Genomics & Informatics

  • 제10권1호
  • /
  • Pages.40-43
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  • 2012
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  • 1598-866X(pISSN)
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  • 2234-0742(eISSN)
한국유전체학회 (Korea Genome Organization)
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Decreases in $Casz1$ mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice

  • Ji, Su-Min (Department of Biomedical Engineering, Kyung Hee University School of Medicine) ;
  • Shin, Young-Bin (Department of Biomedical Engineering, Kyung Hee University School of Medicine) ;
  • Park, So-Yon (Department of Biomedical Engineering, Kyung Hee University School of Medicine) ;
  • Lee, Hyeon-Ju (Department of Biomedical Engineering, Kyung Hee University School of Medicine) ;
  • Oh, Berm-Seok (Department of Biomedical Engineering, Kyung Hee University School of Medicine)
  • 투고 : 2011.12.21
  • 심사 : 2012.02.08
  • 발행 : 2012.03.31
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초록

Recent genomewide association studies of large samples have identified genes that are associated with blood pressure. The Global Blood Pressure Genetics (Global BPgen) and Cohorts for Heart and Aging Research in Genome Epidemiology (CHARGE) consortiums identified 14 loci that govern blood pressure on a genomewide significance level, one of which is $CASZ1$ confirmed in both Europeans and Asians. $CASZ1$ is a zinc finger transcription factor that controls apoptosis and cell fate and suppresses neuroblastoma tumor growth by reprogramming gene expression, like a tumor suppressor. To validate the function of $CASZ1$ in blood pressure, we decreased $Casz1$ mRNA levels in mice by siRNA. $Casz1$ siRNA reduced mRNA levels by 59% in a mouse cell line. A polyethylenimine-mixed siRNA complex was injected into mouse tail veins, reducing $Casz1$ mRNA expression to 45% in the kidney. However, blood pressure in the treated mice was unaffected, despite a 55% reduction in $Casz1$ mRNA levels in the kidney on multiple siRNA injections daily. Even though $Casz1$ siRNA-treated mice did not experience any significant change in blood pressure, our study demonstrates the value of $in$ $vivo$ siRNA injection in analyzing the function of candidate genes identified by genomewide association studies.

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참고문헌

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  2. Gene Silencing and Haploinsufficiency of Csk Increase Blood Pressure vol.11, pp.1, 2016, https://doi.org/10.1371/journal.pone.0146841
  3. GAREM1 regulates the PR interval on electrocardiograms vol.63, pp.3, 2018, https://doi.org/10.1038/s10038-017-0367-x