Reproductive and Developmental Biology

  • 제36권4호
  • /
  • Pages.255-260
  • /
  • 2012
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Successful In Vitro Development of Preantral Follicles Isolated from Vitrified Mouse Whole Ovaries

  • Kim, Dong-Hoon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • No, Jin-Gu (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Jong-Ju (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Yoo, Jae Gyu (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 투고 : 2012.11.17
  • 심사 : 2012.12.14
  • 발행 : 2012.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of this study was to assess follicular viability and competence through in vitro culture of preantral follicles isolated from vitrified mouse whole ovaries. Mouse preantral follicles were enzymatically isolated from vitrified- warmed and fresh ovaries and cultured for 10 days followed by in vitro oocyte maturation. In vitro matured oocytes were fertilized and cultured to the blastocyst stage. Five minutes pre-exposure to vitrification solution of whole ovaries had significantly higher (p<0.05) oocyte survival and maturation rates than between 10 min exposure groups. Oocyte diameter was significantly smaller (p<0.05) in the 5 and 10 min exposure groups ($69.4{\pm}2.8$ and $67.8{\pm}3.1$) when compared to that of control group ($71.7{\pm}2.1$). There was no statistical significant difference in blastocyst development rates between vitrification group (8.6%) and the fresh control group (12.0%). The mean number of cells per blastocyst was significantly lower (p<0.05) in the vitrification group ($41.9{\pm}20.2$) than in the fresh control group ($55.1{\pm}22.5$). The results show that mouse oocytes within preantral follicles isolated from the vitrified whole ovaries can achieve full maturation, normal fertilization and embryo development.

키워드

참고문헌

  1. Aubard Y, Newton H, Scheffer G, Gosden RG (1998): Conservation of the follicular population in irradiated rats by the cryopreservation and orthotopic autografting of ovarian tissue. Eur J Obstet Gynecol Reprod Biol 79:83-87. https://doi.org/10.1016/S0301-2115(98)00044-X
  2. Bos-Mikich A, Marques L, Rodrigues JL, Lothhammer N, Frantz N (2012): The use of a metal container for vitrification of mouse ovaries, as a clinical grade model for human ovarian tissue cryopreservation, after different times and temperatures of transport. J Assist Reprod Genet, Oct 9. [Epub ahead of print].
  3. Candy CJ, Wood MJ, Whittingham DG (1997): Effect of cryoprotectants on the survival of follicles in frozen mouse ovaries. J Reprod Fertil 110:11-9. https://doi.org/10.1530/jrf.0.1100011
  4. Chen SU, Chien CL, Wu MY, Chen TH, Lai SM, Lin CW, Yang YS (2006): Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice. Hum Reprod 21:2794-2800. https://doi.org/10.1093/humrep/del210
  5. Cortvrindt R, Smitz J, Van Steirteghem AC (1996): A morphological and functional study of the effect of slow freezing followed by complete in vitro matura tion of primary mouse ovarian follicles. Hum Reprod 11:2648-2655. https://doi.org/10.1093/oxfordjournals.humrep.a019187
  6. Cox CL, Shaw J, Jenkin G (1996): Transplantation of cryopreserved ovarian tissue to adult recipients in mice. J Reprod Fetil 107:315-322. https://doi.org/10.1530/jrf.0.1070315
  7. Daniel JC Jr, Fox JM, Richardson P (1983): Return to partial function of rabbit ovaries after cryopreservation in liquid nitrogen. Theriogenology 19:405-412. https://doi.org/10.1016/0093-691X(83)90096-1
  8. dela Pena EC, Takahashi Y, Katagiri S, Atabay EC, Nagano M (2002): Birth of pups after transfer of mouse embryos derived from vitrified preantral follicles. Reproduction 123:593-600. https://doi.org/10.1530/rep.0.1230593
  9. Gook DA, Edgar DH, Stern C (1998): Effect of cooling rate and dehydration regimen on the histological appearance of human ovarian cortex following cryopreservation in 1,2-propanediol. Hum Repord 14:2061- 2068.
  10. Gosden RG, Baird DT, Wade JC, Webb R (1994): Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -$196^{\circ}C$. Hum Reprod 9:597-603.
  11. Gunasena KT, Villines PM, Critser ES, Critser JK (1997): Live births after autologous transplant of cryopreserved mouse ovaries. Hum Repord 12:101-106. https://doi.org/10.1093/humrep/12.1.101
  12. Hasegawa A, Hamada Y, Mehandjiev T, Koyama K (2004): In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries. Fertil Steril 81(Suppl.):824-830. https://doi.org/10.1016/j.fertnstert.2003.08.028
  13. Hasegawa A, Mochida N, Ogasawara T, Koyama K (2006): Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization. Fertil Steril. 286(4 Suppl):1182-1192.
  14. Kim DH, Chi HJ, Kang HG, Han SW, Lee HT, Chung KS, Lee HJ (1999): Effect of gonadotrophins on in vitro growth and maturation of mouse preantral follicles. Korean J Anim Reprod 23(1):53-61
  15. Kim GA, Kim HY, Kim JW, Lee G, Lee E, Ahn JY, Park JH, Lim JM (2011): Effectiveness of slow freezing and vitrification for long-term preservation of mouse ovarian tissue. Theriogenology. 75(6):1045-1051. https://doi.org/10.1016/j.theriogenology.2010.11.012
  16. Liu J, Van der Elst J, Ven den Broeke, Dhont M (2001): Live offspring by in vitro fertilization of oocytes from cryopreserved primordial mouse follicles after sequential in vivo transplantation and in vitro maturation. Biol Reprod 64:171-178. https://doi.org/10.1095/biolreprod64.1.171
  17. Migishima F, Suzuki-Migishima R, Song S-Y, Kuramochi T, Azuma S, Nishijima M, Yokoyama M (2003): Successful cryopreservation of mouse ovaries by vitrification. Biol Reprod 68:881-887. https://doi.org/10.1095/biolreprod.102.007948
  18. Newton H, Illingworth P (2001): In-vitro growth of murine pre-antral follicles after isolation from cryopreserved ovarian tissue. Hum Repord 16:423-429. https://doi.org/10.1093/humrep/16.3.423
  19. Oktay K, Newton H, Aubard Y, Salha O, Gosden RG (1998): Cryopreservation of immature human oocytes and ovarian tissue - an emerging technology? Fertil Steril 69:1-7. https://doi.org/10.1016/S0015-0282(97)00207-0
  20. Oktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden RG (1997): Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue. Fertil Steril 67:481-6. https://doi.org/10.1016/S0015-0282(97)80073-8
  21. Parrott DMV (1960): The fertility of mice with orthotopic ovarian grafts derived from frozen tissue. J Reprod Fertil 1:230-241. https://doi.org/10.1530/jrf.0.0010230
  22. Rall WF, Fahy GM (1985): Ice-free cryopreservation of mouse embryos at -$196^{\circ}C$ by vitrification. Nature 313:573-575. https://doi.org/10.1038/313573a0
  23. Sugimoto M, Maeda S, Manabe N, Miyamoto H (2000): Development of infantile rat ovaries autotransplanted after cryopreservation by vitrification. Theriogenology 53:1093-1103. https://doi.org/10.1016/S0093-691X(00)00255-7
  24. Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H (1998): Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Repord Dev 51:53-58. https://doi.org/10.1002/(SICI)1098-2795(199809)51:1<53::AID-MRD6>3.0.CO;2-V
  25. Wang X, Catt S, Pangestu M, Temple-Smith P (2011): Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births. Reproduction 141 (2):183-191. https://doi.org/10.1530/REP-10-0383