DOI QR코드

DOI QR Code

Study of Sugarcane Pieces as Yeast Supports for Ethanol Production from Sugarcane Juice and Molasses Using Newly Isolated Yeast from Toddy Sap

  • 투고 : 2011.11.01
  • 심사 : 2012.01.18
  • 발행 : 2012.03.31

초록

A repeated batch fermentation system was used to produce ethanol using $Saccharomyces$ $cerevisiae$ strain (NCIM 3640) immobilized on sugarcane ($Saccharum$ $officinarum$ L.) pieces. For comparison free cells were also used to produce ethanol by repeated batch fermentation. Scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of the yeast to the surface of the sugarcane pieces. Repeated batch fermentations using sugarcane supported biocatalyst were successfully carried out for at least ten times without any significant loss in ethanol production from sugarcane juice and molasses. The number of cells attached to the support increased during the fermentation process, and fewer yeast cells leaked into fermentation broth. Ethanol concentrations (about 72.65-76.28 g/L in an average value) and ethanol productivities (about 2.27-2.36 g/L/hr in an average value) were high and stable, and residual sugar concentrations were low in all fermentations (0.9-3.25 g/L) with conversions ranging from 98.03-99.43%, showing efficiency 91.57-95.43 and operational stability of biocatalyst for ethanol fermentation. The results of the work pertaining to the use of sugarcane as immobilized yeast support could be promising for industrial fermentations.

