Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Microbial Community in the Leachate Associated with the Decomposition of Entombed Pigs

  • Yang, Seung-Hak (Animal Environment Division, National Institute of Animal Science, R.D.A) ;
  • Hong, Sun Hwa (Department of Environmental and Energy Engineering, Suwon University) ;
  • Cho, Sung Back (Animal Environment Division, National Institute of Animal Science, R.D.A) ;
  • Lim, Joung Soo (Department of Environmental and Energy Engineering, Suwon University) ;
  • Bae, Sung Eun (Women's Health, School of Medicine, Hodgkin Building, King's College London) ;
  • Ahn, Heekwon (Department of Animal Biosystems Science, Chungnam National University) ;
  • Lee, Eun Young (Department of Environmental and Energy Engineering, Suwon University)
  • Received : 2012.05.04
  • Accepted : 2012.06.20
  • Published : 2012.10.28
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Abstract

Foot and mouth disease (FMD) is one of the acute infectious diseases in hoofed and even-toed mammals, including pigs, and it occurs via acute infection by Aphthovirus. When FMD is suspected, animals around the location of origin are typically slaughtered and buried. Other methods such as rendering, composting, and incineration have not been verified in practice in Korea. After the FMD incident, the regular monitoring of the microbial community is required, as microorganisms greatly modify the characteristics of the ecosystem in which they live. This is the result of their metabolic activities causing chemical changes to take place in the surrounding environment. In this study, we investigated changes in the microbial community during a 24 week period with DNA extracts from leachate, formed by the decomposition of buried pigs at a laboratory test site, using denaturing gradient gel electrophoresis (DGGE) with a genomic DNA. Our results revealed that Bacteroides coprosuis, which is common in pig excreta, and Sporanaerobacter acetigenes, which is a sulfur-reduced microbe, were continuously observed. During the early stages (0~2 weeks) of tissue decomposition, Clostridium cochlearium, Fusobacterium ulcerans, and Fusobacterium sp., which are involved in skin decomposition, were also observed. In addition, various microbes such as Turicibacter sanguinis, Clostridium haemolyticum, Bacteroides propionicifaciens, and Comamonas sp. were seen during the later stages (16~24 weeks). In particular, the number of existing microbial species gradually increased during the early stages, including the exponential phase, decreased during the middle stages, and then increased again during the later stages. Therefore, these results indicate that the decomposition of pigs continues for a long period of time and leachate is created continuously during this process. It is known that leachate can easily flow into the neighboring environment, so a long-term management plan is needed in burial locations for FMD-infected animals.

