생명과학회지 (Journal of Life Science)

  • 제22권3호
  • /
  • Pages.340-346
  • /
  • 2012
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

새로운 생균제 CS61 배양액의 사료 내 급여가 육계의 생산성에 미치는 영향

Effects of Dietary Supplementation of a New Probiotic CS61 Culture on Performance in Broiler Chickens

  • Kim, Sung-Hwan (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, In-Chul (College of Veterinary Medicine, Chonnam National University) ;
  • Baek, Hyung-Seon (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Seong-Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Hyoung-Chin (Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Jin-Cheol (College of Pharmacy, Chosun University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University)
  • 투고 : 2011.11.01
  • 심사 : 2012.03.02
  • 발행 : 2012.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

가금류 제품에서 항생제의 잔류와 내성균의 출현으로 인해 항생제 대체물질에 대한 연구가 활발히 진행되고 있으며, 특히 생균제와 활성촉진제 또는 이들을 조합한 신바이오틱의 사용이 권장되고 있다. 본 연구는 새로운 생균제 CS61 배양액의 사료첨가 급여가 육계의 성장 및 사료효율에 미치는 영향과 안전성을 평가하여 항생제를 대체할 수 있는 사료첨가제로서의 개발가능성을 알아보기 위해 수행하였다. CS61 배양액은 0, 0.1 및 1%의 용량으로 28일간 사료에 혼합하여 육계에게 급여하였다. 시험결과, 시험물질 처치군에서 부검 시의 체중과 일당증체량이 대조군에 비해 용량의존적으로 증가하였다. CS61 배양액의 사료 내 첨가는 대조군 동물에 비해 사료효율도 개선하는 것으로 나타났다. 반면, 일반증상과 사망률, 부검소견, 혈액학치 및 혈청생화학치에서는 시험물질의 처치와 관련된 독성소견이 관찰되지 않았다. RAW 264.7 세포를 이용한 일산화질소 시험에서 정제된 CS61 펩타이드는 lipopolysaccharide에 유도된 일산화질소 생성을 용량의존적으로 억제하였다. 본 시험결과는 육계에 CS61 배양액의 사료첨가 급여는 항염증효과를 통해 성장과 사료효율을 개선할 수 있음을 보여주며, 사료첨가제로서 CS61 배양액의 유용성과 개발가능성을 시사해 주고 있다.

Bacterial resistance to antibiotics and residues of antibiotics in poultry products have encouraged the use of probiotics, prebiotic substrates, and synbiotic combinations of prebiotics and probiotics as alternative approaches to the use of antibiotics in poultry. The present study was carried out to evaluate the effect of a new probiotic CS61 culture on growth performance, feed conversion efficiency, and safety in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Two dosages of CS61 culture (0.1% and 1%) were fed to chickens for 28 days. The results showed that terminal body weight and daily weight gain in the treatment groups increased in a dose-dependent manner when compared with the control group. Dietary supplementation with CS61 culture also improved feed conversion rate compared to the control group. There were no treatment-related toxic effects in terms of clinical findings, mortality, necropsy findings, hematology, or serum biochemistry parameters in any group tested. The nitric oxide assay showed that CS61 peptide has a dose-dependent inhibitory effect on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. The results of this experiment indicated that dietary supplementation of CS61 culture may improve growth performance and feed conversion efficiency in chickens through its anti-inflammatory effect.

