Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Probiotic Potential of Lactobacillus Isolates

Lactobacillus 분리균주의 프로바이오틱스로서의 가능성 검토

  • Bang, Ji-Hun (Department of Biology, Changwon National University) ;
  • Shin, Hwa-Jin (Department of Biology, Changwon National University) ;
  • Choi, Hye-Jung (Department of Biology and Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Ahn, Cheol-Soo (Cho-A Pharm. Co, LTD.) ;
  • Jeong, Young-Kee (Department of Biotechnology, Dong-A University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University)
  • Received : 2012.02.19
  • Accepted : 2012.02.18
  • Published : 2012.02.28
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Abstract

The purpose of this study was to investigate the probiotic properties of lactic acid bacterial strains isolated from animal feces. BCNU 9041 and BCNU 9042 isolates were assigned to Lactobacillus brevis on the basis of their physiological properties and 16S ribosomal DNA sequences analysis. They were confirmed as safe bioresources because of their non-hemolytic activities and non-production of harmful ${\beta}$-glucosidase, ${\beta}$-glucuronidase, tryptophanase, or urease. These isolates were also highly resistant to acid (at pH 2.5) and bile acids (at concentration of 0.3%, 0.6%, and 1% oxgall). In addition, they exhibited good antibacterial activity against food-borne bacteria, especially Bacillus cereus, Listeria monocytogenes, and Shigella sonnei. Furthermore, it was demonstrated that they have the highest levels of hydrophobicity and that they showed bile salt hydrolytic and cholesterol assimilation activity. These results suggest that BCNU 9041 and 9042 have good potential for application in functional foods and health-related products.

본 연구는 동물의 분변으로부터 분리한 유산균의 프로바이오틱 특성을 조사할 목적으로 시행되었다. 생리생화학적 특성과 16S 리보솜 DNA 분석 결과 BCNU 9041, 9042는 Lactobacillus brevis와 99%의 상동성을 나타내었다. 이들 균주를 대상으로 기초적인 안전성 실험을 시행한 결과, 이들 균주는 용혈현상이 나타나지 않으며 ${\beta}$-glucosidase, ${\beta}$-glucuronidase, tryptophanase 및 urease와 같은 유해한 생성물을 생성하지 않는 안전한 생물자 원임이 확인되었다. BCNU 9041 및 9042은 pH 2.5의 산성 조건 및 담즙에서(0.3, 0.6, 1%의 oxgall이 포함된 MRS broth) 높은 생존률을 나타내었다. 뿐만 아니라 식중독 원인 세균에 대하여 항균활성을 가지고 있으며, 특히 Bacillus cereus, Listeria monocytogenes 및 Shigella sonnei 에 대한 항균활성이 뛰어났다. 또한 BCNU 9041, BCNU 9042은 92-95%의 높은 소수성과 BSH (bile salt hydrolytic) 활성 및 cholesterol 흡수력이 우수함이 확인되었다. 이들 결과를 바탕으로 프로바이오틱로서의 우수한 기능성을 가진 BCNU 9041와 BCNU 9042의 기능성 식품 및 건강관련 제품으로의 활용이 기대된다.

Keywords

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