Journal of Yeungnam Medical Science

Yeungnam University College of Medicine, Yeungnam University Institute Medical Science (영남대 의대 학술지 편집위원회)
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Usefulness of the BACTEC MGIT 960 System for Mycobacterial Culture and TB Ag MPT64 Immunochromatographic Assay to Identify Mycobacterium tuberculosis

항산균 배양에서 BACTEC MGIT 960 System의 유용성 및 MGIT 양성배지에서 결핵균 진단을 위한 TB Ag MPT64 면역발색법의 유용성

  • Lee, Seung Hun (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Lee, Min Jeong (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Lee, Jeong-Mi (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Yim, Su Jin (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Lee, Seung Jun (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Kim, You Eun (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Cho, Yu Ji (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Jeong, Yi Yeong (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Kim, Ho Cheol (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Lee, Jong Deog (Department of Internal Medicine, School of Medicine, Gyeongsang National University) ;
  • Kim, Sun Joo (Department of Laboratory Medicine, School of Medicine, Gyeongsang National University) ;
  • Hwang, Young Sil (Department of Internal Medicine, School of Medicine, Gyeongsang National University)
  • 이승훈 (경상대학교 의학전문대학원 내과학교실) ;
  • 이민정 (경상대학교 의학전문대학원 내과학교실) ;
  • 이정미 (경상대학교 의학전문대학원 내과학교실) ;
  • 임수진 (경상대학교 의학전문대학원 내과학교실) ;
  • 이승준 (경상대학교 의학전문대학원 내과학교실) ;
  • 김유은 (경상대학교 의학전문대학원 내과학교실) ;
  • 조유지 (경상대학교 의학전문대학원 내과학교실) ;
  • 정이영 (경상대학교 의학전문대학원 내과학교실) ;
  • 김호철 (경상대학교 의학전문대학원 내과학교실) ;
  • 이종덕 (경상대학교 의학전문대학원 내과학교실) ;
  • 김선주 (경상대학교 의학전문대학원 진단검사의학교실) ;
  • 황영실 (경상대학교 의학전문대학원 내과학교실)
  • Received : 2012.08.28
  • Accepted : 2012.10.19
  • Published : 2012.12.31
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Abstract

Background: This study was conducted to evaluate the usefulness of the BACTEC MGIT (Mycobacterium Growth Indicator Tube) 960 system for mycobacteria culture and immunochromatographic assay to identify Mycobacterium tuberculosis (MTB) in positive MGIT culture. Methods: Mycobacteria-culture-positive cases were retrospectively analyzed from December 2010 to July 2011. The detection rates and the recovery times of the mycobacteria between the Ogawa media and the MGIT were compared. An immunochromatographic assay (ICA) (SD BIO-LINE) was also performed in the positive MGIT culture for identification, and the results were compared with those of the Ogawa media in the Korea National Tuberculosis Association. Results: Among the 261 patients (M:F, 168:93; mean age, $61.6{\pm}17.16$ yrs), 450 specimens (sputa, 365; bronchial washing, 61; and pleural effusion, 24) were found positive with mycobacteria. Mycobacteria were grown both on the MGIT and Ogawa media in 310 cases (68.9%); only on the MGIT in 115 cases (22.6%); and only on the Ogawa media in 25 cases (5.5%) (p<0.05). The recovery time was $28.2{\pm}8.9$ days in the Ogawa media and $11.1{\pm}5.8$ days in the MGIT (p<0.05). Among the 127 cases from the positive MGIT culture, all 92 cases that were confirmed as MTB cases bythe Korea National Tuberculosis Association were identified as MTB by ICA, with 100% sensitivity. Conclusion: MGIT increases the detection rate and shortens the recovery time of mycobacteria in clinical respiratory specimens, and the TB Ag MPT64 kit using ICA is useful in identifying MTB in a positive MGIT culture.

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