한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제16권4호
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  • Pages.329-338
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  • 2012
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

Decreased Contact Inhibition in Mouse Adipose Mesenchymal Stem Cells

  • Jeon, Yunmi (Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Sungshin Womens University) ;
  • Lee, Myung Sook (Dept. of Food and Nutrition, Sungshin Womens University) ;
  • Cheon, Yong-Pil (Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Sungshin Womens University)
  • 투고 : 2012.11.14
  • 심사 : 2012.12.09
  • 발행 : 2012.12.31
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초록

The proliferation of embryonic cells or adult stem cells in tissue is critically regulated during development and repair. How limited the proliferation of cells, so far, is not much explored. Cell-cell contact proliferation inhibition is known as a crucial mechanism regulating cell proliferation in vitro and in vivo. In this study we examined the characters of mouse subcutaneous adipose derived stem cells (msADSC) whether they lost or get contact inhibition during in vitro culture. The characters of msADSC growth after confluence were analyzed using confocal microscope and the expression profiles of contact inhibition related genes were analyzed according to the morphological changes using real-time PCR method. msADSC showed overlapping growth between them but not after passage 14. The cell shapes were also changed after passage 14. The expression profiles of genes which are involved in contact inhibition were modified in the msADSC after passage 14. The differentiation ability of msADSCs to adipocyte, chondrocyte and osteocyte was not changed by such changes of gene expression profiles. Based on these results, it is revealed that smADSC were characterized by getting of strong cell-cell contact inhibition after passage 14 but the proliferation and developmental ability were not blocked by the change of cell-cell contact proliferation inhibition. These finding will help to understand the growth of adipose tissue, although further studies are needed to evaluate the physiological meaning of the cell-cell contact proliferation inhibition during in vitro culture of msADSC.

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참고문헌

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