Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Identification of Antiviral-related Genes Up-regulated in Response to Bombyx mori Nucleopolyhedrovirus

누에로부터 핵다각체병 바이러스 방어관련 유전자 정보 분석

  • Goo, Tae-Won (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Hong, Sun-Mee (Gyeongbuk Institute for Marine Bioindustry) ;
  • Kim, Sung-Wan (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Choi, Kwang-Ho (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Park, Seung-Won (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • 구태원 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 홍선미 (경북바이오산업연구소) ;
  • 김성완 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 최광호 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김성렬 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 박승원 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 강석우 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤은영 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2012.05.27
  • Accepted : 2012.10.08
  • Published : 2012.12.30
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Abstract

Silkworm larvae often suffer from viral infections causing heavy losses to the economy of silk industry. Insects exhibit both humoral and cellular immune responses that are effective against various pathohens like bacteria, fungi, protozoa, etc., but no insect immune responses is effective against viral infection. To obtain genes related to insect antiviral immunity from Bombyx mori, the cDNA library was constructed from B. mori nucleopolyhedrovirus (BmNPV)-infected B. mori. From the cDNA library, we selected 411 differentially expressed clones, and the 5' ends of the inserts were sequenced to generate ESTs. In this work, 135 unigenes were generated after the assembly of 411 differentially expressed clones ESTs. Of these 135 unigenes, we selected 109 antiviral response-related candidates except 26 clones that high similarity with genes derived from BmNPV. Among 109 unigenes, a total of 80% had significant matches to genes from other organisms in the database, wheres 20% of the unigenes had not matched in the database. Functional groups of these sequences with matches in database were constructed according to their putative biological function. Three largest categories were control of cellular oraganization (52%), metabolism (20%), and protein fate (10%). The genetic information reported in this study will provide more information about antiviral-related genes in silkworms.

누에 BmNPV는 잠사업에 있어서 가장 위해한 바이러스로써 익히 보고되었으며, 종종 잠사업의 심각한 경제적 손실을 야기하기도 한다. 곤충의 박테리아, 곰팡이 그리고 원생동물과 같은 다양한 병원체에 대응하는 곤충의 생체 방어기작에 대한 연구가 많이 알려져 있지만, 항바이러스 기작에 대한 연구는 매우 부족한 실정이다. 따라서 본 연구에서는 누에서 처음으로 누에의 BmNPV에 대한 생체방어 관련 유전자를 선발하기 위하여, 누에에 인위적으로 BmNPV를 주사하여 면역을 유도한 다음, 이로부터 cDNA 유전자은행을 제작하였다. 제작된 cDNA 유전자은행으로부터 무작위로 3,332개의 cDNA 클론을 선발하여 정상 누에에 비하여 BmNPV에 의해 면역이 유도된 누에에서 차별화 발현되는 109종의 잠정 항바이러스 유전자 클론을 차별화선별법에 의해서 분리하였다. 본 연구를 통해 확보한 109개의 유전자 정보는 누에의 바이러스에 대한 면역반응뿐만 아니라 최근에 개발된 누에 형질전환 기술을 이용하여 BmNPV 저항성 누에 품종을 개발하는데 중요한 기초 자료를 제공할 것으로 기대되며 또한, 인간의 중요한 항바이러스제 개발을 위한 모델 곤충으로써 누에를 이용하는데 기초 자료로 활용될 것으로도 기대된다.

Keywords

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