생명과학회지 (Journal of Life Science)

  • 제22권2호
  • /
  • Pages.226-231
  • /
  • 2012
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Cordycepin 고함유 동충하초 수용성 추출물의 이화학적 특성과 혈전 용해활성

Fibrinolytic Activity and Chemical Properties of Cordycepin-Enriched Cordyceps militaris JLM 0636

  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 이재홍 (동아대학교 대학원 의생명과학과) ;
  • 강민정 (동아대학교 대학원 의생명과학과) ;
  • 차재영 (대선주조(주) 기술연구소) ;
  • 조영수 (동아대학교 생명공학과)
  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Lee, Jae-Hong (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Kang, Min-Jeong (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 투고 : 2011.12.16
  • 심사 : 2012.02.10
  • 발행 : 2012.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cordycepin 고함유 번데기 동충하초(Cordyceps militaris JLM0636, $CM{\alpha}$)와 일반 번데기 동충하초(Cordyceps militaris, CM)의 생리활성 물질 및 영양성분 분석과 혈전 용해 활성을 비교 검토하였다. Total polyphenol compound, flavonoids 및 glutathione 함량은 CM 보다 $CM{\alpha}$에서 높은 함량을 보였다. CM 및 $CM{\alpha}$의 주요 미네랄 구성 성분으로는 K, Ca, Mg, Na이고, 주요 지방산 조성은 linolenic acid, linoleic acid, oleic acid, palmitic acid으로 나타났다. 혈전 용해 활성을 비교 검토한 결과 $CM{\alpha}$이 CM보다 높은 활성을 보였다. 이상의 실험 결과 $CM{\alpha}$의 생리활성물질과 혈전 용해 활성을 이용한 제품 개발에 필요한 기초 자료를 제공하고 유용하게 사용될 수 있을 것으로 사료되어 진다.

The effect of cordycepin-enriched Cordyceps militaris JLM 0636 ($CM{\alpha}$) and Cordyceps militaris (CM) on fibrinolytic activity was investigated. The bioactive compounds and nutritional materials such as polyphenolic compounds, flavonoids, glutathione, minerals, and fatty acids were also measured. Concentrations of polyphenol compounds, flavonoids, and glutathione were higher in $CM{\alpha}$ than that in CM. The major minerals of both materials were K, Ca, Mg, and Na. The major fatty acids of both materials were linolenic acid, linoleic acid, oleic acid, and palmitic acid. Fibrinolytic activity was higher in $CM{\alpha}$ than that in CM. These results may provide the basic data to understand the fibrinolytic activity and bioactive compounds of $CM{\alpha}$.

