The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Expressed sequence tag analysis of Meretrix lusoria (Veneridae) in Korea

한국산 백합 (Meretrix lusoria) 의 전사체 분석

  • Kang, Jung-Ha (Biotechnology Research Division, NFRDI) ;
  • Jeong, Ji Eun (Department of Parasitology, College of Medicine and UHRC, Inje University) ;
  • Kim, Bong Seok (Biotechnology Research Division, NFRDI) ;
  • An, Chel-Min (Biotechnology Research Division, NFRDI) ;
  • Kang, Hyun-Sook (Biotechnology Research Division, NFRDI) ;
  • Kang, Se-Won (Department of Parasitology, College of Medicine and UHRC, Inje University) ;
  • Hwang, Hee Ju (Department of Parasitology, College of Medicine and UHRC, Inje University) ;
  • Han, Yeon Soo (Department of Agricultural Biology, Chonnam National University) ;
  • Chae, Sung-Hwa (Research Institute, GnC Bio Co., LTD.) ;
  • Ko, Hyun-Sook (Department of Biological Science, Silla University) ;
  • Lee, Jun-Sang (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Lee, Yong Seok (Department of Parasitology, College of Medicine and UHRC, Inje University)
  • 강정하 (국립수산과학원 생명공학부) ;
  • 정지은 (인제대학교 의과대학 기생충학교실 및 UHRC) ;
  • 김봉석 (국립수산과학원 생명공학부) ;
  • 안철민 (국립수산과학원 생명공학부) ;
  • 강현숙 (국립수산과학원 생명공학부) ;
  • 강세원 (인제대학교 의과대학 기생충학교실 및 UHRC) ;
  • 황희주 (인제대학교 의과대학 기생충학교실 및 UHRC) ;
  • 한연수 (전남대학교 농생명과학대학 농학과) ;
  • 채성화 ((주)지앤시바이오) ;
  • 고현숙 (신라대학교 생물과학과) ;
  • 이준상 (강원대학교 환경연구소) ;
  • 이용석 (인제대학교 의과대학 기생충학교실 및 UHRC)
  • Received : 2012.11.15
  • Accepted : 2012.12.19
  • Published : 2012.12.31
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Abstract

The importance of biological resources has been gradually increasing, and mollusks have been utilized as main fishery resources in terrestrial ecosystems. But little is known about genomic and transcriptional analysis in mollusks. This is the first report on the transcriptomic profile of Meretrix lusoria. In this study, we constructed cDNA library and determined 542 of distinct EST sequences composed of 284 singletons and 95 contigs. At first, we identified 180 of EST sequences that have significant hits on protein sequences of the exclusive Mollusks database through BLASTX program and 343 of EST sequences that have significant hits on NCBI NR database. We also found that 211 of putative sequences through local BLAST (blastx, E < e-10) search against KOG database were classified into 16 functional categories. Some kinds of immune response related genes encoding allograft inflammatory factor 1 (AIF-1), B-cell translocation gene 1 (BTG1), C-type lectin A, thioester-containing protein and 26S proteasome regulatory complex were identified. To determine phylogenetic relationship, we identified partial sequences of four genes (COX1, COX2, 12S rRNA and NADH dehydrogenase) that significantly matched with the mitochondrial genomes of 3 species-Ml (Meretrix lusoria), Mp (Meretrix petechialis) and Mm (Meretrix meretrix). As a result, we found that there was a little bit of a difference between sequences of Korean isolates and other known isolates. This study will be useful to develop breeding technology and might also be helpful to establish a classification system.

Keywords

References

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