한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제25권6호
  • /
  • Pages.745-752
  • /
  • 2012
  • /
  • 1226-3591(pISSN)
  • /
  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

Biomass and Molecular Characteristics of Multi-tillering Miscanthus Mutants

  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University) ;
  • Zhang, Lili (Department of Horticultural Science, Mokpo National University) ;
  • Choi, Young In (Department of Horticulture, Chungnam National University) ;
  • Chung, Sung Jin (Department of Horticulture, Chungnam National University) ;
  • Yoo, Yong Kweon (Department of Horticultural Science, Mokpo National University) ;
  • Kim, Dong Sub (Radiation Breeding and Genetics, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Radiation Breeding and Genetics, Korea Atomic Energy Research Institute)
  • 투고 : 2012.11.02
  • 심사 : 2012.12.03
  • 발행 : 2012.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Compared to wide ranges of genetic variation of natural populations, very limited Miscanthus cultivar has been released. This study was the first report on the development of Miscanthus cultivar by means of radiation breeding. Seeds of M. sinensis were initially exposed to gamma rays of 250 Gy for 24 h, generated from a $^{60}Co$ gamma-irradiator. The irradiated seeds were sown and then the highly tiller-producing mutants were selected for this study. Biomass-related parameters including tiller number, plant height, stem diameter, and leaf number were measured. Ploidy level and internal transcribed spacer (ITS) were investigated to characterize the mutants compared to wild type (WT) Miscanthus. Plant height and tiller number were negatively related, where multi-tillering mutants were relatively short after 4 month growth. However stem diameter and leaf number were greater in mutants. All the materials used in this study were diploid, implying that the mutants with greater tiller numbers and stem diameter were not likely related to polyploidization. Based on the sequence of ITS regions, the mutants demonstrated base changes from the gamma irradiation where G+C content (%) was decreased in the ITS1, but increased in ITS2 when compared to WT sequence. ITS2 region was more variable than in ITS1 in the mutants, which collectively allows identification of the mutants from WT. Those mutants having enhanced tillers and allelic variations might be used as breeding materials for enhanced biomass-producing Miscanthus cultivars.

