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비파엽 추출물의 항산화 효능과 성분분석

Antioxidative Effect and Component Analysis of Eriobotrya japonica Leaf Extracts

  • 김수지 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 박진오 (대봉엘에스(주)) ;
  • 박수남 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소)
  • Kim, Su-Ji (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Jin-O (DAEBONG LS) ;
  • Park, Soo-Nam (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology)
  • 투고 : 2011.11.30
  • 심사 : 2011.12.20
  • 발행 : 2012.03.30

초록

본 연구에서는 비파엽 추출물의 항산화 활성, 타이로시네이즈 저해 활성, 및 추출물/분획의 성분 분석에 대한 연구를 수행하였다. 비파엽 추출물의 자유라디칼(1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성($FSC_{50}$)은 50 % 에탄올 추출물($22.63{\mu}g/mL$) < 에틸아세테이트(ethyl acetate) 분획(6.75) < 당을 제거시킨 아글리콘(aglycone) 분획(5.06) 순으로 증가하였다. 루미놀(luminol)-의존성 화학발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 총항산화능은 에틸아세테이트 분획($OSC_{50}$, $0.75{\mu}g/mL$), 아글리콘 분획(0.79) 및 50 % 에탄올 추출물(1.61)에서 모두 큰 활성을 나타내었다. 비파엽 추출물의 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 보호효과를 조사하였다. 비파엽 추출물은 모든 분획에서 농도 의존적($5{\sim}50{\mu}g/mL$)으로 증가하였으며, 특히 에틸아세테이트 분획은 $10{\mu}g/mL$ 농도에서 ${\tau}50$이 390.8 min, $50{\mu}g/mL$ 농도에서 ${\tau}50$이 1471.5 min으로 높은 세포 보호 활성을 나타내었다. 타이로시네이즈 저해 활성($IC_50$)은 에틸아세테이트 분획($75.25{\mu}g/mL$) < 50 % 에탄올 추출물($74.1{\mu}g/mL$) < 아글리콘 분획($43.35{\mu}g/mL$) 순으로 미백제로 알려진 알부틴보타 타이로시네이즈 저해활성이 크게 나타났다. 비파엽 추출물 중 에틸아세테이트 분획의 TLC 분석 결과 7개의 띠(EJL 1 - EJL 7)가 나타났다. HPLC로 아글리콘 분획을 분석한 결과 kaempferol 및 quercetin이 각각 53.7 % 및 46.3 %였으며, 따라서 추출물 중에는 kaempferol 및 그 배당체 비율이 다소 높은 것으로 나타났다. 이상의 결과들은 비파엽 추출물 또는 분획이 $^1O_2$ 및 다른 ROS를 소거 또는 소광함으로써 그리고 ROS에 대항해서 세포막을 효과적으로 보호함으로써 태양 자외선에 노출된 피부를 보호하는 항산화제로서 작용할 수 있음을 가리키며, 타이로시네이즈 저해 효과로부터 비파엽 추출물 및 분획물은 기능성 화장품 원료로서 응용가능성이 있음을 시사한다.

In the present study, the antioxidative properties, inhibitory activity on tyrosinase, and active components of Eriobotrya japonica (E. japonica) leaf extract were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$) of extract/fraction of E. japonica leaf was in the order 50 % ethanol extract ($22.625{\mu}g/mL$) < ethyl acetate fraction (6.75) < deglycosylated aglycone fraction (5.06). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of fraction/extracton ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescenceassay were investigated. $OSC_{50}$ of the ethyl acetate fraction, deglycosylated aglycone fraction, and ethanol extract were 0.75, 0.79, and $1.61{\mu}g/mL$, respectively. The cellular protective effects of E. japonica leaf extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The protective effects of extract/fraction of E. japonica leaf were increased in a in a concentration dependent manner ($5{\sim}50{\mu}g/mL$). Especially, ${\tau}50$ of ethyl acetate fraction at concentrations of $10{\mu}g/mL$ and $50{\mu}g/mL$ showed the most protective effects at 390.8 min and 1471.5 min. The inhibitory effect ($IC_50$) on tyrosinase of E. japonica leaf extracts was higher than arbutin, known as a skin-whitening agent. The order of inhibitory effects was acetate fraction ($75.25{\mu}g/mL$) < 50 % extract (74.1) < deglycosylated aglycone fraction (43.35). TLC of the ethyl acetate fraction showed 7 bands (EJL 1 - EJL 7). HPLC of the aglycone fraction exhibited 2 peaks, kaempferol and quercetin. The amounts of kaempferol and quercetin were 53.7 and 46.3 %. respectively. Therefore, The amounts of kaempferol and its glucoside were a little bit higher than quercetin and its glucoside in E. japonica leaf extract. Accordingly, these findings suggest that extracts/fractions of E. japonica leaf can function as antioxidants in biological systems, especially skin exposed to UV radiation, and protect cellular membranes against ROS. Thus, the extract/fraction of E. japonica leaf may be used in novel functional cosmetics as antioxidants against skin photoaging.

