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Biophysical Feature, Crystallization and X-ray Crystallographic Studies of Toxascaris leonina Galectin

  • Sung, Min-Kyung (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Jeong, Mi-Suk (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Lee, Woo-Chul (Division of Magnetic Resonance, Korea Basic Science Institute) ;
  • Song, Jeong-Hyun (Division of Magnetic Resonance, Korea Basic Science Institute) ;
  • Kim, Hye-Yeon (Division of Magnetic Resonance, Korea Basic Science Institute) ;
  • Cho, Min-Kyoung (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Yu, Hak-Sun (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Jang, Se-Bok (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
  • 투고 : 2011.09.26
  • 심사 : 2011.11.21
  • 발행 : 2012.01.20

초록

Galectins are generally believed to be potential candidates for use in the development of novel antiinflammatory agents or as selective modulators of the immune response. In particular, galectin-9 exhibits some of the extracellular functions, including cell aggregation, adhesion, chemoattraction, activation, and apoptosis. Tl-galectin (Tl-gal, galectin-9 homologue gene) was isolated from an adult worm of the Toxascaris leonina. The full-length Tl-gal gene, which was incorporated into pET-28a, was overexpressed in E. coli and purified by nickel affinity and gel filtration chromatographies. The purified Tl-gal was crystallized using the hangingdrop vapor-diffusion method. The crystal belonged to the tetragonal space group $P4_1$, with unit-cell parameters of a = b = $75.7\AA$ and c = $248.4\AA$. The crystals were obtained at $20^{\circ}C$ and diffracted to a resolution of $3.0\AA$. The asymmetric unit contained four molecules of Tl-gal, which gave a crystal volume per protein mass (Vm) of $2.8\AA^3Da^{-1}$ and a solvent content of 54.1%.

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참고문헌

  1. Reddy, A.; Fried, B. Parasitol. Res. 2007, 100, 921-927. https://doi.org/10.1007/s00436-006-0416-4
  2. Kim, J. Y.; Cho, M. K.; Choi, S. H.; Lee, K. H.; Ahn, S. C.; Kim, D. H.; Yu, H. S. Mol. Biochem. Parasitol. 2010, 174, 53-61. https://doi.org/10.1016/j.molbiopara.2010.06.014
  3. Elliott, D. E.; Summers, R. W.; Weinstock, J. V. Curr. Opin. Gastroenterol. 2005, 21, 51-58.
  4. Bebb, J. R.; Scott, B. B. Aliment. Pharmacol. Ther. 2004, 20, 151- 159.
  5. Gomez-Escobar, N.; Lewis, E.; Maizels, R. M. Exp. Parasitol. 1998, 88, 200-209. https://doi.org/10.1006/expr.1998.4248
  6. Gregory, W. F.; Blaxter, M. L.; Maizels, R. M. Molecular & Biochemical Parasitology 1997, 87, 85-95. https://doi.org/10.1016/S0166-6851(97)00050-9
  7. Yenbutr, P.; Scott, A. L. Infect Immun. 1995, 63, 1745-1753.
  8. Joseph, G. T.; Huima, T.; Lustigman, S. Mol. Biochem. Parasitol. 1998, 96, 177-183. https://doi.org/10.1016/S0166-6851(98)00094-2
  9. Thomas, P. G.; Carter, M. R.; Atochina, O.; Da'Dara, A. A.; Piskorska, D.; McGuire, E.; Harn, D. A. Journal of Immunology 2003, 171, 5837-5841. https://doi.org/10.4049/jimmunol.171.11.5837
  10. Murray, J.; Gregory, W. F.; Gomez-Escobar, N.; Atmadja, A. K.; Maizels, R. M. Mol. Biochem. Parasitol. 2001, 118, 89-96. https://doi.org/10.1016/S0166-6851(01)00374-7
  11. Beall, M. J.; McGonigle, S.; Pearce, E. J. Mol. Biochem. Parasitol. 2000, 111, 131-142. https://doi.org/10.1016/S0166-6851(00)00307-8
  12. Rabinovich, G. A.; Baum, L. G.; Tinari, N. et al. Trends in Immunology 2002, 23, 313-320. https://doi.org/10.1016/S1471-4906(02)02232-9
  13. Rubinstein, N.; Ilarregui, J. M.; Toscano, M. A.; Rabinovich, G. A. Tissue Antigens. 2004, 64, 1-12. https://doi.org/10.1111/j.0001-2815.2004.00278.x
  14. Moore, K. W.; de Waal, M.; Coffman, R. L.; O'Garra, A. Annu. Rev. Immunol. 2001, 19, 683-765. https://doi.org/10.1146/annurev.immunol.19.1.683
  15. Gorelik, L.; Fields, P. E.; Flavell, R. A. J. Immunol. 2000, 165, 4773-4777. https://doi.org/10.4049/jimmunol.165.9.4773
  16. Chen, W.; Jin, W.; Hardegen, N.; Lei, K. J.; Li, L.; Marinos, N.; McGrady, G.; Wahl, S. M. J. Exp. Med. 2003, 198, 1875-1886. https://doi.org/10.1084/jem.20030152
  17. Gerald, B. CD spectroscopy deconvolution, version 2.1 1997.
  18. Leslie, A. G. W. Acta Cryst. 2006, D62, 48-57.
  19. Otwinowski, Z.; Minor, W. Methods Enzymol. 1997, 276, 307- 326. https://doi.org/10.1016/S0076-6879(97)76066-X
  20. Zilberstein, D.; Shapira, M. Annu. Rev. Microbiol. 1994, 48, 449- 470. https://doi.org/10.1146/annurev.mi.48.100194.002313
  21. Pan, A. A.; Pan, S. C. Exp. Parasitol. 1986, 62, 254-265. https://doi.org/10.1016/0014-4894(86)90030-5