Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Characterization of Growth and Auxin Formation in a Bacteria Isolated from Waste bed of Agaricus bisporus

양송이 수확 후 배지로 부터 분리한 옥신생산 세균의 생육특성

  • Shin, Sang-Kwon (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Kyung, Ki-Cheon (Taean Lily Experiment Station, Chungcheongnam-do Agricultural Research and Extention Services) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chung, Doug-Young (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • 신상권 (충남대학교 생물환경화학과) ;
  • 경기천 (충남농업기술원 태안백합시험장) ;
  • 공원식 (국립원예특작과학원 버섯과) ;
  • 정덕영 (충남대학교 생물환경화학과) ;
  • 윤민호 (충남대학교 생물환경화학과)
  • Received : 2012.10.15
  • Accepted : 2012.11.27
  • Published : 2012.12.31
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Abstract

An auxin-producing bacteria (2SJ8-02) was isolated from waste mushroom bed of Agaricus bisporus in Buyeo-Gun, Chungnam. The strain 2SJ8-02 was classified as a novel strain of Pantoea rwandensis based on a chemotaxanomic and phylogeneticanalyses. The isolate was confirmed to produce indole-3-acetic acid (IAA), one of auxin hormones, by TLC and HPLC analyses. The maximum concentration of IAA, $122mg\;L^{-1}$ was detected from the culture in R2A broth containing 0.1% tryptophan for 24 h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was 175 corresponding to that of IAA. To investigate the growth-promoting effects to the crops, the culture broth of Pantoea rwandensis 2SJ8-02 was infected to water cultures and seed pots of mung bean. In consequence, the adventitious root induction and root growth of mung bean were two times higher than those of the control.

충남 부여군 석성면 양송이 재배 농가에서 채취한 양송이 수확 후 배지로부터 auxin생성능이 뛰어난 세균 2SJ8-02 균주를 분리하여 TLC 및 HPLC 분석을 통해 확인한 결과, IAA 생산농도는 $122mg\;L^{-1}$이었으며 LC-Mass분석에 의하여 생성된 물질은 분자량이 175인 IAA로 확인되었다. 분리균에 의한 IAA 생산을 위한 최적조건을 실험한 결과, 0.1% L-tryptophan를 함유한 pH 7.0의 R2A broth배지에 $35^{\circ}C$, 24시간 배양 시 최대 이었다. 녹두발근 생검법과 pot 재배를 통한 식물생육효과 실험에서 분리균 배양액의 첨가는 대조구에 비해 발근수와 뿌리길이에서 약 2배의 뿌리신장효과를 보였다. 생리적 특성 및 계통학적 특성분석을 통해 분리균은 Gram 음성 간균인 Pantoea rwandensis 2SJ8-02로 동정되었다.

Keywords

References

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