생명과학회지 (Journal of Life Science)

  • 제22권1호
  • /
  • Pages.117-125
  • /
  • 2012
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

타크린으로 유발한 간세포 독성에 대한 효소별 굴 가수분해물의 보호 효과

Hepatoprotective Effects of Various Enzyme Hydrolysates from Oysters on Tacrine-Induced Toxicity in Human Hepatoma Cells

  • 박혜진 (신라대학교 대학원 생명공학과) ;
  • 도형주 (신라대학교 대학원 생명공학과) ;
  • 김옥주 (신라대학교 대학원 생명공학과) ;
  • 김안드레 (신라대학교 제약공학과) ;
  • 하종명 (신라대학교 대학원 생명공학과)
  • Park, Hye-Jin (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Do, Hyung-Joo (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Kim, Ok-Ju (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Kim, Andre (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Graduate school, Silla University)
  • 투고 : 2011.12.02
  • 심사 : 2012.01.19
  • 발행 : 2012.01.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 굴 가수분해물의 간 보호 효과를 확인하는 것을 목표로 하였다. 굴은 많은 기능적 요소를 가지고 있다고 알려져 있으며 특히, 효소에 의해 생산된 가수분해물은 우수한 기능적 물질을 포함한다. 타크린으로 유발한 간세포 독성에 대한 효소별 굴 가수분해물의 보호 효과를 확인하기 위하여 HepG2세포를 이용하여 in vitro상에서 확인하였다. 사용한 샘플은 Neutrase, Flavourzyme, Protamex을 이용하여 효소 가수분해한 것이다. 타크린으로 손상을 유발한 간세포에서는 GOT와 LDH가 증가하게 된다. 굴 효소 가수분해물을 처리한 실험군에서는 아무런 처리를 하지 않은 실험군에 비하여 높은 세포 생존율을 확인할 수 있었다. 또한 GOT와 LDH 역시 감소하는 것을 알 수 있었다. 본 연구를 통하여 타크린 독성에 대한 신약, 식품의 기초 자료를 제공할 수 있을 것으로 기대된다.

This study investigated the potential hepatoprotective benefits of Crassostrea gigas oyster hydrolysates. Oysters are known to have many biofunctional properties. In particular, oyster enzymatic hydrolysates produce substances with beneficial functions. The potential hepatoprotective effects of C. gigas hydrolysates against damage induced by tacrine were evaluated in vitro in HepG2 cells. Peptides were generated from C. gigas by enzymatic hydrolysis with Neutrase, Flavourzyme, or Protamex enzyme preparations. Tacrine treatment induced considerable cell damage in HepG2 cells, as shown by significant leakage of glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH). Cells treated with C. gigas hydrolysates showed an increased resistance to oxidative challenge compared to control cells, as revealed by higher cell survival against tacrine-induced hepatotoxicity. In addition, treatment with C. gigas hydrolysates reduced the leakage of GOT and LDH. These findings indicate that enzyme hydrolysates derived from C. gigas may be of benefit for developing hepatoprotective foods and drugs.