키워드

참고문헌

  1. Beale, C.V. and S.P. Long. 1997. Seasonal dynamics of nutrient accumulation and partitioning in the perennial $C_{4}$-grasses Miscanthus ${\times}$ giganteus and Spartina cynosuroides. Biomass Bioenergy 12:419-428. https://doi.org/10.1016/S0961-9534(97)00016-0
  2. Brosse, N., A. Dufour, X. Meng, Q. Sun and A. Ragauskas. 2012. Miscanthus: a fast-growing crop for biofuels and chemicals production. Biofuel. Bioprod. Bior. 6:580-598. https://doi.org/10.1002/bbb.1353
  3. Chen, S., H. Yao, J. Han, C. Liu, J. Song, L. Shi, Y. Zhu, X. Ma, T. Gao, X. Pang, K. Luo, Y. Li, X. Li, X. Jia, Y. Lin and C. Leon. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 5(1):e8613. https://doi.org/10.1371/journal.pone.0008613
  4. Dhanavel, D., S. Gnanamurthy and M. Girija. 2012. Effect of gamma rays on induced chromosomal variation in cowpea Vigna unguiculata (L.) Walp. Int. J. Curr. Sci. 245-250.
  5. Eynon, R.T. 2011. Renewable energy consumption and electricity preliminary statistics 2010. http://www.eia.gov/ftproot/ renewables/pretrends10.pdf.
  6. Gong, L., Y.J. Yang, and J. Zhou. 2012. Genes involved in the synthesis and signaling pathway of strigolactone, a shoot branching inhibitor. Biol. Plantarum 56:210-214 https://doi.org/10.1007/s10535-012-0078-2
  7. Greef, J.M. and M. Deuter. 1993. Syntaxonomy of Miscanthus ${\times}$ giganteus GREEF et DEU. Angew. Bot. 67:87-90.
  8. Heaton, E.A., J. Clifton-Brown, T.B. Voigt, M.B. Jones and S.P. Long. 2003. Miscanthus for renewable energy generation: European Union experience and projections for Illinois. Mitigation and Adaptation Strategies for Global Change 9:433-451.
  9. Henry, R.J. 2010. Evaluation of plant biomass resources available for replacement of fossil oil. Plant Biotechnol. J. 8:288-293. https://doi.org/10.1111/j.1467-7652.2009.00482.x
  10. Hodkinson, T.R., M.W. Chase, S.A. Renvoize. 2002. Characterization of a genetic resource collection for Miscanthus (Saccharinae, Andropogonea, Poaceae) using AFLP and ISSR PCR. Ann. Bot. 89:627-636. https://doi.org/10.1093/aob/mcf091
  11. Jones, M.B. and M. Walsh. 2001. Miscanthus: For Energy and Fibre, James & James Ltd., London.
  12. Kim, O.T., K.H. Bang, D.S. In, J.W. Lee, Y.C. Kim, Y.S. Shin, D.Y. Hyun, S.S. Lee, S.W. Cha and N.S. Seong. 2007. Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences. Plant Biotechnol. Rep. 1:163-167. https://doi.org/10.1007/s11816-007-0019-2
  13. Kress, W.J., K.J. Wurdack, E.A. Zimmer, L.A. Weigt and D.H. Janzen. 2005. Use of DNA barcodes to identify flowering plants. P. Natl. Acad. Sci.(PNAS) 102:8369-8374. https://doi.org/10.1073/pnas.0503123102
  14. Lewandowski, I., J.C. Clifton-Brown, J.M.O. Surlock and S. Huisman. 2000. Miscanthus: European experience with a novel energy crop. Biomass Bioenergy 19:209-227. https://doi.org/10.1016/S0961-9534(00)00032-5
  15. Li, X., M. Lassner and Y. Zhang. 2002. Deleteagene: a fast neutron deletion mutagenesis-based gene knockout system for plants. Comp. Funct. Genom. 3:158-160. https://doi.org/10.1002/cfg.148
  16. McSteen, P. 2009. Hormonal regulation of branching in grasses. Plant Physiol. 149:46-55. https://doi.org/10.1104/pp.108.129056
  17. Pyter, R.J., F.G. Dohleman and T.B. Voigt. 2010. Effects of rhizome size, depth of planting and cold storage on Miscanthus ${\times}$ giganteus establishment in the Midwestern USA. Biomass Bioenergy 34:1466-1470. https://doi.org/10.1016/j.biombioe.2010.04.014
  18. Rayburn, A.L., J. Crawford, C.M. Rayburn and J.A. Juvik. 2009. Genome size of three Miscanthus species. Plant Mol. Biol. Rep. 27:184-188. https://doi.org/10.1007/s11105-008-0070-3
  19. Shadia, A.R., R.Y. Othman and M. Chai. 2002. Plojdy analysis and DNA content of mutant banana "Pisang Berangan" using flow cytometry. Biotropia 19:47-56.
  20. Sorensen, A., P.J. Teller, T. Hilstrom and B.K. Ahring. 2008. Hydrolysis of Miscanthus for bioenthanol production using dilute acid presoaking combined with wet explosion pretreatment and enzymatic treatment. Bioresource Technol. 99:6602-6607. https://doi.org/10.1016/j.biortech.2007.09.091
  21. Swaminathan, K., W.B. Chae, T. Mitros, K. Varala, L. Xie, A. Barling, K. Glowacka, M. Hall, S. Jezowski, R. Ming, M. Hudson, J.A. Juvik, D.S. Rokhsar and S.P. Moose. 2012. A framework genetic map for Miscanthus sinensis from RNAseqbased markers shows recent tetraploidy. BMC Genomics 13:142. https://doi.org/10.1186/1471-2164-13-142
  22. Tindall, K.R., J. Stein and F. Hutchinson. 1988. Changes in DNA base sequence induced by gamma-ray mutagenesis of lambda phage and prophage. Genetics 118:551-560.
  23. van Harten, A.M. 1998. Mutation Breeding: Theory and Practical Applications, Cambridge University Press, London.
  24. Venendaal, R., U. Jorgensen and C. Foster. 1997. European energy crops: a synthesis. Biomass Bioenergy 13:147-185. https://doi.org/10.1016/S0961-9534(97)00029-9
  25. Xia, K., R. Wang, X. Ou, Z. Fang, C. Tian, J. Duan, Y. Wang and M. Zhang. 2012. OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice. PLoS One 7(1):e30039. https://doi.org/10.1371/journal.pone.0030039
  26. Yasuno, N., Y. Yasui, I. Takamure and K. Kato. 2007. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice. J. Hered. 98:169-172. https://doi.org/10.1093/jhered/esl069

피인용 문헌

  1. In vitro physical mutagenesis of giant reed (Arundo donax L.) vol.9, pp.8, 2017, https://doi.org/10.1111/gcbb.12458