Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지

Identification and Characterization of LHX8 DNA Binding Elements

  • Park, Miree (Dept. of Biomedical Science, CHA University) ;
  • Jeon, Sanghyun (Dept. of Biomedical Science, CHA University) ;
  • Jeong, Ji-Hye (Dept. of Biomedical Science, CHA University) ;
  • Park, Miseon (Fertility Center of CHA Gangnam Medical Center, CHA University) ;
  • Lee, Dong-Ryul (Dept. of Biomedical Science, CHA University) ;
  • Yoon, Tae Ki (Fertility Center of CHA Gangnam Medical Center, CHA University) ;
  • Choi, Dong Hee (Dept. of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University) ;
  • Choi, Youngsok (Dept. of Biomedical Science, CHA University)
  • Received : 2012.10.04
  • Accepted : 2012.11.17
  • Published : 2012.12.31
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Abstract

Lhx8 (LIM homeobox 8) gene encodes a LIM homeodomain transcriptional regulator that is preferentially expressed in germ cells and critical for mammalian folliculogenesis. However, Lhx8 DNA binding sequences are not characterized yet. We aimed to identify and characterize a cis-acting sequence of germ-cell specific transcriptional factor, Lhx8. To identify Lhx8 DNA binding element, Cyclic Amplification of Sequence Target (CAST) Analysis was performed. Electrophoretic Mobility Shift Assay (EMSA) was processed for the binding specificity of Lhx8. Luciferase assay was for the transcriptional activity of Lhx8 through identified DNA binding site. We identified a putative cis-acting sequence, TGATTG as Lhx8 DNA binding element (LBE). In addition, Lhx8 binds to the LBE with high affinity and augments transcriptional activity of luciferase reporter driven by artificial promoter containing the Lhx8 binding element. These findings indicate that Lhx8 directly regulates the transcription of genes containing Lhx8 binding element in oocytes during early folliculogenesis.

Keywords

References

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