생명과학회지 (Journal of Life Science)

  • 제22권4호
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  • Pages.486-491
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Myostatin prodomains이 rotifer 생활사에 미치는 영향

Effects of Myostatin Prodomains on the Reproduction of Rotifer Brachionus rotundiformis

  • 조미진 (강릉원주대학교 생명과학대학 해양분자생명공학과) ;
  • 진형주 (강릉원주대학교 생명과학대학 해양분자생명공학과)
  • Jo, Mi-Jin (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Jin, Hyung-Joo (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University)
  • 투고 : 2012.02.16
  • 심사 : 2012.04.22
  • 발행 : 2012.04.30
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초록

Myostatin (MSTN)은 TGF (transforming growth factor)-beta family에 속하며, 골격근 성장의 억제 조절인자로서 여러 포유류에서 MSTN 유전자 돌연변이는 골격근 증가를 유도한다. MSTN prodomain은 MSTN의 생물학적 활성을 저해하는데, MSTN prodomain이 과 발현된 쥐에서 과도한 근육축적이 확인되었다. 로티퍼(rotifer; Brachionus rotundiformis)는 치어기 어류의 양식산업에 있어 주요한 일차적 먹이생물이다. 그러나 로티퍼에서 MSTN 및 MSTN prodomain의 기능과 발현 유무는 알려져 있지 않다. 따라서 본 연구는 재조합 MSTN prodomains이로티퍼에 미치는 영향에 관하여 조사하고자 하였다. 로티퍼 개체배양 실험을 통하여 재조합 MSTN prodomains(pMALc2x-poMSTNpro, pAMLc2x-sMSTNpro)에 의한 로티퍼의 생식 전 단계, 순 생식단계, 생식 후 단계, 산란, 수명, 포란, 수컷 발생률을 확인하였으며, 또한 pMALc2x-poMSTNpro와 pAMLc2x-sMSTNpro이 밀집배양에서 로티퍼의 개체성장에 영향을 미치는지에 대하여 확인하였다. 그 결과 농도가 1, 2, 4 ${\mu}g/ml$에서 pMALc2x-poMSTNpro를 처리한 실험군과 0.25 ${\mu}g/ml$에서 4 ${\mu}g/ml$ 농도까지 pMALc2x-sMSTNpro를 처리한 실험군에서 로티퍼의 생식 전 단계가 아무처리하지 않은 대조군에 비하여 짧아졌다. 밀집배양 실험에 있어 pMALc2x-poMSTNpro와 pMALc2x-sMSTNpro 모두 로티퍼의 개체 수를 증가를 유도하여, 재조합 MSTN prodomains에 의해서 로티퍼의 reprodution에 영향을 주는 것으로 나타났다. 하지만, 재조합 MSTN prodomains이 어떠한 수용체를 이용하여 신호를 전달하는지에 대한 연구는 앞으로 더 진행되어야 하며, 본 연구의 결과는 재조합 MSTN prodomains이 미세조류에서의 기능 및 메커니즘연구에 중요한 기초자료가 될 것으로 사료된다.

Myostatin (MSTN), a member of the transforming growth factor (TGF)-beta family, is a potent negative regulator of skeletal muscle growth and maintenance. The MSTN prodomain inhibits MSTN biological activity. The rotifer Brachionus rotundiformis is an excellent primary live feed for fish larvae in aquaculture; however, it is not known whether the rotifer expresses MSTN and the MSTN prodomain along with its activity. The objective of this study was to examine the effects of recombinant MSTN prodomains. Individual cultures of the rotifer B. rotundiformis were carried out to determine the effect of recombinant MSTN prodomains (pMALc2x-poMSTNpro and pMALc2x-sMSTNpro) on the pre-reproductive phase, reproductive phase, post-reproductive phase, offspring, lifespan, fecundity, and male ratio. In addition, a population culture of the rotifer was performed to confirm the effects of pMALc2x-poMSTNpro and pMALc2x-sMSTNpro on population growth. The results showed that the rotifer treated with pMALc2x-pMSTNpro had a reduced pre-reproductive phase at higher concentrations (1, 2, and 4 ${\mu}g/ml$) compared to the non-treated control group. Moreover, the pMALc2xsMSTNpro treated rotifer effectively decreased the pre-reproductive phase at a lower concentration (0.25 ${\mu}g/ml$) compared to the pMALc2x-pMSTNpro treated and control group. Interestingly, pMALc2x-poMSTNpro and pMALc2x-sMSTNpro significantly increased the population of $B.$ $rotundiformis$.

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참고문헌

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