키워드

참고문헌

  1. A.O.A.C. 1975. Official methods of analysis. 12th eds., Association of official analytical chemists. Washington, D.C., U.S.A.
  2. Astrup, T. and S. Mullertz. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351. https://doi.org/10.1016/0003-9861(52)90121-5
  3. Butenas, S. and S. Mullertz. 2002. Blood coagulation. Biochemistry (Moscow) 67, 3-12. https://doi.org/10.1023/A:1013985911759
  4. Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136.
  5. Cha, J. Y., B. K. Park, K. E. Eom, H. Y. Ahn, and Y. S. Cho. 2009. Effect of Glutathione-Enriched Saccharomyces cerevisiae FF-8 on Tissues Lipid Peroxidation in Orotic Acid-Induced Fatty Liver Model Rats. J. Life Sci. 19, 322-326. https://doi.org/10.5352/JLS.2009.19.3.322
  6. Cha, J. Y., H. Y. Ahn, S. J. Heo, M. J. Kang, J. H. Lee, S. H. Park, Y. K. Jeong, and Y. S. Cho. 2011. Improvement effect of cordycepin-enriched Cordyceps militaris JLM 0636 powder against orotic acid-induced fatty liver in rats. J. Life Sci. 21, 1274-1280. https://doi.org/10.5352/JLS.2011.21.9.1274
  7. Cha, J. Y., H. I. Moon, H. Y. Ahn, S. J. Heo, and Y. S. Cho. 2011. Comparative effects of Paecilomyces japonic, Cordyceps militaris, cordycepin3 enriched Cordyceps militaris against antioxidant and hypolipidemia in alcohol feeding Sprague-Dawley rats. Immunopharmacology & Immunotoxicology 2011 in press.
  8. Cho, H. J., J. Y. Cho, M. H. Rhee, H. S. Kim, H. S. Lee, and H. J. Park. 2007. Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin. J. Microbiol. Biotechnol. 17, 1134-1138.
  9. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42. https://doi.org/10.1002/bimj.19590010102
  10. Eikelboom, J. W., S. L. Zelenkofske, and C. P. Rusconi. 2010. Coagulation factor IXa as a target for treatment and prophylaxis of venous thromboembolism. Arterioscler. Thromb. Vasc. Biol. 30, 382-387. https://doi.org/10.1161/ATVBAHA.110.203117
  11. Fahey, R. C. and G. I. Newton. 1983. Eutamoeba historlytica: A Eukaryote without glutathione metabolism. Science 224, 70-72. https://doi.org/10.1126/science.6322306
  12. Guo, P., Q. Kai, J. Gao, Z. Q. Lian, C. M. Wu, C. A. Wu, and H. B. Zhu. 2010. Cordycepin prevents hyperlipidemia in hamsters fed a high-fat diet via activation of AMP-activated protein kinase. J. Pharmacol. Sci. 113, 395-403. https://doi.org/10.1254/jphs.10041FP
  13. Han, E. K., Y. X. Jin, Y. S. Yoo, E. J. Jung, J. Y. Lee, and C. K. Chung. 2009. Effect of Artemisia capillaris and Paecilomyces japonica on the Reduction of Hepatotoxicity and Lipid Metabolism Induced by Ethanol. J. Korean Soc. Food Sci. Nutr. 38, 1016-1023. https://doi.org/10.3746/jkfn.2009.38.8.1016
  14. Ji, D. B, J. Ye, C. L. Li, Y. H. Wang, J. Zhao, and S. Q. Cai. 2009. Antiaging effect of Cordyceps sinensis extract. Phytother. Res. 23, 116-122. https://doi.org/10.1002/ptr.2576
  15. Jia, Z., M. Tang, and J. Wu. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem. 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  16. Kenzo, Y., T. Hirashi, and H. Yoshiteru. 1985. Glutathione no seizouho. Japan Patent 60-160894.
  17. Kim, H. K., Y. J. Choi, and K. H. Kim. 2002. Functional activities of microwave-assisted extracts from Flammulina velutipes. Korean J. Food Sci. Technol. 34, 1013-1017.
  18. Kim, H. K., Y. J. Choi, S. W. Jeong, K. H. Kim. 2002. Functional activities of microwave-assisted extracts from Lyophyllum ulmarium. Korean J. Food Preserv 9, 385-390.
  19. Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338.
  20. Li, S. P., G. H. Zhang, Q. Zeng, Z. G. Huang, Y. T. Wang, T. T. Dong, and K. W. Tsim. 2006. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 13, 428-433. https://doi.org/10.1016/j.phymed.2005.02.002
  21. Lie, C. H., M. T. Huang, and P. C. Huang. 1995. Sources of triglyceride accumulation in liver of rats fed a cholesterol-supplemented diet. Lipids 30, 527-532. https://doi.org/10.1007/BF02537027
  22. Muller, W. E., B. E. Weiler, R. Charubala, W. Pfleiderer, L. Leserman, R. W. Sobol, R. J. Suhadolnik, and H. C. Schroder. 1991. Cordycepin analogues of 2', 5'-oligo-adenylate inhibition of reverse transcriptase. Biochemistry 30, 2027-2033. https://doi.org/10.1021/bi00222a004
  23. Murata, K. and A. Kimura. 1986. Relationship between glutathione contents and generation times in Saccaromyces cerevisiae. Agric. Biol. Chem. 50, 1055-1056. https://doi.org/10.1271/bbb1961.50.1055
  24. Ok, M. and Y. S. Cho. 2005. Screening of fibrinolytic enzyme producing from microorganism in Korean fermented soybean paste and optimum conditions of enzyme production. Korean J. Food Preserv 12, 643-649.
  25. Owens, C. W. I. and R. V. Belcher. 1965. A colorimetric micro-method for the determination of glutathione. Biochem. J. 94, 705-711.
  26. Sohn, H. Y., Y. S. Kwon, Y. S. Kim, H. Y. Kwon, G. S. Kwon, G. J. Kim, C. S. Kwon, and K. H. Son. 2004. Screening of thrombin inhibitors from medicinal and wild plants. Korean J. Pharmacogn. 35, 52-61.
  27. Sugimura, Y. and K. Yamamoto. 1998. The protective effect of glutathione-enriched yeast extract on acetaminophen-induced liver damage in rats. J. Jpn. Soc. Nutr. Food Sci. 51, 189-193. https://doi.org/10.4327/jsnfs.51.189
  28. Swain, T., W. E. Hillis and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88.
  29. Voet, D. and J. G. Voet. 1990. Biochemistry, John Wiley & Sons, New York. 9, 1087-1095.
  30. Zhang, H. X., W. Wu, W. Chen, X. H. Gao, and L. S. Tang. 2005. Analysis of cordycepin and adenosine contents in fermentation supernatant of Cordyceps millitaris by HPLC. Acta Agriculture Shanghai 21, 53-56.

피인용 문헌

  1. Effect of silkworm varieties on Paecilomyces tenuipes culture vol.53, pp.2, 2015, https://doi.org/10.7852/jses.2015.53.2.87
  2. Biological Activity and Chemical Characteristics of Cordyceps militaris Powder Fermented by Several Microscopic Organisms vol.25, pp.2, 2015, https://doi.org/10.5352/JLS.2015.25.2.197
  3. Effects of LED on the growth of P. tenuipes vol.52, pp.1, 2014, https://doi.org/10.7852/jses.2014.52.1.59
  4. Antithrombotic Effect of Fermented Ophiopogon japonicus in Thrombosis-Induced Rat Models vol.20, pp.7, 2017, https://doi.org/10.1089/jmf.2016.3872