Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anti-Proliferative Effect of Ethanol on Normal and Cancer Cells

정상세포와 암세포의 증식 억제에 대한 에탄올의 영향

  • Oh, Myung-Ju (Division of Clinical Trials Management, Bureau of Risk Prevention Policy, Korea Food and Drug Administration) ;
  • Kim, Ji-Hyun (Department of Nanomedical Engineering, Pusan National University) ;
  • Park, Su-Hyun (Department of Nanomedical Engineering, Pusan National University) ;
  • Jeong, Young-Hwa (Department of Nanomedical Engineering, Pusan National University) ;
  • Wang, Kun (Department of Animal Sciences, Pusan National University) ;
  • Cho, Byung-Wook (Department of Animal Sciences, Pusan National University) ;
  • Jhun, Byung-H. (Department of Nanomedical Engineering, Pusan National University)
  • 오명주 (식품의약청 임상시험관리부) ;
  • 김지현 (부산대학교 나노과학기술대학 나노메디컬공학과) ;
  • 박수현 (부산대학교 나노과학기술대학 나노메디컬공학과) ;
  • 정영화 (부산대학교 나노과학기술대학 나노메디컬공학과) ;
  • 왕쿤 (부산대학교 생명자원과학대학 동물자원학과) ;
  • 조병욱 (부산대학교 생명자원과학대학 동물자원학과) ;
  • 전병학 (부산대학교 나노과학기술대학 나노메디컬공학과)
  • Received : 2012.02.17
  • Accepted : 2012.03.22
  • Published : 2012.04.30
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Abstract

Ethanol is known as being carcinogenic to humans. In addition, the anti-proliferative effects of ethanol have been described for a variety of tissues and cells. In this study, we investigated the anti-proliferative effects of ethanol on various cancer cells, particularly on oncogenic $ras$-transformed or-injected cells. Ethanol treatment inhibited the cell proliferation of normal control cells, but did not suppress the proliferation of various cancer cells and oncogenic $ras$-transformed cells. Furthermore, ethanol treatment did not interfere with DNA synthesis, which was induced by microinjecting the oncogenic $H-Ras^{V12}$ protein. The anti-proliferative effect of ethanol was rescued by antioxidants, such as $N$-acetylcysteine and 4-methlpyrazole. These results suggest that ethanol cytotoxicity is exerted through free radical formation, and that the anti-proliferative action site of ethanol cytotoxicity either lies upstream, or is independent of Ras.

에탄올은 사람에 대한 발암물질로 잘 알려져 있다. 또한 여러 조직이나 세포에서의 에탄올에 의한 세포증식억제효과도 잘 알려져 있다. 본 연구에서는 여러 암세포에서 에탄올에 의한 세포증식억제 효과를 조사하였는데 특히 발암원성 $ras$로 형질전환되거나 미세주입된 세포에서의 영향을 조사하였다. 에탄올은 여러 정상세포들의 증식을 억제하였다. 반면에 여러 암세포나 발암원성 Ras에 의한 세포증식은 억제하지 못 하였다. 또한 발암원성 단백질의 세포내 미세주사에 의한 DNA합성 유도도 에탄올에 의해 억제 되지 않았다. 이러한 에탄올의 세포증식억제 효과는 $N$-acetylcysteine이나 4-methylpyrazole과 같은 항산화제에 의해 제거되었다. 이러한 실험 결과는 에탄올에 의한 세포증식억제 효과는 Ras단백질의 upstream에 있거나 또는 Ras와 독립적으로 작용하며, 활성산소 형성과 밀접한 관계가 있다는 것을 알려준다.

Keywords

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