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Biological Characterization of a Vibrio alginolyticus-Specific Bacteriophage

Vibrio alginolyticus에 대한 특이 bacteriophage의 생물학적 특성

  • Heo, Yong Ju (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Lee, Chan Heun (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Baek, Min Suk (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Ahn, Hyun Mi (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Hwang, Yo Sep (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Choi, Sanghoon (Department of Food Science and Biotechnology, Kunsan National University)
  • 허용주 (군산대학교 수산생명의학과) ;
  • 이찬흔 (군산대학교 수산생명의학과) ;
  • 백민석 (군산대학교 수산생명의학과) ;
  • 안현미 (군산대학교 수산생명의학과) ;
  • 황요셉 (군산대학교 수산생명의학과) ;
  • 박권삼 (군산대학교 식품생명공학과) ;
  • 최상훈 (군산대학교 식품생명공학과)
  • Received : 2012.08.03
  • Accepted : 2012.11.27
  • Published : 2012.12.31

Abstract

Vibrio alginolyticus, a marine fish and shellfish pathogen, has been found at a high frequency around the coastal areas of Korea. Vibrio alginolyticus was purified from various diseased fish, and a V. alginolyticus-specific bacteriophage was isolated from seawater obtained from fish farms located on the west coast of Korea. In a bacterial lysis experiment using the phage and antibiotics, tetracycline, $10^3$ cfu/ml of V. alginolyticus were completely lysed by both the phage and the antibiotic, suggesting that the purified phage in the study could be utilized as an alternative to antibiotics in the control of fish and shellfish diseases caused by V. alginolyticus.

Keywords

References

  1. Bruynoghe R and Maisin J. 1921. Essais de thérapeutique au moyen du bacteriophage. C R Soc Biol 85, 1120-1121.
  2. Chen D Hanna PU, Altmann K, Smith A, Moon P and Hammond LS. 1992. Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish, and shellfish. Appl Environ Microbiol 58, 3694-3700.
  3. Izumi S and Aranishi F. 2004. Relationship between gyrA mutations and quinolone resistance in Flavobacterium psychrophilum isolates. Appl Environ Microbiol 70, 3968-3972. https://doi.org/10.1128/AEM.70.7.3968-3972.2004
  4. Jayaprakash NS, Pai SS, Philip R and Singh IS. 2006. Isolation of a pathogenic strain of Vibrio alginolyticus from necrotic larvae of Macrobrachium rosenbergii (de Man). J Fish Dis 29, 187-191. https://doi.org/10.1111/j.1365-2761.2006.00690.x
  5. Ju Jw, Rhee GH and Kim I. 1987. Studies on the Phage of V. parahaemylticus. The J Kor Soc Microbiol 22, 61-70.
  6. Hwang KW, Oh BY, Gong YW, Lee JM, Go JM, Kim YH. 2007. Distribution and Antibiotic Resistance of Vibrio vulnif icus isolated in Incheon coastal area. J environ Sanit Engineer 22, 55-63.
  7. Lafrentz BR, LaPatra, SE, Call DR and Cain KD. 2008. Isolation of rifampicin resistant Flavobacterium psychrophilum strains and their potential as live attenuated vaccine candidates. Vaccine 26, 5582-5589. https://doi.org/10.1016/j.vaccine.2008.07.083
  8. Lee KK, Liu PC, Huang CY. 2003. Vibrio parahaemolyticus infections for both humans and edible mllusk abalone. Microbes Infect 5, 481-485. https://doi.org/10.1016/S1286-4579(03)00065-0
  9. Liu PC, Chen YC, Huang CY and Lee KK. 2003. Virulence of Vibrio parahaemolyticus isolated from cultured small abalone, Haliotis diversicolor supertexta, with withering syndrome. Lett Appl Microbiol 31, 433-437.
  10. Liu PC, Chen YC and Lee KK. 2001. Pathogenicity of Vibrio alginolyticus isolated from diseased small abalone Haliotis diversicolor supertexta. Microbios 104, 71-77.
  11. Oh BY, Kim JH, Gong YW, Jegal S, Kim HY, Lee MY, Hwang KW, Koh YJ, Lee JM, Go JM and Kim YH. 2007. Characteristics of Vibrio vulnif icus isolated Incheon. The Kor J Microbiol 43, 256-263.
  12. Park, MY, Kim HJ, Choi ST, Oh EG and Chang DS. 2002. Pathogenic Factors of Vibrio spp. Isolated from Seawater of Gwangan Beach in Busan. J Fish Sci, 5, 178-182. https://doi.org/10.5657/fas.2002.5.3.178
  13. Schmidt U, Chmel H and Cobbs C. 1979. Vibrio alginolyticus infections in humans. J Clin Microbiol 10, 666-668
  14. Smith S and Krueger AP. 1954. Characteristics of a new Vibrio bacteriophage system. J Gen Physiol 38, 161-168. https://doi.org/10.1085/jgp.38.2.161
  15. Spencer R. 1955. A marine bacteriophage. Nature 175, 690-691. https://doi.org/10.1038/175690a0
  16. Stenholm AR, Dalsgaard I and Middelboe M 2008. Isolation and characterization of bacteriophage infecting the fish pathogen Flavobacterium psychrophilum. Appl Environ Microbiol 74, 4070-4078. https://doi.org/10.1128/AEM.00428-08
  17. Twort FW. 1915. An investigation on the nature of ultra microscopic viruses. Lancet 2, 1241-1243.
  18. Yan Q, Chen Q, Ma S, Zhuang Z and Wang X. 2007. Characteristics of adherence of pathogenic Vibrio alginolyticus to the intestinal mucus of large yellow croaker (Pseudosciaena crocea). Aquaculture 269, 21-30. https://doi.org/10.1016/j.aquaculture.2007.02.042