Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지
DOI QR코드

DOI QR Code

Antifungal and Antioxidant Activities of Volatile Organic Compounds Generated During the Drying Process of Chamaecyparis obtuse

편백나무 건조 중 발생하는 휘발성 유기화합물의 항진균 및 항산화 활성

  • Seo, Young-Jun (Department of Forest Products and Technology (BK21 Program), Chonnam National University) ;
  • Lee, Jae-Won (Department of Forest Products and Technology (BK21 Program), Chonnam National University)
  • 서영준 (전남대학교 농업생명과학대학 산림자원학부) ;
  • 이재원 (전남대학교 농업생명과학대학 산림자원학부)
  • Received : 2012.07.06
  • Accepted : 2012.09.28
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we evaluated antifungal and antioxidant activities of condensed liquid of volatile organic compounds generated during the drying process of Chamaecyparis obtuse. Condensed liquid of volatile organic compounds were extracted with hexane and ethyl acetate, respectively. The extracts were used for antifungal activity at range from 100 to 500 mg/L Antifungal activity of extracts increased as the extracts concentration increased, the activity against tested dermatophytes was high at 500 mg/L. Among extract solvents, antifungal activity was higher at hexane extract than the ethyl acetate. Especially, the antifungal activity against Trichophyton rubrum was higher than other tested dermatophytes. Antioxidant activity was 90% above 25 mg/L regardless of the extractive solvents, while the hexane extract showed high antioxidant activity below 25 mg/L. Major compounds of hexane extract were torreyol, alpha-cadinol, and tau-cadinol. However, major compounds of ethyl acetate extract were alpha-amorphene, alpha-cadinol, and gamma-cadinene.

본 연구는 편백나무 건조 중 발생하는 휘발성 유기화합물로 구성된 응축수에 대해 항진균 및 항산화 활성을 평가하였다. 응축수는 hexane과 ethyl acetate로 각각 추출하였으며 추출액의 농도 100-500 mg/L에서 피부사상균에 대한 항진균 활성을 측정하였다. 추출액 농도가 증가할수록 항진균 효과도 증가하였으며 500 mg/L에서는 모든 피부사상균에 대해 높은 항진균 활성을 나타냈다. 추출용매에 따라서는 hexane으로 추출하였을 경우 높은 활성을 나타냈으며, 특히 Trichophyton rubrum에 대한 항진균 활성이 높았다. 항산화 활성을 측정한 결과 25 mg/L 이상의 농도에서는 90% 이상의 항산화 활성을 나타냈으며 추출용매에 따른 차이는 거의 없었다. 하지만 25 mg/L 이하의 농도에서는 hexane 추출물에서 높은 항산화 활성을 나타냈다. 각각의 추출물 구성성분을 분석한 결과 hexane 추출물의 주요 구성성분은 torreyol, alpha-cadinol, tau-cadinol이며, ethyl acetate 추출물의 주요 구성성분은 alpha-amorphene, alpha-cadinol, gamma-cadinene으로 나타났다.

