Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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The Comparison of Survival Rates of Vitrified Mouse Blastocysts in Various Vitrification Solutions and Apparatuses

다양한 유리화 용액과 동결기구에서 유리 동결 생쥐 포배기 배아의 생존율 비교

  • Cho, Dong-Hyu (Department of Obstetrics and Gynecology, Chonbuk National University Medical School) ;
  • Lee, Ky-Sook (Department of Obstetrics and Gynecology, Chonbuk National University Medical School) ;
  • Rheu, Chul-Hee (Department of Obstetrics and Gynecology, Chonbuk National University Medical School) ;
  • Kwon, Jung-Kee (Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Jeong-Heon (Department of Obstetrics and Gynecology, Chonbuk National University Medical School)
  • 조동휴 (전북대학교 의학전문대학원 산부인과학교실) ;
  • 이기숙 (전북대학교 의학전문대학원 산부인과학교실) ;
  • 류철희 (전북대학교 의학전문대학원 산부인과학교실) ;
  • 권중기 (전북대학교 수의과대학 실험동물의학교실) ;
  • 이정헌 (전북대학교 의학전문대학원 산부인과학교실)
  • Accepted : 2012.02.20
  • Published : 2012.02.29
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Abstract

The purpose of this study was to evaluate survival rates of vitrified mouse blastocysts in various vitrification solutions (cryoprotectants) and apparatuses. The mouse blastocysts were harvested from culture of mouse 2 cell embryo and were divided into three group (i) untreated (control); (ii) exposed to cryoprotectant agents; or (iii) cryopreserved by various vitrification apparatuses. Vitrification solutions are 40% ethylene glycol (EG) + 5.8 mg/mL ficoll + 0.5M sucrose (EFS solution), 3M glycerol + 3M EG (ES solution), 20% EG + 20% dimethyl sulfoxide (ED solution), 3M EG + 1.0 m sucrose (ES solution). Vitrification apparatuses consisted of 5 groups ; closed plastic straw (CPS), electron microscope (EM) grid, cryoloop, open pulled straw (OPS), and glass micropippete in plastic straw (GPS). The survival rates of control were 88.0%. The survival rates of exposed blastocysts in EFS, GE, ED, and ES solutions were 70.8%, 43.5% (P<0.01), 83.3% and 65.2%, respectively. The survival rates of vitrified blastocysts in CPS, EM grid, cryoloop, OPS and GPS were 56.5% (P< 0.01), 72.7%, 83.3%, 60.9% (P<0.05) and 54.2% (P<0.01), respectively. Among the vitrification solutions, the highest survival rate was seen in blastocysts vitrified in EG + DMSO (83.3%). The survival rate was not significantly different from that of the control (88%). Blastocysts cryopreserved with glycerol in all groups had an overall low survival rate of 43.5%. Survival rate of mouse blastocysts between vitrification apparatuses showed higher in cryoloop.

Keywords

References

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