키워드

참고문헌

  1. L. Packer, Utraviolet radiation (UVA, UVB) and skin antioxidants, In: Free radical damage and its control, eds. C. A. Rice-Evans and R. H. Burdon, Elsevier Science B.V., 239 (1994).
  2. K. Scharffetter-Kochanek, Photoaging of the connective tissue of skin: Its prevention and therapy, In: Antioxidants in disease mechanisms and therapy, eds. H. Sies, Advances Pharmacology, (1997).
  3. S. N. Park, Skin aging and antioxidant, J. Soc. Cosmet. Scientists Korea, 23(1), 75 (1997).
  4. S. N. Park, Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I), J. Korean Ind. Eng. Chem., 14(5), 657 (2003).
  5. Y. J. Ahn, B. R. Won, M. K Kang, J. H. Kim, and S. N. Park, Antioxidant activity and component analysis of fermented Lavendula angustifolia extracts, J. Soc. Cosmet. Scientists Korea, 35(2), 125 (2009).
  6. S. N. Park, S. Y. Kim, G. N. Lim, N. R. Jo, and M. H. Lee, In vitro skin permeation and cellular protective effects of flavonoids isolated from Suaeda asparagoides extracts, J. Ind. Eng. Chem., 18(2), 680 (2012). https://doi.org/10.1016/j.jiec.2011.11.126
  7. Rajindar S. Sohala, William C. Orrb, The redox stress hypothesis of aging, Free Radic. Biol. Med., 52(3), 539 (2012). https://doi.org/10.1016/j.freeradbiomed.2011.10.445
  8. L. C. Magdalena, and Y. A. Tak, Reactive oxygen species, cellular redox systems, and apoptosis, Free Radic. Biol. Med., 48, 749 (2010). https://doi.org/10.1016/j.freeradbiomed.2009.12.022
  9. S. N. Park, Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes, Korea J. Food Sci. Technol., 35(3), 510 (2003).
  10. S. H. Kim and T. Y. Shin, Anti-inflammatory effect of leaves of Eriobotrya japonica correlating with attenuation of p38 MAPK, ERK, and NF-$_{\kappa}B$ activation in mast cells, Toxicology in Vitro, 23, 1215 (2009). https://doi.org/10.1016/j.tiv.2009.07.036
  11. D. S. Cha, T. Y. Shin, J. S. Eun, D. K. Kim, and H. Jeon, Anti-metastatic properties of the leaves of Eriobotrya japonica, Arch. Pharm. Res., 34(3), 425 (2011). https://doi.org/10.1007/s12272-011-0310-1
  12. H. Ito, E. Kobayashi, Y. Takamatsu, S. H. Li, T. Hatano, H. Sakagami, K. Kusama, K. Satoh, D. Sugita, S. Shimura, Y. Itoh, and T. Yoshida, Polyphenols from Eriobotrya japonica and Their Cytotoxicity against Human Oral Tumor Cell Lines, Chem. Pharm. Bull., 48(5), 687 (2000). https://doi.org/10.1248/cpb.48.687
  13. F. Ferreres, D. Gomes, P. Valentao, R. Goncalves, R. Pio, E. A. Chagas, R. M. Seabra, and P. B. Andrade, Improved loquat (Eriobotrya japonica Lindl.) cultivars : Variation of phenolics and antioxidative potential, Food Chemistry, 114, 1019 (2009). https://doi.org/10.1016/j.foodchem.2008.10.065
  14. N. Banno, T. Akihisa, H. Tokuda, K. Yasukawa, Y. Taguchi, H. Akazawa, M. Ukiya, Y. Kimura, T. Suzuki, and H. Nishino, Anti-inflammatory and Antitumor-Promoting Effects of the Triterpene Acids from the Leaves of Eriobotrya japonica, Biol. Pharm. Bull., 28(10), 1995 (2005). https://doi.org/10.1248/bpb.28.1995
  15. Y. G. Hwang, J. J. Lee, A. R Kim, and M. Y. Lee, Chemical components and antioxidative effects of Eriobotrya japonica Lindl. Leaf, J. Life Science, 20(11), 1625 (2010). https://doi.org/10.5352/JLS.2010.20.11.1625
  16. K. Z. Matalka, D. Ali, A. E. Khawad, and F. Qa'dan, The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation, Cytokine, 40, 235 (2007). https://doi.org/10.1016/j.cyto.2007.10.003
  17. T. Uto, N. Suangkaew, O. Morinaga, H. Kariyazono, S. Oiso, and Y. Shoyama, Eriobotryae Folium extract suppresses LPS-induced iNOS and COX-2 expression by inhibition of NF-kappaB and MAPK activation in Murine Macrophages, Am. J. Chin. Med., 38(5), 985 (2010). https://doi.org/10.1142/S0192415X10008408
  18. K. I. Lee and S. M. Kim, Antioxidative and Antimicrobial Activities of Eriobotrya japonica Lindl. Leaf Extracts, J. Korean Soc. Food Sci. Nutr., 38(3), 267 (2009). https://doi.org/10.3746/jkfn.2009.38.3.267
  19. Y. Huang, J. Li, Q. Cao, S. C. Yu, X. W. Lv, Y. Jin, L. Zhang, Y. H. Zou, and J. F. Ge, Anti-oxidative effect of triterpene acids of Eriobotrya japonica (Thunb.), Lindl. leaf in chronic bronchitis rats, Life Sciences, 78, 2749 (2006). https://doi.org/10.1016/j.lfs.2005.10.040

피인용 문헌

  1. A Study on Whitening and Anti-inflammatory Effects of Eriobotrya Japonica Leaf Extracts with Different Extraction Methods vol.41, pp.2, 2015, https://doi.org/10.15230/SCSK.2015.41.2.151
  2. Melanin Synthesis Inhibitory Effect of Eriobotryae Folium Extracts & Eriobotryae Folium and Phreatic Water Mixture vol.38, pp.4, 2017, https://doi.org/10.13048/jkm.17040