Mycobiology

  • 제40권1호
  • /
  • Pages.47-52
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  • 2012
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Antitumor and Immunostimulating Activities of $Elfvingia$ $applanata$ Hot Water Extract on Sarcoma 180 Tumor-bearing ICR Mice

  • Shim, Sung-Mi (Division of Life Sciences, University of Incheon) ;
  • Lee, Jae-Seong (Division of Life Sciences, University of Incheon) ;
  • Lee, Tae-Soo (Division of Life Sciences, University of Incheon) ;
  • Lee, U-Youn (Division of Life Sciences, University of Incheon)
  • 투고 : 2012.03.12
  • 심사 : 2012.03.12
  • 발행 : 2012.03.31
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초록

$Elfvingia$ $applanata$, a medicinal mushroom belonging to Basidiomycota, has been used in the effort to cure cancers of the esophagus and stomach, and is also known to have inhibitory effects on hepatitis B virus infection. The hot water soluble fraction (as Fr. HW) was extracted from fruiting bodies of the mushroom. $In$ $vitro$ cytotoxicity tests showed that hot water extract was not cytotoxic against cancer cell lines such as Sarcoma 180, HT-29, HepG2, and TR at concentrations of 10-2,000 ${\mu}g/mL$. Intraperitoneal injection with Fr. HW resulted in a life prolongation effect of 45.2% in mice previously inoculated with Sarcoma 180. Treatment of Fr. HW resulted in a 2.53-fold increase in the numbers of murine spleen cells at a concentration of 50 ${\mu}g/mL$, compared with control. Incubation of murine spleen cells with Fr. HW at a concentration of 500 ${\mu}g/mL$ resulted in improved immune-potwntiating activity of B lymphocytes through an 8.3-folds increase in alkaline phosphatase activity, compared with control. Fr. HW generated 12.5 ${\mu}M$ of nitric oxide (NO) when cultured with RAW 264.7, a mouse macrophage cell line, at the concentration of 50 ${\mu}g/mL$, while lipopolysaccharide, a positive control, produced 15.2 ${\mu}M$ of NO. Therefore, the results suggested that antitumor activities of Fr. HW from $E.$ $applanata$ might, in part, be due to host mediated immunostimulating activity.

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