.
  29. Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas AJ Jr, et al. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril 2004;81:349-54.
  30. Aitken RJ, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction 2001;122:497-506.
  31. Kubota Y, Sasaki S, Kubota H, Tatsura H, Kohri K. A study on the mechanism of the spermatogenic damage after vasectomy in rats. Nihon Hinyokika Gakkai Zasshi 2001;92:13-22.
  32. Singh SK, Chakravarty S. Histologic changes in the mouse testis after bilateral vasectomy. Asian J Androl 2000;2:115-20.
  33. Rico H, Cuesta C, Cortes J, Seco C, Monteagudo MD, Hernandez ER. Effect of uterine horn ligation on bone mass: an experimental study in rats. Eur J Obstet Gynecol Reprod Biol 2002;102:48-52.
  34. Fagundes ML, Mendes MC, Patta MC, Rodrigues R, Berezowski AT, de Moura MD, et al. Hormonal assessment of women submitted to tubal ligation. Contraception 2005;71:309-14.
  35. Cattanach JF, Milne BJ. Post-tubal sterilization problems correlated with ovarian steroidogenesis. Contraception 1988;38:541-50.
  36. Batista M, Prats N, Calero P, Gonzalez F, Cabrera F, Medrano A, et al. Semen characteristics and plasma levels of testosterone after bilateral vasectomy in bucks. Reprod Domest Anim 2002;37:375-8.
  37. Tanrikut C, Goldstein M, Rosoff JS, Lee RK, Nelson CJ, Mulhall JP. Varicocele as a risk factor for androgen deficiency and effect of repair. BJU Int 2011;108:1480-4.

피인용 문헌

  1. Vasectomy Induces Oxidative Stress and Up-Regulates the Expression of Peroxiredoxins in Mouse Testis in Short and Early Periods after Surgery vol.191, pp.6, 2012, https://doi.org/10.1016/j.juro.2014.01.007