Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
권호 표지
DOI QR코드

DOI QR Code

Systematic study of Korean Asparagus L. based on morphology and nuclear ITS sequences

외부형태와 ITS 염기서열에 기초한 한국산 비짜루속 식물의 분류학적 고찰

  • Received : 2012.08.24
  • Accepted : 2012.09.14
  • Published : 2012.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Morphological and geographical examinations as well as phylogenetic analyses using ITS sequences were performed for Asparagus L. in Korea. A total of five species of Asparagus were confirmed to be distributed in South Korea. The shape of cladophylls, length of pedicels, and shape of perianth were considered to be important characteristics for the identification of Koran Asparagus species. A monophyly of each species was evident in the ITS phylogenetic trees in which multiple accessions (5 to 24, depending on species) represented each of the five Korean species. A. rigidulus Nakai, once considered conspecific to A. schoberioides Kunth, formed a distinct lineage in the ITS trees. Pedicels of A. rigidulus, which is distributed mainly in coastal areas, were about two times longer than those of A. schoberioides occurring in inland areas, suggesting that they should be treated as distinct taxa.

한국산 Asparagus L. (비짜루속)을 대상으로 외부형태 형질을 재검토하고 ITS 염기서열을 사용한 계통분석을 실시하였다. 외부형태를 분석한 결과 엽상경의 형태, 소화경의 길이, 화피의 형태 등이 종을 식별하는 데 중요한 형질이었으며, 남한에는 최근에 분포가 확인된 A. davuricus Fisch. and Link (망적천문동)을 포함하여 총 다섯 종의 Asparagus가 자생하는 것으로 확인되었다. 각 분류군별로 5 내지 24 개체를 포함하여 수행한 ITS 염기서열 계통분석에서 각 분류군은 모두 단계통군을 이루었다. 특히 A. schoberioides Kunth (비짜루)의 이명으로 처리되고 있는 A. rigidulus Nakai (노간주비짜루)는 A. shoberioides와 8개의 뉴클레오티드 자리에서 차이를 보이면서 독자적인 단계통군을 형성하였다. 독도, 제주도 및 중남부 해안에 주로 자라는 A. rigidulus의 소화경은 내륙에 분포하는 A. schoberioides에 비해 약 2배 길었다. 두 종의 형태적, 지리적, 계통 유전학적 차이는 A. rigidulus를 A. schoberioides와 다른 독립된 종으로 인정해야 함을 말해준다.

