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Physicochemical Properties of Barley β-Glucan with Different Heating Temperatures

열처리 온도에 따른 보리 β-Glucan의 이화학적 특성

  • Lee, Sang Hoon (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jang, Gwi Yeong (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Hyun Young (Dept. of Functional Crop, National Institute of Crop Science) ;
  • Woo, Koan Sik (Dept. of Functional Crop, National Institute of Crop Science) ;
  • Hwang, In Guk (Dept. of Agrofood Resources, National Academy of Agricultural Science) ;
  • Kim, Kee Jong (Division of Rice and Winter Cereal Crop, National Institute of Crop Science) ;
  • Lee, Mi Ja (Division of Rice and Winter Cereal Crop, National Institute of Crop Science) ;
  • Kim, Tae Jip (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Junsoo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 이상훈 (충북대학교 식품공학과) ;
  • 장귀영 (충북대학교 식품공학과) ;
  • 김현영 (국립식량과학원 기능성작물부) ;
  • 우관식 (국립식량과학원 기능성작물부) ;
  • 황인국 (국립식량과학원 농식품자원부) ;
  • 김기종 (국립식량과학원 벼맥류부) ;
  • 이미자 (국립식량과학원 벼맥류부) ;
  • 김태집 (충북대학교 식품공학과) ;
  • 이준수 (충북대학교 식품공학과) ;
  • 정헌상 (충북대학교 식품공학과)
  • Received : 2012.08.03
  • Accepted : 2012.09.03
  • Published : 2012.12.31

Abstract

This study was performed to investigate the changes of total and soluble ${\beta}$-glucan contents, purity, and physical characteristics of three heated barley varieties: Saessalbori (SSB), Saechalssalbori (SCSB), and Hinchalssalbori (HCSB). The barleys were heated at different temperatures of 110, 120, 130, 140 and $150^{\circ}C$ for 2 hours. The total ${\beta}$-glucan contents of raw SSB, SCSB, and HCSB were 8.40, 7.77 and 8.28%, and the soluble ${\beta}$-glucan contents were 4.79, 4.14, and 4.61%, respectively. After heating at $130^{\circ}C$, the total ${\beta}$-glucan contents increased to 11.59, 14.6, and 13.36%, as did the soluble ${\beta}$-glucan contents to 4.21, 7.96, and 7.23%, respectively. The purities of soluble ${\beta}$-glucan of the raw barleys were 35.11, 32.74 and 25.62%, but after heating at $150^{\circ}C$, it increased to 83.43, 91.02, and 88.01%, respectively. The molecular weight and viscosity of the ${\beta}$-glucan solution decreased with increasing heating temperature. The re-solubility of raw barley ${\beta}$-glucan was about 50%, but it was increased to 97% with increasing heating temperature. These results suggest that heating of ${\beta}$-glucan can improve the utilization of barley ${\beta}$-glucan.

열처리에 따른 보리 ${\beta}$-glucan의 이화학적 특성변화를 살펴보기 위하여 새쌀보리, 새찰쌀보리 및 흰찰쌀보리를 $110^{\circ}C{\sim}150^{\circ}C$에서 2시간 열처리하였으며, 총 및 수용성 ${\beta}$-glucan 함량, 순도, 분자량, 점도 및 재용해율을 살펴보았다. 3품종 보리의 총 ${\beta}$-glucan 함량은 7.77~8.40% 범위였으며, 열처리 온도가 증가함에 따라 11.59~14.65% 범위로 증가하였으나, $140^{\circ}C$$150^{\circ}C$에서는 6.30~8.33% 범위로 감소하였다. 수용성 ${\beta}$-glucan의 함량은 무처리의 4.14~4.79% 범위에서 열처리 온도가 증가함에 따라 6.11~10.29%까지 증가하였으며, $150^{\circ}C$에서는 3.01~5.60% 범위로 감소하였다. 수용성 ${\beta}$-glucan의 순도는 3품종 모두 $130^{\circ}C$까지는 31~37%범위이었으나 $140^{\circ}C$ 이상에서는 91%까지 증가하였다. 분자량은 메성보리보다 찰성보리가 더 컸으며, 열처리 온도가 증가함에 따라 새쌀보리는 606,463 Da에서 1,404 Da으로, 새찰쌀보리는 698,541 Da에서 1,617 Da으로, 그리고 흰찰쌀보리는 669,539 Da에서 1,550 Da으로 감소하였다. ${\beta}$-Glucan 수용액의 점도는 열처리 온도가 증가함에 따라 감소하였으며, 메성보리보다 찰성보리가 높았고 흰찰쌀보리보다 새찰쌀보리가 높았다. 재용해율은 무처리의 50~55% 범위에서 열처리 온도가 증가함에 따라 증가하여 $150^{\circ}C$에서는 96.75~97.99% 범위로 증가하였다.