키워드

참고문헌

  1. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
  2. Bouilly, K., B. Gagnaire, M. Bonnard, G. H. Thomas, T. Renault, P. Miram, and S. Lspegue. 2006. Effects of cadmium on aneuploidy and hepocyte paramenters in the Pacific oyster, Crassostrea gigas. Aquat. Toxicol. 78, 149-156. https://doi.org/10.1016/j.aquatox.2006.02.028
  3. Brand-Williams, W., M. E. Cuvelier, and C. Berset. 1995. Use of free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaftund Technologie 28, 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  4. Cho, E. J. and S. H. Yoon. 1999. Protective effect of asiasari radix on rat liver. J. Korean Soc. Hygienic Sci. 5, 85-91.
  5. Chobert, J. M., C. Bertrand-Harb, and M. G. Nicolus. 1988. Solubility and emulsifying properties of caseins and whey proteins modified enzymatically by trypsin. J. Agric. Food Chem. 36, 883-892. https://doi.org/10.1021/jf00083a002
  6. Jang, Y. B. 2007. Application in foodstuffs and functional characteristics of oyster Hydrolysates. Ph. D. thesis, Pukyoung University, Pusan, Korea.
  7. Jeong, B. Y., B. D. Choi, and J. S. Lee. 1998. Protamate composition, cholesterol and α-tocopherol content in 72 species of Korean fish. J. Korean fish. Sci. Tech. 1, 129-146.
  8. Jeong, J. H., J. J. Wee, J. Y. Shin, J. H. Cho, and D. H. Jung. 2005. Antioxidative effect of crude saponin fraction prepared from culture product of basidiomycota cultured with fresh ginseng as substrate. J. Korean Food Sci. Technol.
  9. Jim, L. K. and J. P. Gee. Adverse effects of drugs on the liver. In Young, L. Y. and Koda-Kimble MA (ed.), Applied therapeutics: The clinical use of drugs, 26-1-26-17, Applied Therapeytics, Inc., Vancouver.
  10. Jun, S. Y., P. J. Park, and W. K. Jung. 2004. Purification and characterizaion of an antioxidative peptide from enzymatic hydrolysate of yellofin sole (Limand aspera) frame protein. Eur. Food Res. Technol. 219, 20-26. https://doi.org/10.1007/s00217-004-0882-9
  11. Kang, H. I., S. H. Kim, and J. H. Pyeun. 1974. Evaluation in the utility of the by-products of oyster processing. J. Korean Fish. Soc. 7, 37-40.
  12. Kang, K. H., S. Y. Kim, and D. K. Park. 1985. Special Topics in Food Chemistry. pp. 168-172, YUHANSA
  13. Kim, C. Y., J. H. Pyeun, and J. N. Nam. 1981. Decomposition of glycogen and protein in pickled oyster during fermemtation with salt. J. Korean Fish. Soc. 14, 66-71.
  14. Kim, S. K., Y. T. Kim, H. G. Byun, K. S. Nam, D. S. Joo, and F. Shahidi. 2001. Isolation and characterization of antioxidative peptidess from gelatin hydrolysate of Alaska pollack skin. J. Agric. Food Chem. 49, 1984-1989. https://doi.org/10.1021/jf000494j
  15. Kim, S. M. and J. U. Ha. 1995. Utilization of the protein hydrolysates of skipjack tuna viscera. J. Korean Food Sci. Technol. 27, 141-146.
  16. Kim, Y. K., Y. H. Kim, D. H. Kim, and K. T. Lee. 2005. Cytoprotective effects of natural flavonoids on carbon tetrachloride- induced toxicity in primary cultures of rat hepatocytes. J. Korean Pharmacogn. 36, 224-228.
  17. Kinsella, J. E. and K. J. Shetty. 1976. Functional properties of proteins in foods: A survey. Crit. Rev. Food Sci. Nutr. 7, 219.
  18. Knasmuller, S., W. Parzefall, R. Sanyal, S. Ecker, C. Schwab, M. Uhl, V. Mersch-Sundermann, G. Williamson, G. Hietsch, T. Langer, F. Darroudi, and A. T. Natarajan. 1998. Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens. Mutat. Res. 402, 185-202. https://doi.org/10.1016/S0027-5107(97)00297-2
  19. Lee, C. H. 1992. Application and development of protein sources. J. Korean Food Sci. Ind. 25, 93-100.
  20. Lee, E. G., K. B. Kim, and J. M. Jeong. 2006. Hepatoprotective effects of poly herbal formulation (Hepa-1000) on t-BHP-induced toxicity in human hepatoma cells. J. Korean Soc. Food Sci. Nutr. 35, 1121-1126. https://doi.org/10.3746/jkfn.2006.35.9.1121
  21. Lu, X., D. Akiyama, and K. Hirabayashi. 1994. Production of silk powder and properties. J. Seric. Jpn. 63, 21-27.
  22. McCord, J. M. and I. Fridovich. 1969. Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244, 6049-6055.
  23. Ministry of Maritime Affairs and Fisheries. 2004. pp. 146, Fishery Production Survey.
  24. National Fisheries Research and Development Agency. 1995. Supplemented Chemical Composition of Marine Products in Korea. pp. 139-143, Yemoon Publishing Co., Seoul.
  25. National Fisheries Research Development. 1995. Chemical Composition of Marine Products in Korea. pp. 146.
  26. Osseni, R. A., C. Debbasch, M. O. Christen, P. Rat, and J. M. Warnet. 1999. Tacrine-induced reactive oxygen species in a human liver cell line: the role of anethole dithilethione as a scavenger. Toxicol. In Vitro 13, 683-688. https://doi.org/10.1016/S0887-2333(99)00050-8
  27. Panyam, D. and A. Kilara. 1996. Enhancing the functionality of food proteins by enzymatic modlfication. Trends Food Sci. Technol. 7, 120-125. https://doi.org/10.1016/0924-2244(96)10012-1
  28. Ranathunga, S., N. Rajapakes, and S. K. Kim. 2006. Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster). Eur. Food Res. Technol. 222, 310-315. https://doi.org/10.1007/s00217-005-0079-x
  29. Rupnow, J. 1994. Proteins: biochemistry and application. Vol. 3, pp. 2182. In Encyclopedia of Food Science and Technology. In Hui, Y. H. (ed). John Willy and Son Inc. NY, USA.
  30. Samsumg hospital. http://www.samsunghospital.com/html/ boardimg/medi_webzine/pdf/10-07.pdf
  31. Sohn, D. H., Y. C. Kim, S. H. Oh, E. J. Park, X. Li, and B. H. Lee. 2003. Hepatoprotective and free radical scavenging effects of Nelumb nucifera. Phytomedicine 10, 165-169. https://doi.org/10.1078/094471103321659889
  32. Tanaka, K., I. Ikeda, A. Kase, K. Koda, S. Nishizono, T. Aoyama, and K. Imaizumi. 2003. Effects of feeding oyster, Crassostrea gigas, on serum and liver lipid levels in rat. J. Nutr. Sci. Vitaminol. 49, 100-106. https://doi.org/10.3177/jnsv.49.100
  33. Wang, J. and A. Wendel. 1990. Studies on the hepatotoxicity of galactosamine endotoxin or galatosamine/TNF in the perused mouse liver. Bioche. Pharmaco. 39, 267-270. https://doi.org/10.1016/0006-2952(90)90025-G
  34. Watkins, P. B., H. J. Zimmermann, M. J. Knapp, S. I. Gracon, and K. W. Lewis. 1994. Hepatotoxic effects of tacrine administration in patients with alzheimer's disease. J. A. Med. Assoc. 271, 992-998. https://doi.org/10.1001/jama.1994.03510370044030
  35. Wu, C. H., H. M. Chen, and C. Y. Shiau. 2003. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Eur. Food Res. Int. 36, 949-957. https://doi.org/10.1016/S0963-9969(03)00104-2
  36. Yoon, H. D., H. S. Byun, S. J. Chun, S. B. Kim, and Y. H. Park. 1986. Lipid composition of oyster, arkshell and sea-messel. J. Korean Fish. Soc. 19, 321-326.

피인용 문헌

  1. Optimization of Coho Salmon Hydrolysate Using Japanese Squid Liver and Its Properties vol.42, pp.11, 2013, https://doi.org/10.3746/jkfn.2013.42.11.1759