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  • 제12권5호
  • /
  • Pages.189-195
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  • 2012
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  • 1598-2629(pISSN)
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  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
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Vitamin C Induces Apoptosis in Human Colon Cancer Cell Line, HCT-8 Via the Modulation of Calcium Influx in Endoplasmic Reticulum and the Dissociation of Bad from 14-3-$3{\beta}$

  • Kim, Jee Eun (Department of Anatomy, Chung-Ang University College of Medicine) ;
  • Kang, Jae Seung (Department of Anatomy, Seoul National University College of Medicine) ;
  • Lee, Wang Jae (Department of Anatomy, Seoul National University College of Medicine)
  • 투고 : 2012.08.17
  • 심사 : 2012.09.07
  • 발행 : 2012.10.31
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초록

It has been reported that vitamin C plays an effective role in the treatment and prevention of cancer, but its specific mechanisms are still largely unknown. The incidence of colon cancer is now increasing in Korea. Therefore, we have examined here the effect of vitamin C on the induction of the apoptosis on colon cancer and its related mechanisms. We have found that remarkable increase of the apoptosis and the calcium influx in endoplasmic reticulum (ER) in human colon cancer cell line, HCT-8. However, vitamin C-induced apoptosis was effectively inhibited by the pre-treatment of BAPTA-AM (1,2-bis(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid), which is well-known as a calcium specific chelator. During the apoptosis, we found the increase of the translocation of Bad to mitochondria from cytosol, after releasing from 14-3-$3{\beta}$. In this process, the expression of Bax, a well-known pro-apoptotic protein, was also increased. Taken together, vitamin C induces apoptosis of colon cancer cell line, HCT-8 through the increase of 1) the calcium influx in endoplasmic reticulum (ER), 2) the translocation of Bad to mitochondria, and 3) the expression of Bax.

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과제정보

연구 과제 주관 기관 : Ministry of Health, Welfare & Family

참고문헌

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  1. Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells vol.14, pp.None, 2013, https://doi.org/10.1186/1471-2091-14-24
  2. Protective Role of Aspirin, Vitamin C, and Zinc and their Effects on Zinc Status in the DMH-Induced Colon Carcinoma Model vol.14, pp.8, 2012, https://doi.org/10.7314/apjcp.2013.14.8.4627
  3. Intravenous Vitamin C and Cancer : A Systematic Review vol.13, pp.4, 2012, https://doi.org/10.1177/1534735414534463
  4. Effect of Glucose on GLUT1-Dependent Intracellular Ascorbate Accumulation and Viability of Thyroid Cancer Cells vol.67, pp.8, 2015, https://doi.org/10.1080/01635581.2015.1078823
  5. Vitamin C Deficiency Causes Severe Defects in the Development of the Neonatal Cerebellum and in the Motor Behaviors of Gulo−/− Mice vol.23, pp.16, 2015, https://doi.org/10.1089/ars.2014.6043
  6. High concentrations of L-ascorbic acid (Vitamin C) induces apoptosis in a human cervical cancer cell line (HeLa) through the intrinsic and extrinsic pathways vol.86, pp.3, 2012, https://doi.org/10.1893/bios-d-14-00019.1
  7. Vitamin C induces apoptosis in AGS cells via production of ROS of mitochondria vol.12, pp.5, 2012, https://doi.org/10.3892/ol.2016.5212
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  9. Vitamin C and Cancer: The Role of Vitamin C in Disease Progression and Quality of Life in Cancer Patients vol.73, pp.8, 2012, https://doi.org/10.1080/01635581.2020.1795692
  10. The Safe Soluble Compound Dehydroascorbic Acid Inhibits Various Upstream and Downstream Effectors of PI3K and KRAS Signaling Pathways in Undruggable PIK3CA/KRAS-Mutant Colorectal Cancer Stem-Like Cell vol.73, pp.11, 2012, https://doi.org/10.1080/01635581.2020.1856387