Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Extracellular Prostaglandin $E_2$ Upregulation Effect of the Methanol Extract of Artemisia argyi

  • Lee, Kyoung In (Biotechnology Industrialization Center, Dongshin University) ;
  • Moon, Young Sook (Research Center for Resistant Cells, Chosun University) ;
  • Pyo, Byoung Sik (Department of Oriental Medicine Materials, Dongshin University) ;
  • Choi, Chul Hee (Research Center for Resistant Cells, Chosun University)
  • Received : 2012.08.29
  • Accepted : 2012.09.21
  • Published : 2012.12.31
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Abstract

Since 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is the key metabolic enzyme of prostaglandin $E_2$ ($PGE_2$), inhibition of 15-PGDH is supposed to facilitate various physiological functions by increasing $PGE_2$. Methanol extract of Artemisia argyi (AAME) inhibited 15-PGDH ($IC_{50}$: $13.13{\mu}g/mL$) with relatively low cytotoxicity ($IC_{50}$: $415.00{\mu}g/mL$) and elevated extracellular $PGE_2$ levels in HaCaT cells. Real-time PCR analysis showed that AAME decreased significantly mRNA expression of PG transporter (PGT) in HaCaT cells. These results indicate that AAME could be applicable to functional materials as a 15-PGDH inhibitor and PGT expression inhibitor for the upregulation of extracellular $PGE_2$ level.

Keywords

References

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