Keywords

References

  1. Alvarez, I. and J. F. Wendel. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution 29: 417-434. https://doi.org/10.1016/S1055-7903(03)00208-2
  2. Chen, X. and K. G. Tamaian. 2000. Asparagus L. In Flora of China Vol. 24. Wu, Z.-Y. and P. H. Raven (eds.), Science Press, Beijing. Pp. 208-213.
  3. Clifford, H. T. and J. G. Conran. 1987. Asparagaceae. In Flora of Australia. George, A. S. (ed.), Australian Government Publishing Service, Canberra. Pp. 159-164.
  4. Dellaporta, S. L. and A. Calderon-Urrea. 1933. Sex determination in flowering plants. The Plant Cell 5(10): 1241-1251.
  5. Feliner, G. N. and J. A. Rossell. 2007. Better the devil you know? Guidelines for insightful utilization of nrDNA ITS in specieslevel evolutionary studies in plants. Molecular Phylogenetics and Evolution 44: 911-919. https://doi.org/10.1016/j.ympev.2007.01.013
  6. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using bootstrap. Evolution 39: 783-791. https://doi.org/10.2307/2408678
  7. Fukuda, T., H. Ashizawa, R. Suzuki, T. Ochiai, T. Nakamura, A. Kanno, T. Kameya and J. Yokoyama. 2005. Molecular phylogeny of the genus Asparagus (Asparagaceae) inferred from plastid petB intron and petD-rpoA intergenic spacer sequences. Plant Species Biology 20: 121-132. https://doi.org/10.1111/j.1442-1984.2005.00131.x
  8. Galli, M. G., M. Bracale, A. Falavigna, F. Raffaldi, C. Savini and A. Vigo. 1993. Different kinds of male flowers in the dioecious plant Asparagus officinalis L. Sexual Plant Reproduction 6: 16-21.
  9. Il'in, M. M. 1968. Asparagus L. In Flora of U.S.S.R. Vol. 4. Komarov, V. L. (ed.), Bishen sigh Mahendra Pal Singh and Koeltz Scientific Books. Pp. 325-339.
  10. Im, R. J. 2000. Flora Coreana. The Science and Technology Publishing House. Pyongyang, Korea.
  11. Kim, Y.-I. and Y.-D. Kim. 2011. Molecular systematic study of Chrysosplenium series Pilosa (Saxifragaceae) in Korea. Journal of Plant Biology 54: 396-401. https://doi.org/10.1007/s12374-011-9175-6
  12. Kim, Y. S. and B. U. Oh. 1985. A systematic study on Liliaceae in Korea III. Karyology of genus Asparagus. Korean Journal of Plant Taxonomy 15: 1-12. (in Korean)
  13. Kim, Y. S. and W. T. Lee. 1985. A systematic study on Liliaceae in Korea IV. Anatomical study of genus Asparagus. Korean Journal of Plant Taxonomy 15: 13-24. (in Korean)
  14. Kimura, M. 1980. A simple method for estimating evolutionary rate of the base substitution through comparative studies of nucleotide sequence. Journal of Molecular Evolution 16: 111-120. https://doi.org/10.1007/BF01731581
  15. Kitagawa, M. 1939. Lineamenta Florae Manshuricae. Institute of Scientific Research. Manchoukuo. 3 (Appendix): 133-134.
  16. Komarov, V. L. 1901. Flora Manshuriae I. Acta Horti Petropolitani. 20: [I]-559.
  17. Kubitzki, K. and P. J. Rudall. 1998. Asparagaceae. In The Families and Genera of Vascular Plants. Kubitzki, K. (ed.), Berlin. 3: 125-129.
  18. Lee, N. S. 2007. Asparagus L. In The Genera of Vascular Plants of Korea. Park, C.-W. (ed.), Academy Publishing Co., Seoul, Korea. Pp. 1292-1293.
  19. Lee, T. B. 1979. Illustrated Flora of Korea. Hyangmunsa, Seoul, Korea. (in Korean)
  20. Lee, W. T. 1996. Lineamenta Florae Korea. Academy Press, Seoul, Korea. (in Korean)
  21. Lee Y. O., A. Kanno and T. Kameya. 1997. Phylogenetic relationships in the genus Asparagus based on the restriction enzyme analysis of the chloroplast DNA. Japanese Journal of Breeding 47: 375-378. https://doi.org/10.1270/jsbbs1951.47.375
  22. Maddison, D and W. Maddison. 2002. MacClade*. Analysis of Phylogeny and Character Evolution. 4.03 PPC ed. Sinauer Associates, Sunderland, Massachusetts, U.S.A.
  23. Makino, T. 1905. Observations on the flora of Japan. 5: 1-73.
  24. Nakai, T. 1913. De nonnulis Asparagis et Alliis Japonicis et Coreanis. Botanical Magazine (Tokyo) 27: 213-216. https://doi.org/10.15281/jplantres1887.27.323_213
  25. Nakai, T. 1923. Notulae ad plantas Japoniae et Koreae XXX. Botanical Magazine (Tokyo) 37: 69-82. https://doi.org/10.15281/jplantres1887.37.437_en69
  26. Noda, M. 1971. Flora of the North-Eastern province (Manshuria) of China. Kazama, Tokyo. Pp. 272-274.
  27. Ohwi, J. 1972. Flora of Japan. Tokyo. Pp. 1560.
  28. Okuyama, Y. and M. Kato. 2009. Unveiling cryptic species diversity of flowering plants: successful biological species identification of Asian Mitella using nuclear ribosomal DNA sequences. BMC Evolutionary Biology 9: 105. https://doi.org/10.1186/1471-2148-9-105
  29. Palibin, J. 1901. Conspectus Florae Koreae IV. Acta Horti Petropolitani 19: 101-151.
  30. Stajner, N., B. Bohanec and B. Javornik. 2002. Genetic variability of economically important Asparagus species as revealed by genome size analysis and rDNA ITS polymorphisms. Plant Science 162: 931-937. https://doi.org/10.1016/S0168-9452(02)00039-0
  31. Swofford, D. L. 2001. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Method) ver.4.0b10. Sinauer Associates, Sunderland, Massachusetts.
  32. Thompson J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, D. G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  33. Yabe, Y. 1903. Liliaceae Koreae Uchiyamanae. Botanical Magazine (Tokyo) 17: 133-136.

Cited by

  1. The study of distribution characteristics of vascular and naturalized plants in Dokdo, South Korea vol.7, pp.2, 2014, https://doi.org/10.1016/j.japb.2014.03.011
  2. An unexpected genetic diversity pattern and a complex demographic history of a rare medicinal herb, Chinese asparagus (Asparagus cochinchinensis) in Korea vol.9, pp.None, 2012, https://doi.org/10.1038/s41598-019-46275-9