Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Somatic Cell Nuclear Transfer in Rodents, the Little Big Animals

  • Roh, Sangho (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry)
  • Received : 2012.10.20
  • Accepted : 2012.11.05
  • Published : 2012.12.31
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Abstract

Transgenic rats and mice are useful experimental animal models for medical research including human disease model studies. Somatic cell nuclear transfer (SCNT) technology is successfully applied in most mammalian species including cattle, sheep, pig and mouse. SCNT is also considered to increase the efficacy of transgenic/knockout mouse and rat production. However, in the area of reproductive biotechnology, the rodent model is inadequate because of technical obstacles in manipulating the oocytes including intracytoplasmic sperm injection and SCNT. In particular, success of rat SCNT is very limited so far. In this review, the history of rodent cloning is described.

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References

  1. Booth PJ, Tan SJ, Holm P and Callesen H. 2001. Application of the zona-free manipulation technique to porcine somatic nuclear transfer. Cloning Stem Cells 3:191-197. https://doi.org/10.1089/15362300152725909
  2. Charreau B, Tesson L, Soulillou JP, Pourcel C and Anegon I. 1996. Transgenesis in rats: technical aspects and models. Transgenic Res. 5:223-234. https://doi.org/10.1007/BF01972876
  3. Du ZT, Roh S, Fida S, Handford C, Malakooti N, Morrison J and Trounson A. 2002. Postimplantation development of rat embryos cloned with neural stem cells. Theriogenology 57:410.
  4. Eggan K, Akutsu H, Loring J, Jackson-Grusby L, Klemm M, Rideout WM 3rd, Yanagimachi R and Jaenisch R. 2001. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc. Natl. Acad. Sci. U.S.A. 98:6209-6214. https://doi.org/10.1073/pnas.101118898
  5. Egli D, Rosains J, Birkhoff G and Eggan K. 2007. Developmental reprogramming after chromosome transfer into mitotic mouse zygotes. Nature 447:679-685. https://doi.org/10.1038/nature05879
  6. Enright BP, Kubota C, Yang X and Tian XC. 2003. Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin A or 5-aza-2'-deoxycytidine. Biol. Reprod. 69:896-901. https://doi.org/10.1095/biolreprod.103.017954
  7. Gao S, Chung YG, Williams JW, Riley J, Moley K and Latham KE. 2003. Somatic cell-like features of cloned mouse embryos prepared with cultured myoblast nuclei. Biol. Reprod. 69:48-56. https://doi.org/10.1095/biolreprod.102.014522
  8. Hayes E, Galea S, Verkuylen A, Pera M, Morrison J, Lacham- Kaplan O and Trounson AO. 2001. Nuclear transfer of adult and genetically modified fetal cells of the rat. Physiol. Genomics 5:193-203.
  9. Humpherys D, Eggan K, Akutsu H, Hochedlinger K, Rideout WM 3rd, Biniszkiewicz D, Yanagimachi R and Jaenisch R. 2001. Epigenetic instability in ES cells and cloned mice. Science 293:95-97. https://doi.org/10.1126/science.1061402
  10. Iannaccone P, Taborn G and Garton R. 2001. Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts. Zygote 9:135-143. https://doi.org/10.1017/S0967199401001149
  11. Jiang JY, Mizuno S, Mizutani E, Miyoshi K, Kimura N, Sasada H and Sato E. 2002. Nuclear transfer in rats using an established embryonic cell line and cumulus cells. J. Reprod. Dev. 48:505-511. https://doi.org/10.1262/jrd.48.505
  12. Kang H and Roh S. 2011. Extended exposure to trichostatin-A after activation improves in vitro development of somatic cell nuclear transfer murine embryos. J. Vet. Med. Sci. 73: 623-631. https://doi.org/10.1292/jvms.10-0492
  13. Kang H, Sung J and Roh S. 2011. Development of reversing the usual order of somatic cell nuclear transfer in mice. J. Emb. Trans. 26:85-89.
