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Gallotannin regulates apoptosis and COX-2 expression via Akt and p38kinase pathway in human lung cancer cell line, A549

  • Yu, Seon-Mi (Department of Biological Sciences, College of Natural Sciences, Kongju National University) ;
  • Gweon, Eun-Jeong (Department of Biological Sciences, College of Natural Sciences, Kongju National University) ;
  • Chung, Ki-Wha (Department of Biological Sciences, College of Natural Sciences, Kongju National University) ;
  • Kim, Kwang-Hoon (Department of Biological Sciences, College of Natural Sciences, Kongju National University) ;
  • Cho, Hong-Sik (University of Tennessee Health Science Center) ;
  • Kim, Song-Ja (Department of Biological Sciences, College of Natural Sciences, Kongju National University)
  • 투고 : 2011.10.24
  • 심사 : 2012.05.21
  • 발행 : 2012.10.31

초록

Gallotannin (GT) is derived from plant poly phenol and is associated with biological actions in a wide range of cells. In this study, we evaluated the effect of GTon apoptosis and cyclooxygenase-2 (COX-2) expression and attempted to shed light on the mechanism of action in A549 human lung carcinoma cells. We found that GT dramatically induced apoptosis as demonstrated by expression of p53 and active caspase-3 via western blot analysis and fragmented DNA as detected by DNA fragmentation and DAPI staining. We also observed that GT significantly causes COX-2 expression in a dose-dependent manner determined by western blot analysis. Phosphorylation of Akt and p38 was considerably increased by GT in A549 human lung carcinoma cells. Inhibition of Akt and p38kinase with LY294002 or SB203580 suppressed GT-induced apoptosis and COX-2 expression. Furthermore, we have shown that prevention of COX-2 with NS398 or indomethacin does not any effects on apoptosis induced by GT. Taken together, our present results suggest that GT regulates apoptosis and COX-2 expression through Akt and p38kinase pathway in A549, human lung carcinoma cells.

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피인용 문헌

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  2. Suppression of E-cadherin Mediates Gallotannin Induced Apoptosis in Hep G2 Hepatocelluar Carcinoma Cells vol.10, pp.5, 2012, https://doi.org/10.7150/ijbs.7495
  3. Isoform-Specific Role of Akt in Oral Squamous Cell Carcinoma vol.9, pp.7, 2012, https://doi.org/10.3390/biom9070253
  4. Inhibitory Effect of Gallotannin on Lung Metastasis of Metastatic Colorectal Cancer Cells by Inducing Apoptosis, Cell Cycle Arrest and Autophagy vol.49, pp.6, 2012, https://doi.org/10.1142/s0192415x21500725