Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Ex Vivo Assay of Trace Nicotine Using a Voltammetric Modified Biosensor

  • Ly, Suw Young (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Jang, Myung-Ho (Advenced Scientific Experiment Group in Daejin High School) ;
  • Cha, Jae-Min (Advenced Scientific Experiment Group in Daejin High School) ;
  • Kim, Min-Young (Advenced Scientific Experiment Group in Daejin High School) ;
  • Oh, Hyun-Seok (Advenced Scientific Experiment Group in Daejin High School) ;
  • Jo, Dong-Hyeon (Advenced Scientific Experiment Group in Daejin High School) ;
  • Choi, Seong-Sik (Advenced Scientific Experiment Group in Daejin High School)
  • Received : 2012.01.22
  • Accepted : 2012.03.26
  • Published : 2012.03.30
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Abstract

In vivo nicotine is associated with Alzheimer's, Parkinson's and lung cancer. Diagnostic assays of these diseases depend on very low analytical detection limits. In this study, a sensitive analytical method was examined using a voltammetric graphite pencil electrode (GPE) and a modified carbon nanotube paste electrode (CNE). The optimum analytical conditions for both electrodes were compared using square wave anodic stripping voltammetry (SW) and cyclic voltammetry (CV) obtaining 400 sec accumulation time and oxidation peak. Under optimum parameters, the stripping working range of GPE was $5.0-40.0{\mu}g/L$, CNE: 0.1-0.8 and $5-50{\mu}g/L$. Quantification limits were $5.0{\mu}g/L$ for GPE and $0.1{\mu}g/L$ for CNE, while detection limits were $0.6{\mu}g/L$ for GPE and $0.07{\mu}g/L$ for CNE. A standard deviation of $10.0{\mu}g/L$ was observed for 0.064 GPE and 0.095 CNE (n = 12) using 400 sec accumulation time. The results obtained can be applied to non.treated urine and ex vivo biological diagnostics.

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References

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