  14. Kang YK, Koo DB, Park JS, Choi YH, Chung AS, Lee KK and Han YM. 2001. Aberrant methylation of donor genome in cloned bovine embryos. Nat. Genet. 28:173-177. https://doi.org/10.1038/88903
  15. Kawamata M and Ochiya T. 2010. Generation of genetically modified rats from embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 107:14223-14228. https://doi.org/10.1073/pnas.1009582107
  16. Keefer CL and Schuetz AW. 1982. Spontaneous activation of ovulated rat oocytes during in vitro culture. J. Exp. Zool. 224:371-377. https://doi.org/10.1002/jez.1402240310
  17. Kishigami S, Mizutani E, Ohta H, Hikichi T, Thuan NV, Wakayama S, Bui HT and Wakayama T. 2006. Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem. Biophys. Res. Commun. 340:183-189. https://doi.org/10.1016/j.bbrc.2005.11.164
  18. Kishigawa H, Wakayama T and Yanagimachi R. 1999. Comparison of oocyte-activating agents for mouse cloning. Cloning 1:153-159. https://doi.org/10.1089/15204559950019915
  19. Kono T, Shinoda Y and Tsunoda Y. 1988. Nuclear transplantation of rat embryos. J. Exp. Zool. 248:303-305. https://doi.org/10.1002/jez.1402480308
  20. Kono T, Kwon OY and Nakahara T. 1991. Development of enucleated mouse oocytes reconstituted with embryonic nuclei. J. Reprod. Fertil. 93:165-172. https://doi.org/10.1530/jrf.0.0930165
  21. Kwon OY and Kono T. 1996. Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos. Proc. Natl. Acad. Sci. U.S.A. 93:13010-13013. https://doi.org/10.1073/pnas.93.23.13010
  22. Latham K E. 1999. Mechanisms and control of embryonic genome activation in mammalian embryos. Int. Rev. Cytol. 193:71-124.
  23. Liskovykh M, Chuykin I, Ranjan A, Safina D, Popova E, Tolkunova E, Mosienko V, Minina JM, Zhdanova NS, Mullins JJ, Bader M, Alenina N and Tomilin A. 2011. Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats. PLoS One 6:e27345. https://doi.org/10.1371/journal.pone.0027345
  24. Oback B, Wiersema AT, Gaynor P, Laible G, Tucker FC, Oliver JE, Miller AL, Troskie HE, Wilson KL, Forsyth JT, Berg MC, Cockrem K, McMillan V, Tervit HR and Wells DN. 2003. Cloned cattle derived from a novel zona-free embryo reconstruction system. Cloning Stem Cells 5:3-12. https://doi.org/10.1089/153623003321512111
  25. Ohgane J, Wakayama T, Senda S, Yamazaki Y, Inoue K, Ogura A, Marh J, Tanaka S, Yanagimachi R and Shiota K. 2004. The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice. Genes Cells 9:253-260. https://doi.org/10.1111/j.1356-9597.2004.00720.x
  26. Ogura A, Inoue K, Ogonuki N, Noguchi A, Takano K, Nagano R, Suzuki O, Lee J, Ishino F and Matsuda J. 2000. Production of male cloned mice from fresh, cultured, and cryopreserved immature Sertoli cells. Biol. Reprod. 62:1579-1584. https://doi.org/10.1095/biolreprod62.6.1579
  27. Ogura A, Ogonuki N, Takano K and Inoue K. 2001. Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics. Mamm. Genome 12:803-812. https://doi.org/10.1007/s00335-001-4007-5
  28. Ribas R, Oback B, Ritchie W, Chebotareva T, Ferrier P, Clarke C, Taylor J, Gallagher EJ, Mauricio AC, Sousa M and Wilmut I. 2005. Development of a zona-free method of nuclear transfer in the mouse. Cloning Stem Cells 7:126-138. https://doi.org/10.1089/clo.2005.7.126
  29. Rideout WM 3rd, Wakayama T, Wutz A, Eggan K, Jackson- Grusby L, Dausman J, Yanagimachi R and Jaenisch R. 2000. Generation of mice from wild-type and targeted ES cells by nuclear cloning. Nat. Genet. 24:109-110. https://doi.org/10.1038/72753
  30. Roh S. 2005. Effect of the timing of oocyte activation on development of rat somatic cell nuclear transfer embryos. Reprod. Dev. Biol. 29:229-234.
  31. Roh S. 2007. Effect of cytochalasin B in activation medium on the development of rat somatic cell nuclear transfer embryos. Reprod. Dev. Biol. 31:109-113.