키워드

참고문헌

  1. Suresh K, Kiransree N, Rao LV. Production of ethanol by raw starch hydrolysis and fermentatation of damaged grains of wheat and sorghum. Bioprocess Eng 1999;21:165-8. https://doi.org/10.1007/s004490050657
  2. Suresh K, Sree NK, Rao LV. Utilization of damaged sorghum and rice grains for ethanol production by simultaneous saccharification and fermentation. Bioresour Technol 1999; 68:301-4. https://doi.org/10.1016/S0960-8524(98)00135-7
  3. Chaudhary AB, Chincholkar SB. Cell immobilization a critical approach to ethanol production by Saccharomyces cerevisiae and Schizosaccharomyces pombe. Indian J Microbiol 1996;36:75-83.
  4. Sree NK, Sridhar M, Suresh K, Banat IM, Rao LV. High alcohol production by repeated batch fermentation using an immobilized osmotolerant Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 2000;24:222-6. https://doi.org/10.1038/sj.jim.2900807
  5. Margaritis A, Merchant FJ. Advances in ethanol-production using immobilized cell systems. CRC Crit Rev Biotechnol 1984;1:339-93.
  6. Corton E, Piuri M, Battaglini F, Ruzal SM. Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cells technique. Biotechnol Prog 2000;16:59-63. https://doi.org/10.1021/bp9901217
  7. Kourkoutas Y, Komaitis M, Koutinas AA, Kanellaki M. Wine production using yeast immobilized on apple pieces at low and room temperatures. J Agric Food Chem 2001; 49:1417-25. https://doi.org/10.1021/jf000942n
  8. Kourkoutas Y, Kanellaki M, Koutinas AA. Apple pieces as immobilization support of various microorganisms. LWTFood Sci Technol 2006:39:980-6.
  9. Kourkoutas Y, McErlean C, Kanellaki M, Hack CJ, Marchant R, Banat IM, Koutinas AA. High-temperature wine making using the thermotolerant yeast strain Kluyveromyces marxianus IMB3. Appl Biochem Biotechnol 2004;112:25-35. https://doi.org/10.1385/ABAB:112:1:25
  10. Kourkoutas Y, Xolias V, Kallis M, Bezirtzoglou E, Kanellaki M. Lactobacillus casei cell immobilization on fruit pieces for probiotic additive, fermented milk and lactic acid production. Process Biochem 2005;40:411-6. https://doi.org/10.1016/j.procbio.2004.01.029
  11. Plessas S, Bekatorou A, Koutinas AA, Soupioni M, Banat IM, Marchant R. Use of Saccharomyces cerevisiae cells immobilized on orange peel as biocatalyst for alcoholic fermentation. Bioresour Technol 2007;98:860-5. https://doi.org/10.1016/j.biortech.2006.03.014
  12. Chronopoulos G, Bekatorou A, Bezirtzoglou E, Kaliafas A, Koutinas AA, Marchant R, Banat IM. Lactic acid fermentation by Lactobacillus casei in free cell form and immobilised on gluten pellets. Biotechnol Lett 2002;24:1233-6. https://doi.org/10.1023/A:1016274121642
  13. de Vasconcelos JN, Lopes CE, de Franca FP. Continuous ethanol production using yeast immobilized on sugar-cane stalks. Braz J Chem Eng 2004;21:357-65. https://doi.org/10.1590/S0104-66322004000300002
  14. Liang L, Zhang YP, Zhang L, Zhu MJ, Liang SZ, Huang YN. Study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses. J Ind Microbiol Biotechnol 2008;35:1605-13. https://doi.org/10.1007/s10295-008-0404-z
  15. Reddy LV, Reddy LP, Wee YJ, Reddy OV. Production and characterization of wine with sugarcane piece immobilized yeast biocatalyst. Food Bioprocess Technol 2011;4:142-8. https://doi.org/10.1007/s11947-009-0321-9
  16. Reddy LV, Reddy YH, Reddy LP, Reddy OV. Wine production by novel yeast biocatalyst prepared by immobilization on watermelon (Citrullus vulgaris) rind pieces and characterization of volatile compounds. Process Biochem 2008;43:748-52. https://doi.org/10.1016/j.procbio.2008.02.020
  17. Reddy LV, Reddy YH, Reddy OV. Wine production by guava piece immobilized yeast from Indian cultivar grapes and its volatile composition. Biotechnology 2006;5:449-54. https://doi.org/10.3923/biotech.2006.449.454
  18. Bardi EP, Koutinas AA. Immobilization of yeast on delignified cellulosic material for room termperature and lowtemperature wine making. J Agric Food Chem 1994;42:221-6. https://doi.org/10.1021/jf00037a040
  19. Kopsahelis N, Agouridis N, Bekatorou A, Kanellaki M. Comparative study of spent grains and delignified spent grains as yeast supports for alcohol production from molasses. Bioresour Technol 2006;98:1440-7.
  20. Sahin HT, Arslan MB. A study on physical and chemical properties of cellulose paper immersed in various solvent mixtures. Int J Mol Sci 2008:9:78-88. https://doi.org/10.3390/ijms9010078
  21. Tanaka A, Kawamoto T. Cell and enzyme immobilization. In: Demain AL, Davies JE, Atlas RM, Cohen G, Hershberger CL, Hu WS, Sherman DH, Wilson RC, Wu JH, editors. Manual of industrial microbiology and biotechnology. 2nd ed. Washington, DC: ASM Press; 1999. p. 94-102.
  22. Lee SS, Robinson FM, Wang HY. Rapid determination of yeast viability. Biotechnol Bioeng Symp 1981;11:641-9.
  23. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-8. https://doi.org/10.1021/ac60147a030
  24. Peinado RA, Moreno JJ, Villalba JM, Gonzalez-Reyes JA, Ortega JM, Mauricio JC. Yeast biocapsules: a new immobilization method and their applications. Enzyme Microb Technol 2006;40:79-84. https://doi.org/10.1016/j.enzmictec.2005.10.040
  25. Sobocan G, Glavic P. Optimization of ethanol fermentation process design. Appl Therm Eng 2000;20:529-43. https://doi.org/10.1016/S1359-4311(99)00042-3
  26. Olguin EJ, Doelle HW, Mercado G. Resource recovery through recycling of sugar processing by-products and residuals. Resour Conserv Recycl 1995;15:85-94. https://doi.org/10.1016/0921-3449(95)00028-H
  27. Nagashima M, Azuma M, Noguchi S, Inuzuka K, Samejima H. Continuous ethanol fermentation using immobilized yeast cells. Biotechnol Bioeng 1984;26:992-7. https://doi.org/10.1002/bit.260260826
  28. Bekatorou A, Sarellas A, Ternan NG, Mallouchos A, Komaitis M, Koutinas AA, Kanellaki M. Low-temperature brewing using yeast immobilized on dried figs. J Agric Food Chem 2002; 50:7249-57. https://doi.org/10.1021/jf020291q