미생물학회지 (Korean Journal of Microbiology)

  • 제48권4호
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  • Pages.309-313
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  • 2012
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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Multiplex PCR 분석을 통한 건강한 20대 여성의 질 내에 서식하는 Lactobacillus 속 유산균의 신속한 검출

Rapid Detection of Lactobacillus Genus Inhabiting in Vagina of 20's Healthy Women Using Multiplex PCR

  • 오초롱 (서경대학교 화학생명공학과) ;
  • 조홍범 (서경대학교 화학생명공학과)
  • Oh, Cho-Rong (Department of Chemical and Biotechnology, Seokyeong University) ;
  • Cho, Hong-Bum (Department of Chemical and Biotechnology, Seokyeong University)
  • 투고 : 2012.09.26
  • 심사 : 2012.10.12
  • 발행 : 2012.12.31
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초록

본 연구는 multiplex PCR 방법을 적용하여 질 내부에 서식하는 유산균을 동시에 그리고 신속하게 검출하는 방법을 개발하는 것을 목적으로 수행되었다. 세균의 감염에 의한 질염 증상이 없는 건강한 20대 여성 166명으로부터 질 분비물을 채취하였다. 건강한 여성의 질 내부에 서식하는 것으로 확인된 Lactobacillus 속에 속하는 6종의 유산균을 종 특이 multiplex PCR primer를 제작하기 위해 선정하였다. Multiplex primer I은 L. iners, L. crispatus, L. gasseri를 선택적으로 검출하기 위해 특성화하였고 multiplex primer II는 L. acidophilus, L. jensenii, L. fermentum를 선택적으로 검출하기 위해 특성화하였다. Multiplex PCR 기술을 이용하여 분석한 결과 L. crispatus (77%)가 가장 높은 빈도로 검출되었고 L. acidophilus (57%)과 L. jensenii (57%)는 상대적으로 높은 빈도로 검출되었다. L. iners (59%)는 L. acidophilus (57%)와 L. jensenii (57%) 보다 높은 빈도로 검출되었지만 항생제 치료 과정 후 또는 세균성 질염의 경우에도 발견되는 균종으로 건강한 여성에게서 주로 서식하는 종이라고 결론 내리기는 어렵다. 결론적으로, 특정 유산균의 특이적인 primer를 이용한 multiplex PCR 기술은 질의 건강상태의 변화 또는 질의 질병으로부터 회복 과정을 예측할 수 있는 도구로서 유용할 것으로 생각된다.

The purpose of this study is to simultaneously detect and identify specific genus of Lactobacillus distributed in vagina of healthy women in their twenties by using multiplex primer. Vaginal fluid samples were taken from 166 women who were healthy and did not have vaginosis symptoms caused by bacterial infection. Six species of lactic acid bacteria belong to Lactobacillus genus that are known to inhabit in healthy vagina were selected to make a species-specific multiplex primer. Multiplex primer I was specified to selectively detect L. iners, L. crispatus, L. gasseri and multiplex primer II was specified to selectively detect L. acidophilus, L. jensenii, L. fermentum. L. crispatus (77%) was most frequently detected, and L. acidophilus (57%) and L. jensenii (57%) were relatively higher than others. Although the proportion of L. iners (59%) was a little higher than those of L. acidophilus and L. jensenii, this should be further validated in healthy women's vagina to be sure since they often appear in women having bacterial vaginosis and/or during the antibiotic therapy. Conclusively, the multiplex PCR technique using the species-specific primer could a useful tool to predict variation of vaginal health condition and process of recovery from vaginal disease.

키워드

참고문헌

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피인용 문헌

  1. Probiotics in the Prevention and Treatment of Postmenopausal Vaginal Infections: Review Article vol.23, pp.3, 2017, https://doi.org/10.6118/jmm.2017.23.3.139
  2. 대황으로부터 세균성 질염 치료를 위한 항균성 물질의 분리 및 특성 vol.32, pp.2, 2012, https://doi.org/10.7841/ksbbj.2017.32.2.133
  3. Forensic Microbiological Analysis for Vaginal Fluid Identification Using Multiplex Real-Time PCR System vol.45, pp.1, 2021, https://doi.org/10.7580/kjlm.2021.45.1.14