  32. Roh S and Hwang WS. 2002. In vitro development of porcine parthenogenetic and cloned embryos: comparison of oocyteactivating techniques, various culture systems and nuclear transfer methods. Reprod. Fertil. Dev. 14:93-99. https://doi.org/10.1071/RD01090
  33. Roh S, Guo J, Malakooti N, Morrison JR, Trounson AO and Du ZT. 2003a. Birth of rats following nuclear exchange at the 2-cell stage. Zygote 11:317-321. https://doi.org/10.1017/S0967199403002375
  34. Roh S, Malakooti N, Morrison JR, Trounson AO and Du ZT. 2003b. Parthenogenetic activation of rat oocytes and their development in vitro. Reprod. Fertil. Dev. 15:135-140. https://doi.org/10.1071/RD02096
  35. Rybouchkim A and Dhont. 2000. Nuclear transfer into mouse oocytes by a conventional method of injection. Theriogenology 53:241.
  36. Rybouchkin A, Kato Y and Tsunoda Y. 2006. Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol. Reprod. 74:1083-1089. https://doi.org/10.1095/biolreprod.105.047456
  37. Szollosi D, Czolowska R, Soltynska MS and Tarkowski AK. 1986. Remodelling of thymocyte nuclei in activated mouse oocytes: An ultrastructural study. Eur. J. Cell. Biol. 42: 140-151.
  38. Takahashi K and Yamanaka S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663-676. https://doi.org/10.1016/j.cell.2006.07.024
  39. Tsunoda Y and Kato Y. 1997. Full-term development after transfer of nuclei from 4-cell and compacted morula stage embryos to enucleated oocytes in the mouse. J. Exp. Zool. 278:250-254. https://doi.org/10.1002/(SICI)1097-010X(19970701)278:4<250::AID-JEZ6>3.0.CO;2-O
  40. Tsunoda Y and Kato Y. 1998. Not only inner cell mass cell nuclei but also trophectoderm nuclei of mouse blastocysts have a developmental totipotency. J. Reprod. Fertil. 113: 181-184. https://doi.org/10.1530/jrf.0.1130181
  41. Vajta G, Lewis IM, Thouas GA and Trounson AO. 2001. Somatic cell cloning without micromanipulators. Cloning 3:89-95. https://doi.org/10.1089/15204550152475590
  42. Wakayama T and Yanagimachi R. 1999. Cloning the laboratory mouse. Semin. Cell Dev. Biol. 10:253-258. https://doi.org/10.1006/scdb.1998.0267
  43. Wakayama T and Yanagimachi R. 2001. Mouse cloning with nucleus donor cells of different age and type. Mol. Reprod. Dev. 58:376-383. https://doi.org/10.1002/1098-2795(20010401)58:4<376::AID-MRD4>3.0.CO;2-L
  44. Wakayama T, Perry ACF, Zuccotti M, Johnson KR and Yanagimachi R. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374. https://doi.org/10.1038/28615
  45. Wakayama T, Mombaerts P, Rodriguez I, Perry ACF and Yanagimachi R. 1999. Mice cloned from embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 96:14984-14989. https://doi.org/10.1073/pnas.96.26.14984
  46. Wakayama T, Shinkai Y, Tamashiro KL, Niida H, Blanchard DC, Blanchard RJ, Ogura A, Tanemura K, Tachibana M, Perry AC, Colgan DF, Mombaerts P and Yanagimachi R. 2000. Cloning of mice to six generations. Nature 407:318-319.
  47. Wells DN, Misica PM and Tervit HR. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60:996-1005. https://doi.org/10.1095/biolreprod60.4.996
  48. Wilmut I, Schieke AE, McWhir J, Kind AJ and Campbell KHS. 2007. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813.
  49. Zhou Q, Boulanger L and Renard JP. 2000. A simplified method for the reconstruction of fully competent mouse zygotes from adult somatic donor nuclei. Cloning 2:35-44. https://doi.org/10.1089/15204550050145111
  50. Zhou Q, Renard J-P, Le Friec G, Brochard V, Beaujean N, Cherifi Y, Fraichard A and Cozzi J. 2003. Generation of fertile cloned rats by regulating oocyte activation. Science 302:1179. https://doi.org/10.1126/science.1088313