Journal of The Korean Society of Agricultural Engineers (한국농공학회논문집)

The Korean Society of Agricultural Engineers (한국농공학회)
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Assessment of Instrument Efficiency in Detecting Airborne Virus

공기 중 바이러스 포집 장비의 효율성 평가

  • 하태환 (서울대학교 농업생명과학대학 지역시스템공학과 & 농업생명과학 연구원) ;
  • 이인복 (서울대학교 농업생명과학대학 지역시스템공학과 & 농업생명과학 연구원) ;
  • 권경석 (서울대학교 농업생명과학대학 지역시스템공학과 & 농업생명과학 연구원) ;
  • 이성복 (서울대학교 농업생명과학대학 지역시스템공학과 & 농업생명과학 연구원) ;
  • 송상현 (서울대학교 농업생명과학대학 지역시스템공학과 & 농업생명과학 연구원) ;
  • ;
  • 윤순식 (농림수산검역검사본부 역학조사과)
  • Received : 2011.12.09
  • Accepted : 2011.12.27
  • Published : 2012.01.31
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Abstract

In livestock industry, damage caused by the epidemic diseases such as Foot-and-Mouth Disease (FMD), Highly-Pathogenic-Avian-Influenza (HPAI) and Porcine-Reproductive-and-Respiratory-Syndrome (PRRS) was very serious. The financial loss incurred from FMD alone which occurred on Nov. 2011 in Korea was estimated at 3 billion won, 23 % of annual livestock industry production. The livestock industry in Korea has greater risk of disease infection because of high density production, etc. Investigating the spread of livestock diseases should consider both direct and indirect contact as well as other various factors including airborne. Airborne infection of livestock disease was first hypothesised in the early 1900s, however, field experimental studies are still limited. Furthermore, no protocol is available in detecting airborne viruses in the field. In this study, effective virus samplers were investigated by comparative analysis of the type of samplers used detect to airborne virus. Laboratory experiments were conducted to compare virus samplers such as Bio-sampler, Dust-sampler, Compact-Cascade-Impactor (CCI) and Microflow in detecting PRRSV. Samples were analyzed by Reverse-Transcription PCR to assess the efficiency of the instrument in detecting the airborne virus. First, samples were classified into five levels according to light intensity of gel images and then the classified results were normalized. In every case, Bio-sampler and Dust-sampler were comparable with each other and have shown to be more effective than CCI and Microflow samplers.

Keywords

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