Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
권호 표지

Effect of CHT in anti-oxidative and anti-inflammatory related factors

청화탕(淸華湯)의 항산화 및 항염증 효능

  • Kim, Jin-Woo (Dept. of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Gim, Seon-Bin (Traditional and Biomedical Research Center(TBRC), Daejeon University) ;
  • Oh, Jeong-Min (Traditional and Biomedical Research Center(TBRC), Daejeon University) ;
  • Yun, Mi-Young (Dept. of beauty science, Kwangju Women's University) ;
  • Lee, Ki-Moo (Doori Cosmetics) ;
  • Kim, Dong-Hee (Dept. of Pathology, College of Oriental Medicine, Daejeon University)
  • Received : 2011.12.23
  • Accepted : 2012.01.13
  • Published : 2012.02.20
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Abstract

To investigate the clinical aspects of CHT in atopic dermatitis (AD) treatments, the effect of CHT in anti-oxidative and anti-inflammatory cytokines were tested. 100% or higher cell viability was observed in all tested groups from 25 to 200 ${\mu}g/m{\ell}$ using Raw 264.7 cells. CHT showed dose-dependent DPPH scavenging activity, with more than 90% scavenging activities at 800 ${\mu}g/m{\ell}$ concentrations. CHT showed dose-dependent suppression activity of ROS production, especially at 200 ${\mu}g/m{\ell}$ of 37.5%. CHT decreased NO production activity, with significant decrease of 33.2% at 200 ${\mu}g/m{\ell}$. IL-6, MCP-1, TNF-${\alpha}$ production rate were decreased by approximately 25% when Raw 264.7 cells were treated with LPS and with CHT of 200 ${\mu}g/m{\ell}$. Also, IL-$1{\beta}$ production rate was decreased by 25% at 100 ${\mu}g/m{\ell}$. The results above indicate that CHT significantly reduces the effect of oxidative and inflammatory cytokines. The use of CHT in dermatitis can be widely suggested.

Keywords

References

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