Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Inhibition of Pathogenic Bacteria by Pediococcus pentosaceus Strain SH-10 Isolated from Hard Clam Meretrix meretrix Sikhae

백합(Meretrix meretrix) 식해에서 분리한 Pediococcus pentosaceus SH-10에 의한 병원성 세균의 억제 기작

  • Shin, Dong-Min (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Kim, Hee-Dai (Department of Biotechnology and Biomedicine, Chungbuk Provincial College) ;
  • Koo, Jae-Geun (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
  • 신동민 (군산대학교 식품생명공학과) ;
  • 김희대 (충북도립대학 바이오생명의약과) ;
  • 구재근 (군산대학교 식품생명공학과) ;
  • 박권삼 (군산대학교 식품생명공학과)
  • Received : 2012.10.08
  • Accepted : 2012.11.21
  • Published : 2012.12.31
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Abstract

In this study, we investigated the mechanism of inhibition of pathogenic bacteria by Pediococcus pentosaceus strain SH-10 isolated from hard Clam Meretrix meretrix sikhae. When P. pentosaceus SH-10 was co-cultured in MRS broth with pathogenic bacteria, including Bacillus cereus, Listeria monocytogenes, Salmonella choleraesuis and Staphyloccus aureus, no viable pathogenic cells were detected after 18 h of incubation. However, pediocin or a pediocin-like bacteriocin was not detected in cultures of P. pentosaceus SH-10 by the agar diffusion method. Organic acids were produced in MRS broth in proportion to the incubation time of P. pentosaceus SH-10. These results indicate that P. pentosaceus SH-10 inhibited the growth of pathogenic bacteria by lowering the pH of the growth medium through the production of organic acids, including sodium lactate, sodium acetate, and sodium citrate.

Keywords

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