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References

  1. An, Y. J., Y. H. Joo, I. Y. Hong, H. W. Ryu, and K. S. Cho. 2004. Microbial characterization of toluene-degrading denitrifying consortia obtained from terrestrial and marine ecosystems. Appl. Microbiol. Biotechnol. 65: 611-619.
  2. Atsuko, U., A. Kunihiro, K. Nobuo, W. Kazuya, and U. Katsuji. 2008. Bacteroides propionicifaciens sp. nov., isolated from ricestraw residue in a methanogenic reactor treating waste from cattle farms. Int. J. Syst. Evol. Microbiol. 58: 346-352. https://doi.org/10.1099/ijs.0.65486-0
  3. Bennett, K. W. and A. Eley. 1993. Fusobacteria: New taxonomy and related diseases. J. Med. Microbiol. 39: 246-254. https://doi.org/10.1099/00222615-39-4-246
  4. Brooksby, J. B. 1982. Portraits of viruses: Foot-and-mouth disease virus. Intervirology 18: 1-23. https://doi.org/10.1159/000149299
  5. Carter, D. O. and M. Tibbett. 2006. Microbial decomposition of skeletal muscle tissue (Ovis aries) in a sandy loam soil at different temperatures. Soil Biol. Biochem. 38: 1139-1145. https://doi.org/10.1016/j.soilbio.2005.09.014
  6. Domingo, E., M. G. Mateu, M. A. Martínez, J. Dopazo, A. Moya, and F. Sobrino. 1990. Genetic variability and antigenic diversity of foot-and-mouth disease virus, pp. 233-266. In Applied Virology Research, Virus Variability Epidemiology and control, Vol. 2.
  7. Etchebehere, C., M. I. Errazquin, P. Dabert, R. Moletta, and L. Muxi. 2001. Comamonas nitrativorans sp. nov., a novel denitrifier isolated from a denitrifying reactor treating landfill leachate. Int. J. Syst. Evol. Microbiol. 51: 977-983. https://doi.org/10.1099/00207713-51-3-977
  8. Ferris, M. J. and D. M. Ward. 1997. Seasonal distribution of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 63: 1375-1381.
  9. Hoeks, J. 1977. Mobility of Pollutants in Soil and Groundwater Near Waste Disposal Sites. Technical bulletin/Institute for Land and Water Management Research.
  10. Hong, S. H., K. C. Shin, and E. Y. Lee. 2010. Characterization of a nitrogen fixing bacteria Mycobacterium hominis sp. AKC-10. Kor. J. Microbiol. Biotechnol. 38: 302-307.
  11. Howard, G. T., B. Duos, J. Erin, and W. Horzelski. 2010. Characterization of the soil microbial community associated with the decomposition of a swine carcass. Int. Biodeter. Biodegrad. 64: 300-304. https://doi.org/10.1016/j.ibiod.2010.02.006
  12. Hu, J., D. Li, Q. Liu, Y. Tao, X. He, X. Wang, et al. 2009. Effect of organic carbon on nitrification efficiency and community composition of nitrifying biofilms. J. Environ. Sci. 21: 387-394. https://doi.org/10.1016/S1001-0742(08)62281-0
  13. Kim, M. J., T. H. Lee, H. W. Ryu, and K. S. Cho. 2003. Isolation of acetaldehyde-degrading bacteria, Comamonas sp. AAHD-A, and its degradation activity. Korean J. Odor Res. Eng. 11: 124-125.
  14. Leckie, S. E. 2005. Methods of microbial community profiling and their application to forest soils. Forest Ecol. Manag. 220: 88-106. https://doi.org/10.1016/j.foreco.2005.08.007
  15. Lee, E. H., H. Park, Y. S. Jo, H. W. Ryu, and K. S. Cho. 2010. Application of methodology for microbial community analysis to gas-phase biofilters. Korean Chem. Eng. Res. 48: 147-156.
  16. McGarvey, J. A., W. G. Miller, S. Sanchez, C. J. Silva, and L. C. Whitehand. 2005. Comparison of bacterial populations and chemical composition of dairy wastewater held in circulated and stagnant lagoons. J. Appl. Microbiol. 99: 867-877. https://doi.org/10.1111/j.1365-2672.2005.02662.x
  17. Ranjard, L., F. Poly, and S. Nazaret. 2000. Monitoring complex bacterial communities using culture-independent molecular techniques: Application to soil environment. Res. Microbiol. 151: 167-177. https://doi.org/10.1016/S0923-2508(00)00136-4
  18. Röling, W. F. M., B. M. Van Breukelen, M. Braster, and H. W. Van Verseveld. 2000. Linking microbial community structure to pollution: Biolog-substrate utilization in and near a landfill leachate plume. Wat. Sci. Technol. 41: 47-53.
  19. Sekiguchi, Y., H. Imachi, A. Susilorukmi, M. Muramatsu, A. Ohashi, H. Harada, et al. 2006. Tepidanaerobacter syntrophicus gen. nov., sp. nov., an anaerobic, moderately thermophilic, syntrophic alcohol- and lactate-degrading bacterium isolated from thermophilic digested sludges. Int. J. Syst. Evol. Microbiol. 56: 1621-1629. https://doi.org/10.1099/ijs.0.64112-0
  20. Sellers, R. F. 1995. Growth and titration of the viruses of footand mouth disease and vesicular stomatitis in kidney monolayer tissue cultures. Nature 176: 121-124.
  21. Sharma, P. K. and S. K. Das. 1984. Occurrence of foot-andmouth disease and distribution of virus type in the hill states of North Eastern region of India. Indian J. Anim. Sci. 4: 117-118.
  22. Tamilselvan, R. P., A. D. Sanyal, and B. Pattnaik. 2009. Genetic transitions of Indian serotype O Foot and Mouth Disease Virus isolates responsible for field outbreaks during 2001-2009: A brief note. OIE/FAO Reference laboratories network meeting: New Delhi, India, pp. 11-13.
  23. Tian, Y., H. Yang, X. Wu, and D. T. Li. 2005. Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater. J. Zhejiang Univ. Sci. 6: 165-170.
  24. Tibbett, M., D. O. Carter, T. Haslam, R. Major, and R. Haslam. 2004. A laboratory incubation method for determining the rate of microbiological degradation of skeletal muscle tissue in soil. J. Forensic Sci. 49: 560-565.
  25. Watanabe, K. and N. Hamamura. 2003. Molecular and physiological approaches to understanding the ecology of pollutant degradation. Curr. Opin. Biotechnol. 14: 289-295. https://doi.org/10.1016/S0958-1669(03)00059-4
  26. Woodbury, E. L. 1995. A review of the possible mechanisms for the persistence of foot-and-mouth disease virus. Epidemiol. Infect. 114: 1-13. https://doi.org/10.1017/S0950268800051864
  27. Zhang, D., X. Yuan, P. Guo, Y. Suo, X. Wang, W. Wang, and Z. Cui. 2011. Microbial population dynamics and changes in main nutrients during the acidification process of pig manures. J. Environ. Sci. 23: 497-505. https://doi.org/10.1016/S1001-0742(10)60434-2

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