Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antioxidative Activity of Prunus sargentii Outer Bark Extractives

  • Park, Se-Yeong (Dept. of Forest Biometerials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Bae, Young-Soo (Dept. of Forest Biometerials Engineering, College of Forest and Environmental Sciences, Kangwon National University)
  • Received : 2012.03.14
  • Accepted : 2012.03.22
  • Published : 2012.03.25
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Abstract

The outer bark of Prunus sargentii was collected, air-dried and extracted with 70% aqueous acetone. Then it was successively partitioned with n-hexane, dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc) and $H_2O$. From the EtOAc soluble fraction, four compounds were isolated by the repeated Sephadex LH-20 column chromatography. The isolated compounds were determined as (+)-catechin (1), (-)-epicatechin (2), taxifolin (3), and neosakuranin (4) by the spectroscopic analysis including $^1H$, $^{13}C$-NMR, and 2D-NMR spectrometers. The antioxidative activities on the isolated compounds and the separated fractions were evaluated by DPPH radical scavenging assay. The crude, EtOAc, and $H_2O$ soluble fractions indicated good antioxidative potential compared to the $CH_2Cl_2$ and n-hexane soluble fractions.

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References

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