Journal of Mushroom (한국버섯학회지)
- Volume 10 Issue 1
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- Pages.3-8
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- 2012
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- 1738-0294(pISSN)
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- 2288-8853(eISSN)
DOI QR Code
Changes in the CO2 and amount of mycelium growth of the liquid spawn on Flammulina velutipes
팽나무버섯 액체 종균 배양시 이산화탄소 농도와 균사 생장량 변화
- Shim, Kyu-Kwang (ParoTech) ;
- Yoo, Young-Jin (Jeollabuk-do Agricultural Research and Crop-Management) ;
- Koo, Chang-Duck (Chungbuk National Univ.) ;
- Kim, Young-Seok (Jeonbuk National Univ., Department of Agriculture, Agrochimie.) ;
- Kim, Myung-Koon (Jeonbuk National Univ. Iksan Campus, Bio Food Technology)
- Received : 2012.03.04
- Accepted : 2012.03.21
- Published : 2012.03.31
Abstract
In this study, to produce Flammulina velutipes mushroom liquid spawn efficiently and effectively the effects of explosive aeration (supplying air with tiny bubbles) of the liquid culture medium on carbon dioxide concentration and residual sugar content in the medium on carbon dioxide concentration and residual sugar contentin the medium were measured. Carbon dioxide concentrations were measured at the outlet of the incubator. On the third day the explosive aeration greatly increased mycelial growth of the liquid spawn, and carbon dioxide concentration also greatly increased but decreased after 5 days. Free sugar contents in the liquid culture consistantly decreased up to 7 days and thereafter was not detected. The weight of the mycelia were maintained similar levels after 3 days. Total nitrogen content in the liquid medium constantly decreased during the 11days of explosive aeration. The content of free sugars in 7 days of culture was the lowest level, thus the inoculum incubated for 6~7 days was thought to be the most effective. Carbon dioxide concentration measurement at the outlet of the container during the liquid spawn incubation required low cost but was efficient to estimate the degree of mycelial growth to be used as a simple indicator.
본 연구에서는 효율적으로 팽나무버섯 액체종균을 생산할 때에 폭기(공기방울을 매우 작게하여) 배양의 효과에 대하여 액체 배지 내 이산화탄소 농도와 잔류 유리당 함량 변화로써 측정하였다. 이산화탄소 농도는 폭기 진행 중 배양용기의 배출구에서 측정하였다. 액체종균은 폭기 배양 3일째에 균사생장이 급격히 증가하였으며, 이산화탄소 농도(
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