Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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5'-CpG Island Promoter Hypermethylation of the CAV-1 Gene in Breast Cancer Patients of Kashmir

  • Syeed, Nidda (Dept of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Hussain, Firdous (Dept of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Husain, Syed Akhtar (Dept of Biosciences Jamia Millia Islamia) ;
  • Siddiqi, Mushtaq A. (Dept of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences)
  • Published : 2012.01.31
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Abstract

Background: Caveolin-1 (CAV-1), encoding the structural component of cellular caveolae, is a suggested tumor suppressor gene involved in cell signalling. Aberrant promoter methylation of CAV-1 is associated with inactivation of expression. We previously observed CAV-1 mutations in breast cancers and therefore devised this study to examine the hypermethylation status of the promoter region of CAV-1 with reference to breast cancer progression and development. Methods: Hypermethylation status of CAV-1 was analyzed by methylation specific PCR. Loss of expression of the CAV-1 gene was further evaluated by semi-quantitative rt-PCR. Results: 28/130 (21.5%) breast cancer cases showed promoter hypermethylation with reduced CAV-1 expression levels when compared with adjacent normal breast tissue. CAV-1 gene hypermethylation was significantly related to menopausal status, histopathological grade and age. Conclusion: The rationale of our study is that CAV-1 gene is transcriptionally repressed in breast cancer cells due to hypermethylation. Our results reveal that promoter hypermethylation and loss of expression of the CAV-1 gene is an important alternative mechanism for inactivation of CAV-1 leading to complete gene silencing.

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References

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