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Deregulated Expression of Cry1 and Cry2 in Human Gliomas

  • Luo, Yong (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Wang, Fan (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Chen, Lv-An (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Chen, Xiao-Wei (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Chen, Zhi-Jun (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Liu, Ping-Fei (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Li, Fen-Fen (Department of Neurosurgery, The First People's Hospital of Jingmen) ;
  • Li, Cai-Yan (Department of Neurosurgery, The Second People's Hospital of Jingmen) ;
  • Liang, Wu (Department of Neurosurgery, The First People's Hospital of Jingmen)
  • 발행 : 2012.11.30

초록

Growing evidence shows that deregulation of the circadian clock plays an important role in the development of malignant tumors, including gliomas. However, the molecular mechanisms of gene chnages controlling circadian rhythm in glioma cells have not been explored. Using real time polymerase chain reaction and immunohistochemistry techniques, we examined the expression of two important clock genes, cry1 and cry2, in 69 gliomas. In this study, out of 69 gliomas, 38 were cry1-positive, and 51 were cry2-positive. The expression levels of cry1 and cry2 in glioma cells were significantly different from the surrounding non-glioma cells (P<0.01). The difference in the expression rate of cry1 and cry 2 in high-grade (grade III and IV) and low-grade (grade 1 and II) gliomas was non-significant (P>0.05) but there was a difference in the intensity of immunoactivity for cry 2 between high-grade gliomas and low-grade gliomas (r=-0.384, P=0.021). In this study, we found that the expression of cry1 and cry2 in glioma cells was much lower than in the surrounding non-glioma cells. Therefore, we suggest that disturbances in cry1 and cry2 expression may result in the disruption of the control of normal circadian rhythm, thus benefiting the survival of glioma cells. Differential expression of circadian clock genes in glioma and non-glioma cells may provide a molecular basis for the chemotherapy of gliomas.

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참고문헌

  1. Bonnefont X, Albus H, Meijer JH, et al (2003). Light signalling in cryptochrome-deficient mice. Novartis Found Symp, 253, 56-109. https://doi.org/10.1002/0470090839.ch5
  2. Chen ST, Choo KB, Hou MF, et al (2005). Deregulated expression of the Per1, Per2, Per3 genes in breast cancers. Carcinogenesis, 26, 1241-46. https://doi.org/10.1093/carcin/bgi075
  3. Czeisler CA, Duffy JF, Shanahan TL, et al (1999). Stability, precision, and near-24-hour period of the human circadian pacemaker. Science, 284, 217-81.
  4. Delaunay F, Laudet V (2002). Circadian clock and microarrays: mammalian genome gets rhythm. Trends Genet, 18, 595-7. https://doi.org/10.1016/S0168-9525(02)02794-4
  5. Destici E, Oklejewicz M, Saito S, et al(2011). Mammalian cryptochromes impinge on cell cycle progression in a circadian clock-independent manner. Cell Cycle, 10, 3788-97. https://doi.org/10.4161/cc.10.21.17974
  6. Hanoun M, Eisele L, Suzuki M, et al (2012). Epigenetic silencing of the circadian clock gene CRY1 is associated with an indolent clinical course in chronic lymphocytic leukemia. PLoS One, 7, e34347. https://doi.org/10.1371/journal.pone.0034347
  7. Henrik O, Akira Y, Gijsbertus TJ (2002). Disruption of mCry2 restores circadian rhythmicity in mPer2 mutant mice. Genes Dev, 16, 2633. https://doi.org/10.1101/gad.233702
  8. Hua H, Wang Y, Wan C, et al (2007). Inhibition of tumorigenesis by intratumoral delivery of the circadian gene mPer2 in C57BL/6 mice. Cancer Gene Ther, 14, 815-8. https://doi.org/10.1038/sj.cgt.7701061
  9. Kei N, Kenta M, Masahiro K, et al (2005). The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice. Am J Physiol Regul Integr Comp Physiol, 288, R329-35. https://doi.org/10.1152/ajpregu.00395.2004
  10. Lee CC (2005) . The circadian clock and tumor suppression by Mammalian period genes. Methods Enzymol, 393, 852-61. https://doi.org/10.1016/S0076-6879(05)93045-0
  11. Lee S, Donehower LA, Herron AJ, et al (2010). Disrupting circadian homeostasis of sympathetic signaling promotes tumor development in mice. PLoS One, 5, e10995. https://doi.org/10.1371/journal.pone.0010995
  12. Li CY, Huang WF, Wang QL, et al (2012). Crocetin induces cytotoxicity in colon cancer cells via p53-independent mechanisms. Asian Pac J Cancer Prev, 13, 3757-61. https://doi.org/10.7314/APJCP.2012.13.8.3757
  13. Lowrey PL, Takahashi JS (2004). Mammalian circadian biology: elucidating genome-wide levels of temporal organization. Annu Rev Genomics Hum Genet, 5, 407-41. https://doi.org/10.1146/annurev.genom.5.061903.175925
  14. Matsuo T, Yamaguchi S, Mitsui S, et al (2003). Control of mechanism of the circadian clock for timing of cell division in vivo. Science, 302, 255-9. https://doi.org/10.1126/science.1086271
  15. Morse D, Sassone P (2002). Time after time: inputs to and outputs from the mammalian circadian oscillators. Trends Neurosci, 25, 632-7. https://doi.org/10.1016/S0166-2236(02)02274-9
  16. Panda S, Antoch MP, Miller BH et al (2002). Coordinated transcription of key pathways in the mouse by the circadian clock. Cell, 109, 307-20. https://doi.org/10.1016/S0092-8674(02)00722-5
  17. Reddy AB, Field MD, Maywood ES, et al (2002). Differential resynchronisation of circadian clock gene expression within the suprachiasmatic nuclei of mice subjected to experimental jet lag. Neurosci, 22, 7326-30.
  18. Reddy AB, Wong GK, O'Neill J, Maywood ES, Hastings MH (2005). Circadian clocks: neural and peripheral pacemakers that impact upon the cell division cycle. Mutat Res, 574, 76-91. https://doi.org/10.1016/j.mrfmmm.2005.01.024
  19. Reppert SM, Weaver DR (2001). Molecular analysis of mammalian circadian rhythms. Annu Rev Physiol, 63, 647-76. https://doi.org/10.1146/annurev.physiol.63.1.647
  20. Reppert SM, Weaver DR (2002). Coordination of circadian timing in mammals. Nature, 418, 935-41. https://doi.org/10.1038/nature00965
  21. Storch KF, Lipan O, Leykin I, et al (2002). Extensive and divergent circadian gene expression in liver and heart. Nature, 417, 78-83. https://doi.org/10.1038/nature744
  22. Vander HGT, Muijtjens M, Kobayashi K, et al (1999). Mammalian Cry1 and Cry2.are essential for maintenance of circadian rhythms. Nature, 398, 627-30. https://doi.org/10.1038/19323
  23. Yeh KT, Yang MY, Liu TC, et al (2005). Abnormal expression of Period 1 (Per1) in endometrial carcinoma. J Pathol, 206, 111-20. https://doi.org/10.1002/path.1756
  24. Young MW, Kay SA (2001). Time zones: a comparative genetics of circadian clocks. Nat Rev Genet, 2, 702-15. https://doi.org/10.1038/35088576

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