한국패류학회지 (The Korean Journal of Malacology)

한국패류학회 (The Malacological Society of Korea)
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나프탈렌에 노출된 바지락, Ruditapes philippinarum의 생존 및 생식지표 변화

Change of Survival and Reproductive Indicator of the Manila Clam, Ruditapes philippinarum Following Chronic Exposure to Naphthalene

  • 진영국 (국립수산과학원 남서해수산연구소) ;
  • 김태익 (국립수산과학원 남서해수산연구소) ;
  • 명정인 (국립수산과학원 남서해수산연구소) ;
  • 황형규 (국립수산과학원 남서해수산연구소) ;
  • 박민우 (국립수산과학원 남서해수산연구소)
  • 투고 : 2012.11.06
  • 심사 : 2012.11.28
  • 발행 : 2012.12.31
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초록

나프탈렌이 바지락 (각장 $32.74{\pm}2.18mm$, 전중 $8.29{\pm}1.41g$) 의 생존, 성비 및 생식소발달에 미치는 영향에 대해 연구하였다. 노출농도는 대조구, solvent 대조구 (에탄올), 30, 60, 90, $120{\mu}g$ Nap $L^{-1}$ 였으며, 노출기간은 20주였다. 노출 종료 후 바지락의 생존율은 $60{\mu}g$ Nap $L^{-1}$ 이상의 농도에서 감소하였다. 성비는 대조구와 비교해 solvent 대조구 (에탄올)와 $30{\mu}g$ Nap $L^{-1}$ 실험구는 별다른 차이를 보이지 않았지만, $60{\mu}g$ Nap $L^{-1}$ 실험구의 경우 수컷의 비율이 높았다. 그 후 농도가 높아질수록 수컷의 비율이 감소하였다. 생식소 발달단계는 암컷의 경우 $60{\mu}g$ Nap $L^{-1}$ 이상의 농도에서, 수컷의 경우 $30{\mu}g$ Nap $L^{-1}$ 이상의 농도에서 발달이 지연되는 결과를 보였다. 생식소에서 관찰된 intersex는 암컷에서 16.28%, 수컷에서 1.68%를 나타냈다. 이러한 결과들을 볼 때 나프탈렌은 바지락의 생존 뿐 만 아니라 성비와 생식에 영향을 주는 것으로 보인다.

Manila clam, Ruditapes philippinarum (SL; $32.74{\pm}2.18mm$, TW; $8.29{\pm}1.41g$, N; 450) were exposed to various concentrations of naphthalene for 20 weeks. Exposure concentrations of naphthalene established control, solvent control (ethanol), 30, 60, 90 and $120{\mu}g$ Nap $L^{-1}$ following results of 96h acute exposure. After exposure during 20 weeks, survival rate of the manila clam was lowed in 60, 90 and $120{\mu}g$ Nap $L^{-1}$ exposure group compared control group. Also, sex ratio of male was higher in $60{\mu}g$ Nap $L^{-1}$ exposure group (${\chi}^2=5.492$, P < 0.05) but lowed $90{\mu}g$ Nap $L^{-1}$ exposure group (${\chi}^2=4.214$, P < 0.05) and $120{\mu}g$ Nap $L^{-1}$ exposure group (${\chi}^2=30.118$, P < 0.05). Gonad development was delayed in female (> $60{\mu}g$ Nap $L^{-1}$) and male (> $30{\mu}g$ Nap $L^{-1}$). Intersex was 16.28% in female, 1.68% in male. In this result, naphthalene caused survival decrease, imbalance of sex ratio, delay of gonad development of the manila clam. Also, chronic exposure to naphthalene suggested abnormal effects in reproduction of the manila clam.

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