Keywords

References

  1. Brand-Williams, W., Cuvelier, M.E., and Berset, C. 1995. Use of a free radical method to evaluate antioxidant acitivity. LWT-Food Sci. Technol. 28, 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  2. Cronn, D., Truitt, S., and Chmpbell, M. 1983. Chemical characterization of plywood veneer dryer emission. Atmospheric Environment 17, 201 -211. https://doi.org/10.1016/0004-6981(83)90034-3
  3. Englund, F. and Nussbaum, R. 2000. Monoterpenes in Scots pine and Norway spruce and their emission during kiln drying. Holzforschung 54, 449-456.
  4. Gwak, K.S., Park, M.J., Jeung, E.B., Chang, J.W., and Choi, I.G. 2006. Comparison of antifungal activities of monoterpenes and sesquiterpenes in essential oil from Chamaecyparis obtuse against dermatophytes. Mokchae Konghak 34, 46-55.
  5. Haznedaroglu, M., Karabay, N., and Zeybek, U. 2001. Antibacterial activity of Salvia tomentosa essential oil. Fitoterapia 72, 829-831. https://doi.org/10.1016/S0367-326X(01)00335-5
  6. Kim, H.K., Kim, B.C., Shin, C.G., Jeong, S.I., Kim, H.J., and Ju, Y.S. 2004. Susceptibility of oral bacteria to essential oil of Artemisia capillaris Thunb. Korean J. Oriental Medicine 25, 121-128.
  7. Kim, S.H., Lee, S.Y., Hong, C.Y., Gwak, K.S., Yeo, H.M., Lee, J.J., and Choi, I.G. 2011. Whitening and antioxidant activities of essential oils from CryPtomeria japonica and Chamaecyparis obtuse. Mokchae Konghak 39, 291-302.
  8. Lee, S.Y., Gwak, K.S., Kim, S.H., Lee, J.J., Yeo, H.M., and Choi, I.G. 2010. Analysis of terpenoids as volatile compound released during the drying process of Cryptomeria japonica. Mokchae Konghak 38, 242-250. https://doi.org/10.5658/WOOD.2010.38.3.242
  9. McDonald, A., Dare, P., Gifford, J., Steward, D., and Riley, S. 2002. Assessment of air emissions from industrial kiln drying of Pinus radiate wood. Holz als Roh-und Werkstoff 60, 181-190. https://doi.org/10.1007/s00107-002-0293-1
  10. McDonald, A. and Wastney, S. 1995. Analysis of volatile emissions from kiln drying of radiate pine. Vol. 3, pp. 431-436. In proc 8th Int. symp. of wood pulping chemistry. Helsinki, Finland.
  11. McGarvey, D. and Croteau, R. 1995. Terpenoid metabolism. Plant Cell. 7, 1015. https://doi.org/10.1105/tpc.7.7.1015
  12. Park, M.J., Choi, W.S., Min, B.C., Kim, H.Y., Kang, H.Y., and Choi, I.G. 2008. Antioxidant activities of essential oils from Chamaecyparis obtusa. Mokchae Konghak. 36, 159-167. https://doi.org/10.5658/WOOD.2008.36.6.159
  13. Park, M.J., Lee, S.M., Gwak, K.S., Jeung, E.B., Chang, W.J., and Choi, I.G. 2005. Investigation of active antifungal compounds of essential oil from Chamaecyparis obstusa against dermatophytes, Microsporum canis and Trichophyton Mentagrophytes. Mokchae Konghak 33, 72-78.
  14. Sode, K., Karube, I., Araki, R., and Mikami, Y. 1989. Microbial conversion of ${\beta}$-ionone by immobilized Aspergillus niger in the presence of an organic solvent. Biotechnol. Bioeng. 33, 1191-1195. https://doi.org/10.1002/bit.260330915
  15. Soliman, F.M., EI-Kashooury, E.A., Dathy, M., and Gonaid, M.H. 1994. Analysis and biological activity of the essential oil of Rosamarinus officinalis L. from Egyptian. J. Flavour Fragrance 9, 29-33. https://doi.org/10.1002/ffj.2730090107
  16. Yaeno, A., Nakai, Y., Hayakawa, R., and Nishino, T. 2003. Antibacterial effect of beta-thujaplicin on Staphylococci isolated from atopic dermatitis. J. Antimicrob. Chemother. 51, 113-122. https://doi.org/10.1093/jac/dkg037
  17. Vila, J., Angel, F., and Limona, X. 1999. Volvariella nigrovolvacea kosina var. Catalana Micol. 22, 131-135.

Cited by

  1. Emission Characteristics of Volatile Organic Compounds by Humidifier with Using Hinoki Cypress Extracts vol.42, pp.6, 2014, https://doi.org/10.5658/WOOD.2014.42.6.747