Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity and Anticancer Effects of Rough Rice (Oryza sativa L.) by Germination Periods

발아 기간에 따른 벼(Oryza sativa L.)의 항산화활성과 in vitro 항암활성

  • Kim, Hyun-Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Sang-Hoon (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Agrofood Resources, NAAS) ;
  • Kim, Tae-Myoung (College of Veterinary Medicine, Chungbuk National University) ;
  • Park, Dong-Sik (Dept. of Agrofood Resources, NAAS) ;
  • Kim, Jae-Hyun (Dept. of Agrofood Resources, NAAS) ;
  • Kim, Dae-Joong (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • Received : 2011.10.14
  • Accepted : 2011.11.08
  • Published : 2012.01.31
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Abstract

This study was conducted on the antioxidant activity and in vitro anticancer effects of rough rice (Oryza sativa L.) by germination periods. Rough rice was germinated at $37^{\circ}C$ for 8 days and then extracted with 70% ethanol. It was then analyzed for total polyphenol content, reducing power, antioxidant activities, and in vitro anticancer effects. Total polyphenol content increased from 3.12 mg/g for raw rough rice to 4.05 mg/g at 4 days of germination. Also reducing power increased from 0.96 to 1.25 (p<0.05). DPPH radical scavenging activity increased from 29.25% at 0 day to 34.82% at 2 days. ABTS radical scavenging activity increased from 3.05 mg AA eq/100 g at 0 day to 3.84 mg AA eq/100 g at 4 days, and then decreased afterward. The anticancer effect of ethanol extract at 4 days on stomach cancer cell line (AGS) and colon cancer cell line (HCT-116) showed higher values compared with raw rough rice extract. These results suggest that the best germination periods for increasing biological activities may be 3~4 days.

발아기간에 따른 발아 벼 추출물의 항산화성분, 항산화활성 및 암세포주 성장억제효과를 살펴보았다. 발아는 0, 2, 4, 6 및 8일 동안 진행하였으며, 발아 후 70% 에탄올로 추출물을 제조하였다. 발아가 진행됨에 따라 항산화성분 및 항산화활성은 2~4일까지는 증가하였다가 그 이후에는 감소하였다. 총 폴리페놀 함량은 발아 4일차에서 4.05 mg/g으로 가장 높았으며, DPPH 라디칼 소거능은 0, 2, 4, 6 및 8일차 각각 29.25, 34.82, 31.17, 26.27 및 18.51%로 발아 2일차에 가장 높았으며, 총 항산화력은 발아기간별로 각각 3.05, 3.17, 3.84, 2.43 및 2.167 mg AA eq/100 g으로 발아 4일차에서 가장 높게 나타났다. 환원력 또한 발아 4일차에 1.25로 가장 높게 나타났다. 암세포주 성장억제효과는 위암세포주(AGS) 보다 대장암세포주(HCT-116)에서 높게 나타났다. 이상의 결과로부터 생리활성 증가를 위해서 벼를 발아시킬 경우3~4일이 적당한 것으로 판단되며, 추후 새로운 물질의 생성과 생리활성 성분들에 대한 연구가 더 진행되어야 할 것으로 판단된다.

Keywords

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