생명과학회지 (Journal of Life Science)

  • 제22권2호
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  • Pages.177-183
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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감자유전자 StACRE의 분리 및 풋마름병 저항성 기능 검정

Isolation of Potato StACRE Gene and Its Function in Resistance against Bacterial Wilt Disease

  • 박상렬 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 차은미 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 김태훈 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 한세연 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 황덕주 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 안일평 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 조광수 (농촌진흥청 국립식량과학원 고령지농업연구센터) ;
  • 배신철 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과)
  • Park, Sang-Ryeol (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Cha, Eun-Mi (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Tae-Hun (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Se-Youn (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hwang, Duk-Ju (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Il-Pyung (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Kwang-Soo (Highland Agricultural Research Center (HARC), National Institute of Crop Science, Rural Development Administration) ;
  • Bae, Shin-Chul (Bio-Crop Development Division, National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2011.11.21
  • 심사 : 2011.12.26
  • 발행 : 2012.02.28
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초록

Ralstonia solanacearum (Rs)에 의해 유발되는 풋마름병은 감자 재배 시 발병하는 주요 병 중의 하나이다. 감자에서 풋마름병 저항성관련 유전자를 찾기 위해 기존에 기능이 알려진 다른 가지과 작물의 기능 유사 유전체를 이용하여 StACRE (HM749652) 유전자를 분리하고 염기서열을 분석하였다. 분리한 StACRE의 발현양상을 분석하기 위해 병 저항성 유도 신호전달 물질인 SA와 풋마름병원균 Rs (KACC10722)를 처리한 감자에서 RNA를 추출하여 RT-PCR을 실시한 결과 이 유전자는 SA 처리에 의해 3시간 후부터, Rs에 의해서는 12시간 후부터 발현이 현저하게 증가하였다. 따라서, 감자에서 이 유전자의 생물학적인 기능을 분석하기 위해 Gateway System을 이용하여 과발현용 vector를 만든 후 과발현 형질전환 감자를 제작하고 풋마름병균인 Rs를 접종하여 병 저항성 기능을 검정 한 결과 대조구인 수미 감자에 비해 병 저항성이 증대하였다.

Bacterial wilt (brown rot) caused by Ralstonia solanacearum (Rs) is one of the most devastating bacterial plant diseases in potatoes. To isolate bacterial wilt disease resistance-related genes from the potato, the StACRE (HM749652) gene was isolated and a sequenced search was performed using functional orthologs of Solanaceae from potatoes. StACRE is homologous to the tobacco NtACRE 132 protein and belongs to the ATL family involved in ubiquitination. To analyze the expression pattern of this gene, RT-PCR was performed with potato treated with salicylic acid (SA) and Rs (KACC 10722). StACRE was strongly induced 3 hours after treatment with SA and 12 hours after infection with Rs. To investigate its biological functions in the potato, we constructed a vector for overexpression in the potato by the Gateway system, and then generated transgenic potato plants. The gene expression of transgenic potato was analyzed by northern blot analysis. In the results of disease resistance assay in relation to bacterial wilt, StACRE overexpressed transgenic potato plants were shown to have more resistance than wild-type potato.

키워드

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