Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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In vitro Shoot Propagation Derived from Stem and Shoot Tip in Hovenia dulcis var. koreana Nakai by Plnat Growth Regulators and Light Resources

식물생장조절제 및 광원처리에 따른 헛개나무 줄기와 경정유래 신초의 기내증식

  • Park, Mi-Young (Department of Horticultual Biotechnology, College of Life Science, Kyung Hee University) ;
  • Wang, Fengbo (Department of Horticultual Biotechnology, College of Life Science, Kyung Hee University) ;
  • Eom, Seok-Hyun (Department of Horticultual Biotechnology, College of Life Science, Kyung Hee University) ;
  • Lee, Seung-Woo (Department of Horticultual Biotechnology, College of Life Science, Kyung Hee University)
  • 박미영 (경희대학교 생명과학대학 원예생명공학과) ;
  • 왕펑보 (경희대학교 생명과학대학 원예생명공학과) ;
  • 엄석현 (경희대학교 생명과학대학 원예생명공학과) ;
  • 이승우 (경희대학교 생명과학대학 원예생명공학과)
  • Received : 2011.12.15
  • Accepted : 2012.02.08
  • Published : 2012.02.29
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Abstract

This study was conducted to examine effects of plant growth regulators and light resources on the formation of multiple shoot and plant regeneration of Hovenia dulcis var. koreana Nakai. Stem and shoot tip were cultured on MS medium or WPM supplemented with various plant growth regulators. At the single treatment, the highest shoot formation was obtained when stem explants were cultured on WPM supplemented with kinetin $1.0mg{\cdot}L^{-1}$. MS medium containing NAA 0.1 and TDZ $0.1mg{\cdot}L^{-1}$ gave the best results for shoot induction rate and shoot growth in combination treatments. Of the BAP and kinetin tested, BAP $0.5mg{\cdot}L^{-1}$ on WPM was found to be more effective for shoot growth from shoot tip. Under white fluorescent light treatment, shoot growth was much higher than blue, red LED treatments. Root induction from in vitro growth of plantlet was the best on WPM supplemented with $1.0mg{\cdot}L^{-1}$ IBA. The results suggest that selection of plant growth regulators and light resources could be important factor to achieve an efficient in vitro growth.

Keywords

References

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