Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Promotion of in vitro shoot proliferation in rose by addition of liquid medium to culture

액체배지 첨가에 의한 장미 기내 신초 증식 촉진

  • Lee, Ye Ji (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Jung Lim (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyung, Nam-In (Department of Plant Science and Technology, Sangmyung University) ;
  • Kim, Seung Tae (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Eun Kyung (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kwon, O Hyeon (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Won Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Su Young (National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 이예지 (농촌진흥청 국립원예특작과학원) ;
  • 이정림 (농촌진흥청 국립원예특작과학원) ;
  • 형남인 (상명대학교 식물식품공학과) ;
  • 김성태 (농촌진흥청 국립원예특작과학원) ;
  • 이은경 (농촌진흥청 국립원예특작과학원) ;
  • 권오현 (농촌진흥청 국립원예특작과학원) ;
  • 김원희 (농촌진흥청 국립원예특작과학원) ;
  • 이수영 (농촌진흥청 국립원예특작과학원)
  • Received : 2012.11.26
  • Accepted : 2012.12.05
  • Published : 2012.12.31
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Abstract

To promote the growth and proliferation of in vitro rose (Rosa hybrida L) shoots, a liquid medium was added to shoot culture. Shoots were obtained by culturing internodes of four cultivars, 'Antique Curl', 'Shiny Orange', 'White Zen', and 'Red Zen', and then were proliferated by the subculture two times. An addition with 10~15 mL of liquid medium enhanced the shoot elongation of all four cultivars. However, the effect of liquid medium addition to culture of in vitro shoot for proliferation was dependent on cultivars of rose.

장미 기내 신초 생육을 촉진시키고자 액체배지 첨가 실험을 실시하였다. 국립원예특작과학원 육성 신품종 '엔텍컬', '샤이니오렌지', '화이트젠', '레드젠' 등 4품종의 internode를 기내 도입한 후, 2회의 계대배양에 의해 신초를 증식하였다. 10~15 mL의 액체배지 첨가에 의해 4품종 모두 신초의 신장이 촉진되었고, 증식력에 있어서는 품종간 차이를 보였다.

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References

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