생명과학회지 (Journal of Life Science)

  • 제22권2호
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  • Pages.200-208
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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꽃송이버섯의 균사 생장을 위한 배지 조건 최적화

Optimization of Medium Composition for the Mycelial Growth of Sparassis crispa

  • 김진우 (경상북도산림환경연구원) ;
  • 천우재 (경상북도산림환경연구원) ;
  • 채경희 (경상북도산림환경연구원) ;
  • 김동관 ((주) 비트로시스 생명공학연구소) ;
  • 손성호 ((주) 비트로시스 생명공학연구소) ;
  • 김종국 (경북대학교 생명공학부) ;
  • 임희재 (경상북도산림환경연구원)
  • Kim, Jin-Woo (Gyeongsangbuk-Do Forest and Environment Research Institute) ;
  • Cheon, Woo-Jae (Gyeongsangbuk-Do Forest and Environment Research Institute) ;
  • Chai, Kyung-Hee (Gyeongsangbuk-Do Forest and Environment Research Institute) ;
  • Kim, Dong-Gwan (Institute of Biotechnology, Vitrosys Incoporation) ;
  • Son, Sung-Ho (Institute of Biotechnology, Vitrosys Incoporation) ;
  • Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Lim, Hee-Jae (Gyeongsangbuk-Do Forest and Environment Research Institute)
  • 투고 : 2011.12.05
  • 심사 : 2011.12.29
  • 발행 : 2012.02.28
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초록

본 연구에서는 균사의 액체 배양 특성 조사를 위하여 꽃송이버섯 균주 KGFS08과 KFRI746를 선발하였다. 균사 생장을 위한 우수 배지는 KTM 배지로 조사되었으며, 탄소원과 질소원은 각각 3% (w/v) starch와 0.3% (w/v) tryptone, 비타민은 folic acid로 나타났다. 따라서 액체 배양 최적 조건은 STK 배지[3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, 0.1% (w/v) folic acid], pH는 4.0~5.0으로 확인되었다.

The characteristics of mycelium growth of Sparassis crispa KGFS08 and KFRI746 in liquid culture were investigated. The optimum growth of the mycelium of S. crispa was observed in the KTM medium. The best carbon source was starch. In terms of nitrogen sources, tryptone affected mycelial growth in the liquid culture. The optimal culture conditions were pH 4.0-5.0 in STK medium [3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, and 0.1% (w/v) folic acid].

키워드

참고문헌

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피인용 문헌

  1. Cultivation Processes and Yield of Lentinula edodes on Surface Sawdust Bed vol.104, pp.3, 2015, https://doi.org/10.14578/jkfs.2015.104.3.434