Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

  • Choi, Eun-A (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Yoon, Jeung-Won (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University) ;
  • Choi, Hak-Joo (Traditional and Biomedical Research Center, Daejeon University) ;
  • Kim, Dong-Hee (Traditional and Biomedical Research Center, Daejeon University) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University)
  • Received : 2011.09.21
  • Accepted : 2012.02.24
  • Published : 2012.03.31
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Abstract

Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). Results: IS4 concentration inhibited the production of interleukin-1beta (IL-$1{\beta}$), interleukin-6 (IL-6), and granulocyte-macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50 ${\mu}g/ml$) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF-${\alpha}$) stimulated by LPS. Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

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References

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