Journal of Microbiology and Biotechnology

  • 제22권11호
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  • Pages.1580-1587
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Protective Immunity of Pichia pastoris-Expressed Recombinant Envelope Protein of Japanese Encephalitis Virus

  • Kwon, Woo-Taeg (Department of Environmental Health and Safety, Eulji University) ;
  • Lee, Woo-Sik (Department of Environment and Energy Engineering, Gachon University) ;
  • Park, Pyo-Jam (Department of Biotechnology, Research Institute for Biomedical and Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Park, Tae-Kyu (Department of Biotechnology, Research Institute for Biomedical and Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Kang, Hyun (Department of Biotechnology, Research Institute for Biomedical and Health Science, College of Biomedical and Health Science, Konkuk University)
  • 투고 : 2012.05.21
  • 심사 : 2012.07.05
  • 발행 : 2012.11.28
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초록

Japanese encephalitis virus (JEV) envelope (E) protein holds great promise for use in the development of a recombinant vaccine. Purified recombinant E (rE) protein may be useful for numerous clinical applications; however, there are limitations in using the Escherichia coli expression system for producing high-quality rE protein. Therefore, in this study, the yeast expression system was used to generate the rE protein. For protein production using the yeast system, the full-length JEV E gene was cloned into Pichia pastoris. SDS-PAGE and immunoblotting analysis demonstrated that the rE protein had a molecular mass of 58 kDa and was glycosylated. The predicted size of the mature unmodified E protein is 53 kDa, suggesting that post-translational modifications resulted in the higher molecular mass. The rE protein was purified to greater than 95% purity using combined ammonium sulfate precipitation and a SP-Sepharose Fast Flow column. This purified rE protein was evaluated for immunogenicity and protective efficacy in mice. The survival rates of mice immunized with the rE protein were significantly increased over that of Hyphantria cunea nuclear polyhedrosis virus E protein (HcE). Our results indicate that the rE protein expressed in the P. pastoris expression system holds great promise for use in the development of a subunit vaccine against JEV.

키워드

참고문헌

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피인용 문헌

  1. Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in Pichia pastoris vol.5, pp.5, 2012, https://doi.org/10.1016/j.phrp.2014.08.005
  2. Recombinant protein subunit vaccine synthesis in microbes: a role for yeast? vol.67, pp.3, 2012, https://doi.org/10.1111/jphp.12353
  3. Recent advances in the production of recombinant subunit vaccines inPichia pastoris vol.7, pp.3, 2012, https://doi.org/10.1080/21655979.2016.1191707
  4. Efficient production of recombinant glycoprotein D of herpes simplex virus type 2 in Pichia pastoris and its protective efficacy against viral challenge in mice vol.162, pp.3, 2017, https://doi.org/10.1007/s00705-016-3154-7
  5. Cloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcript-2 with tuftsin in Pichia pastoris vol.48, pp.9, 2012, https://doi.org/10.1080/10826068.2018.1514511
  6. Effective Neutralizing Antibody Produced in Mice Directly Immunized with Integrated Pichia pastoris Expressing HPV16L1 Protein vol.32, pp.7, 2012, https://doi.org/10.1089/vim.2019.0055