Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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A Phylogenetic Relationship between Foreign and Korean Strains of Flammulina velutipes Identified by rDNA-ITS Sequence Analysis

Flammulina velutipe의 국내 균주와 외래 균주 간의 ITS region을 이용한 계통학적 유연관계 분석

  • Hwang, Gwang-Rip (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Woo, Ju-Ri (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Co.) ;
  • Lee, Sang-Han (Food & Bio-Industry Research Institute and Department of Food Science & Technology) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
  • 황광립 (경북대학교 생명과학부) ;
  • 우주리 (경북대학교 생명과학부) ;
  • 윤혁준 (경북대학교 생명과학부) ;
  • 이창윤 (그린피스) ;
  • 이상한 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김종국 (경북대학교 생명과학부)
  • Received : 2011.11.03
  • Accepted : 2012.01.18
  • Published : 2012.01.30
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Abstract

This study was carried out to investigate the genetic relationship of Flammulina velutipes with other species. The ribosomal DNA cluster containing 4 rRNA genes from F. velutipes 4154 were sequenced. The length of the rDNA cluster sequence was estimated at 7,403 bp long and consisted of 1,806 bp of SSU rDNA, 245 bp of ITS 1 region, 159 bp of 5.8S rDNA, 308 bp of ITS 2 region, 3,402 bp of LSU rDNA, 1,400 bp of IGS 1 region, and 83 bp of 5S rDNA. The F. velutipes 4154 genes were contained in the rDNA cluster of F. velutipes in the order of SSU rDNA - ITS 1 - 5.8S rDNA - ITS 2 - LSU rDNA - IGS 1 - 5S rDNA. The phylogenetic relationships of 20 strains of Tricholomataceae and Physalacriaceae were analyzed by conducting distance analysis using the Neighbor-joining (NJ) method. The 20 strains used in this study were divided into three groups and the strains of the genus Flammulina were related very closely to strains of Physalacria bambusae.

본 연구는 팽이버섯(Flammulina velutipes)의 국내 균주와 외래 균주간의 유전적 유연관계를 조사하기 위하여 수행되었으며, 계통수 측정 결과 3개 그룹으로 나눠짐을 확인하였다. 20개 F. velutipes 균주들의 ITS region 염기 서열을 확보하였으며, 이들 서열을 바탕으로 multiple alignment를 보았으며, Neighbor-joining method를 이용하여 계통수를 작성하였으며, 2개 그룹으로 나눠짐을 확인하였다. 또한 F. velutipes 4154 균주의 rDNA-cluster를 최초로 분석한 결과, 총 10,974 bp임을 밝혔다. SSU는 1,806 bp, ITS region은 553 bp의 염기배열이 결정되었다. ITS 1 부분은 245 bp이고 ITS 2는 308 bp였으며, LSU에 해당되는 염기서열은 3,402 bp, IGS 1은 1,400 bp, 5S는 83 bp, IGS 2는 3,571 bp임을 확인하였다.

Keywords

References

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