한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제31권1호
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  • Pages.30-36
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  • 2012
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
권호 표지
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세포벽의 형태학적 변화와 ABC Transporter에 기초한 벼키다리병원균 Fusarium fujikuroi CF337의 살균제 prochloraz에 대한 저항성 반응

Morphological Changes of Fungal Cell Wall and ABC Transporter as Resistance Responses of Rice Bakanae Disease Pathogen Fusarium fujikuroi CF337 to Prochloraz

  • 양유리 (전남대학교 농업생명과학대학 친환경농업연구소) ;
  • 이시우 (국립농업과학원 농업생물부) ;
  • 이세원 (국립농업과학원 농업생물부) ;
  • 김인선 (전남대학교 농업생명과학대학 친환경농업연구소)
  • Yang, You-Ri (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Si-Woo (Division of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Se-Won (Division of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, In-Seon (Institute of Environmentally-friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 투고 : 2012.03.03
  • 심사 : 2012.03.21
  • 발행 : 2012.03.31
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초록

BACKGROUND: The resistance of rice bakanae disease pathogens against the fungicide prochloraz has been reported. Understanding the resistance mechanisms is an important for better control of the pathogens. In the present study, we investigated the resistance mechanisms of Fusarium fujikuroi CF337 (CF337) against prochloraz. METHODS AND RESULTS: Morphological changes in the cell wall of CF337 grown in potato dextrose broth (PDB) with or without prochloraz was investigated by transmission electron microscopy. Growth inhibition of CF337 was examined in PDB containing prochloraz or an ABC transporter inhibitor or both of them. Cell wall thickness of CF337 grown in PDB with prochloraz was significantly increased from $80.73{\pm}1.99nm$ to $193.11{\pm}7.07nm$. Significant inhibition in the growth of CF337 was observed in the presence of both prochloraz and the inhibitor, but no growth inhibition was observed in the presence of the inhibitor or prochloraz. Sequence analysis of ATP-binding cassette transporter (ABC) gene of CF337 showed 70 to 80% similarities to the genes of the pathogens resistant to other fungicides. CONCLUSION: Efflux transporter system and changes in cell wall thickness were suggested as resistance mechanisms of CF337 against prochloraz.

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참고문헌

  1. Albertson, G. D., Niimi, M., Cannon, R. D., Jenkinson, H. F., 1996. Multiple efflux mechanisms are involved in Candida albicans fluconazole resistance, Antimicrob. Agents Ch. 40, 2835-2841.
  2. Delye, C., Laigret, F., Corio-Costet, M. F., 1997. A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Appl. Environ. Microbiol. 63, 2966-2970.
  3. Elsztein, C., de Lucena, R. M., de Morais, M. A., Jr., 2011. The resistance of the yeast Saccharomyces cerevisiae to the biocide polyhexamethylene biguanide: involvement of cell wall integrity pathway and emerging role for YAP1. BMC Mol. Biol. 12, 1-10. https://doi.org/10.1186/1471-2199-12-1
  4. Hayashi, K., Schoonbeek, H., Waard, M. A. D.. 2002. Expression of the ABC transporter BcatrD from Botrytis cinerea reduces sensitivity to sterol demethylation inhibitor fungicides. Pestic. Biochem. Phys. 73, 110-121. https://doi.org/10.1016/S0048-3575(02)00015-9
  5. Henry, M. J., Sisler, H. D., 1984. Effects of sterol biosynthesis-inhibiting (SBI) fungicides on cytochrome P-450 oxygenations in fungi. Pestic. Biochem. Phys. 22, 262-275. https://doi.org/10.1016/0048-3575(84)90019-1
  6. Kim, I. S., Foght, J. M., Gray, M. R., 2002. Selective transport and accumulation of alkanes by Rhodococcus ertythropolis S+14He. Biotechnol. Bioengineer. 80, 650-659. https://doi.org/10.1002/bit.10421
  7. Kim, S. H., Park, M. R., Kim, Y. C., Lee, S. W., Choi, B. R., Lee, S. W., Kim, I. S., 2010. Degradation of prochloraz by rice Bakanae disease pathogen Fusarium fujikuroi with differing sensitivity: a possible explanation for resistance mechanism. J. Korean Soc. Appl. Biol. Chem. 53, 433-439. https://doi.org/10.3839/jksabc.2010.067
  8. Kim, S. K., Kim, Y. C., Lee, S., Kim, J. C., Yun, M. Y., Kim, I. S. 2011. Insecticidal activity of rhamnolipid isolated from Pseudomonas sp. EP-3 against green peach aphid (Myzue perscicae). J. Agric. Food Chem. 59, 934-938. https://doi.org/10.1021/jf104027x
  9. Lee, Y. H., Kim, S. Y., Choi, H. W., Lee, M. J., Ra, D. S., Kim, I. S., Park, J. W., Lee, S. W., 2010. Fungicide resistance of Fusarium fujikuroi isolated in Korea. Korean J. Pestic. Sci. 14, 427-432.
  10. Ma, Z., Proffer, T. J., Jacobs, J. L., Sundin, G. W., 2006. Overexpression of the 14alpha-demethylase target gene (CYP51) mediates fungicide resistance in Blumeriella jaapii. Appl. Environ. Microbiol. 72, 2581-2585. https://doi.org/10.1128/AEM.72.4.2581-2585.2006
  11. Ma, Z., Yoshimura, M. A., Michailides, T. J., 2003. Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchards in california. Appl. Environ. Microbiol. 69, 7145-7152. https://doi.org/10.1128/AEM.69.12.7145-7152.2003
  12. Mellado, E., Garcia-Effron, G., Alcazar-Fuoli, L., Melchers, W. J., Verweij, P. E., Cuenca-Estrella, M., Rodriguez- Tudela, J. L., 2007. A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations. Antimicrob. Agents Ch. 51, 1897-1904. https://doi.org/10.1128/AAC.01092-06
  13. Milano, A., Pasca, M. R., Provvedi, R., Lucarelli, A. P., Manina, G., de Jesus Lopes Ribeiro, A. L., Manganelli, R., Riccardi, G., 2009. Azole resistance in Mycobacterium tuberculosis is mediated by the MmpS5-MmpL5 efflux system. Tuberculosis 89, 84-90. https://doi.org/10.1016/j.tube.2008.08.003
  14. Moslem, M. A., Bahkali, A. H., Abd-Elsalam, K. A., Wit, P. J. G. M., 2010. An efficient method for DNA extraction from cladosporioid fungi. Genet. Mol. Res. 9, 2283-2291. https://doi.org/10.4238/vol9-4gmr936
  15. Parkinson, T., Falconer, D. J., Hitchcock, C. A., 1995. Fluconazole resistance due to energy-dependent drug efflux in Candida glabrata. Antimicrob. Agents Ch. 39, 1696-1699. https://doi.org/10.1128/AAC.39.8.1696
  16. Prudencio, C., Sansonetty, F., Sousa, M. J., Corte-Real, M., Leao, C., 2000. Rapid detection of efflux pumps and their relation with drug resistance in yeast cells. Cytometry. 39, 26-35. https://doi.org/10.1002/(SICI)1097-0320(20000101)39:1<26::AID-CYTO5>3.0.CO;2-C
  17. Sanglard, D., Ischer, F., Koymans, L., Bille, J., 1998. Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents. Antimicrob. Agents Ch. 42, 241-253. https://doi.org/10.1093/jac/42.2.241