키워드

참고문헌

  1. CDC (Centers for Disease Control and Prevention). 2011. Foodborne Illness. Atlanta. Available at: http://www.cdc.gov/ncidod/dbmd/diseaseinfo/foodborneinfections_t.htm (accessed 05 Oct 2011).
  2. Chaucheyras-Durand, F. and H. Durand. 2010. Probiotics in animal nutrition and health. Benef. Microbes 1, 3-9. https://doi.org/10.3920/BM2008.1002
  3. Fuller, F. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66, 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  4. Gaggìa, F., P. Mattarelli, and B. Biavati. 2010. Probiotics and prebiotics in animal feeding for safe food production. Int. J. Food Microbiol. 141, S15-28. https://doi.org/10.1016/j.ijfoodmicro.2010.02.031
  5. Higa, T. and J. F. Parr. 1994. Beneficial and effective microorganisms. In: International Nature Farming Research Center. Atami, Japan.
  6. Kim, J. D., S. J. Auel, and K. S. Shim. 2010. Effects of feed additives as an alternative for antibiotics on growth performance and feed cost in growing-finishing pigs. Korean J. Org. Agric. 18, 233-244.
  7. Kim, J. H., Y. M. Kim, S. C. Kim, M. H. Ha, Y. D. Ko, and C. H. Kim. 2001. Effect of dietary supplementation of probiotics ($Economix^{(R)}$) on the performance of broiler chicks and noxious gas reduction in a broiler house. J. Anim. Sci. Technol. 43, 349-360.
  8. Kim, S. H., I. C. Lee, S. S. Kang, C. Moon, S. H. Kim, D. H. Shin, H. C. Kim, J. C. Yoo, and J. C. Kim. 2011. Effects of bamboo charcoal and bamboo leaf supplementation on performance and meat quality in chickens. J. Life Sci. 21, 805-810. https://doi.org/10.5352/JLS.2011.21.6.805
  9. Kim, Y. R., B. K. Ahn, M. S. Kim, and C. W. Kang. 2000. Effects of dietary supplementation of probiotics ($MS^{102(R)}$) on performance, blood cholesterol level, size of small intestine and intestinal microflora in broiler chicks. J. Anim. Sci. Technol. 42, 849-858.
  10. Ko, Y. D., J. H. Sin, S. C. Kim, Y. M. Kim, K. D. Park, and J. H. Kim. 2003. Effects of dietary probiotic on performance, noxious gas emission and microflora population on the cecum in broiler. J. Anim. Sci. Technol. 45, 559-568. https://doi.org/10.5187/JAST.2003.45.4.559
  11. Korhonen, R., A. Lahti, H. Kankaanranta, and E. Moilanen. 2005. Nitric oxide production and signaling in inflammation. Curr. Drug Targets Inflamm. Allergy 4, 471-479. https://doi.org/10.2174/1568010054526359
  12. Lin, D. C. 2003. Probiotics as functional foods. Nutr. Clin. Pract. 18, 497-506. https://doi.org/10.1177/0115426503018006497
  13. Lutful Kabir, S. M. 2009. The role of probiotics in the poultry industry. Int. J. Mol. Sci. 10, 3531-546. https://doi.org/10.3390/ijms10083531
  14. NVRQS (National Veterinary Research and Quarantine Service). 2008. Guidelines on animal medical supplies for formula feed production. Public Notice No. 2008-25, Anyang, Korea.
  15. Park, S. H., J. S. Choi, D. S. Jung, J. H. Auh, and Y. I. Choi. 2010. Effects of complex probiotics and antibiotics on growth performance and meat quality in broilers. Korean J. Food Sci. Ani. Resour. 30, 504-511. https://doi.org/10.5851/kosfa.2010.30.3.504
  16. Shin, H., S. Lee, J. S. Kim, J. Kim, and K. H. Han. 2010. Socioeconomic costs of food-borne disease using the cost-of-illness model: applying the QALY method. J. Prevent. Med. Pub. Health 43, 352-361. https://doi.org/10.3961/jpmph.2010.43.4.352
  17. Stamlar, J. S., D. J. Singel, and J. Loscalzo. 1992. Biochemistry of nitric oxide and its redox-activated forms. Science 258, 1898-1902. https://doi.org/10.1126/science.1281928
  18. Talebi, A., B. Amirzadeh, B. Mokhtari, and H. Gahri. 2008. Effects of a multi-strain probiotic (PrimaLac) on performance and antibody responses to Newcastle disease virus and infectious bursal disease virus vaccination in broiler chickens. Avian Pathol. 37, 509-512. https://doi.org/10.1080/03079450802356995
  19. Yan, F. and D. B. Polk. 2010. Probiotics: progress toward novel therapies for intestinal diseases. Curr. Opin. Gastroenterol. 26, 95-101. https://doi.org/10.1097/MOG.0b013e328335239a
  20. Yang, Y. S., T. H. Ahn, J. C. Lee, C. Moon, S. H. Kim, W. Jun, S. C. Park, H. C. Kim, and J. C. Kim. 2008: Protective effects of $Pycnogenol^{(R)}$ on carbon tetrachloride-induced hepatotoxicity in Sprague-Dawley rats. Food Chem. Toxicol. 46, 380-387. https://doi.org/10.1016/j.fct.2007.08.016
  21. Yu, D. J., J. C. Na, T. H. Kim, S. H. Kim, and S. J. Lee. 2004. Effect of supplementation of complex probiotics on performances, physico-chemical properties of meat and intestinal microflora in broiler. J. Anim. Sci. Technol. 46, 593-602. https://doi.org/10.5187/JAST.2004.46.4.593

피인용 문헌

  1. Feed additives in broiler diets to produce healthy chickens without in-feed antimicrobial compounds vol.41, pp.4, 2014, https://doi.org/10.7744/cnujas.2014.41.4.441