Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Doinsenggitang on Melanin Synthesis and Gene Expression Inhibition in B16F10 Melanoma Cells

도인승기탕의 B16F10 세포주에서의 멜라닌 생성 및 유전자 발현 억제 효과

  • Hwang, Ju-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Hui-Jung (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Hwang, Eun-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Tae-Soon (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Son, Jun-Ho (Korea Promotion Institute for Traditional Medicine Industry)
  • Received : 2011.10.11
  • Accepted : 2012.02.16
  • Published : 2012.03.30
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Abstract

This study involved observation of the inhibitory effect of 70% EtOH and water extracts from Doinseunggitang on melanin synthesis, tyrosinase activity, and western blotting using B16F10 melanoma cells. Doinseunggitang extracts inhibited melanin synthesis and tyrosinase activity in a dependent manner. As a result, it was found that Doinseunggitang 70% EtOH extracts inhibit melanin synthesis and tyrosinase activity, respectively, by 40% and 51%. In addition, western blotting analysis showed that 70% EtOH extracts inhibited tyrosinase, MITF, TRP-1, and TRP-2 expression. These results show that 70% ethanol extracts of Doinseunggitang could be developed as a skin whitening material in cosmetics.

본 연구는 B16F10 melanoma 세포를 사용하여 도인승기탕의 70% EtOH와 물 추출물의 멜라닌 생합성, tyrosinase 활성, western blotting으로 측정하였다. 도인승기탕 추출물은 농도 의존적으로 멜라닌 생합성과 tyrosinase활성을 저해하였다. 그 결과 도인승기탕 70% 에탄올 추출물이 멜라닌 합성을 40%, tyrosinase는 51% 저해효과를 나타내었다. Western blot을 이용하여 B16F10 melanoma 세포 내에 tyrosinase, TRP-1, TRP-2, MITF의 발현을 억제하는 효과를 관찰하였다. 이상의 결과에 따라 도인승기탕의 70% 에탄올 추출물은 미백 소재로서 가능성을 가지는 것으로 나타났다.

Keywords

References

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