Keywords

References

  1. Aman P, Graham H. 1987. Analysis of total and insouble mixed-linked (1${\rightarrow}$3), (1${\rightarrow}$4)-${\beta}$-glucans in barley and oats. J Agric Food Chem 35: 704-709. https://doi.org/10.1021/jf00077a016
  2. Hockett EA, McGuire CF, Newman CW, Prentice N. 1987. The relationship of barley ${\beta}$-glucan content to agronomic and quality characteristics. Barley Genetics V: 851-860.
  3. Lee YT, Lee CK. 1994. Effects of varietal variation in barley on ${\beta}$-glucan and malting quality characteristics. Korean J Food Sci Technol 26: 172-177.
  4. McCleary BV, Glennie-Holmes M. 1985. Enzymatic quantification of (1${\rightarrow}$3)(1${\rightarrow}$4)-${\beta}$-D-glucan in barley and malt. J Inst Brew 91: 285-295. https://doi.org/10.1002/j.2050-0416.1985.tb04345.x
  5. Bourne DT, Wheeler RE. 1984. Environmental and varietal differences in total ${\beta}$-glucan contents of barley and the effectiveness of its breakdown under different malting conditions. J Inst Brew 90: 306-310. https://doi.org/10.1002/j.2050-0416.1984.tb04280.x
  6. Henry RJ. 1986. Genetic and environmental variation in the pentosan and ${\beta}$-glucan contents of barley, and their relation to malting quality. J Cereal Sci 4: 269-277. https://doi.org/10.1016/S0733-5210(86)80029-7
  7. Buliga GS, Brant DA, Fincher GB. 1986. The sequence statistics and solution conformation of a barley (1${\rightarrow}$3), (1${\rightarrow}$4)-${\beta}$-D-glucan. Carbohydr Res 157: 139-156. https://doi.org/10.1016/0008-6215(86)85065-0
  8. Woodward JR, Fincher GB, Stone BA. 1983. Water-soluble (1${\rightarrow}$3), (1${\rightarrow}$4)-${\beta}$-D-glucans from barley (Hordeum vulgare) endosperm. II. Fine structure. Carbohydr Polym 3: 207-225. https://doi.org/10.1016/0144-8617(83)90019-X
  9. Lee YT. 1996. ${\beta}$-Glucans in barley and oats and their changes in solubility by processing. Agric Chem Biotechnol 39: 482-487.
  10. Klopfenstein CF. 1988. The role of cereal ${\beta}$-glucans in nutrition and health. Cereal Foods World 33: 865-866.
  11. Bae IY, Lee SY, Kim SM, Lee HG. 2009. Effect of partially hydrolyzed oat ${\beta}$-glucan on the weight gain and lipid profile of mice. Food Hydrocolloids 23: 2016-2021. https://doi.org/10.1016/j.foodhyd.2009.03.016
  12. Bamforth CW. 1985. Biochemical approaches to beer quality. J Inst Brew 91: 154-160. https://doi.org/10.1002/j.2050-0416.1985.tb04322.x
  13. Campbell GL, Bedford MR. 1992. Enzyme applications for monogastric feeds: a review. Can J Anim Sci 72: 449-466. https://doi.org/10.4141/cjas92-058
  14. Carr JM, Glatter S, Jeraci JL, Lewis BA. 1990. Enzymic determination of ${\beta}$-glucan in cereal-based food products. Cereal Chem 67: 226-229.
  15. Hasegawa M, Isogai A, Onabe F. 1993. Preparation of lowmolecular- weight chitosan using phosphoric acid. Carbohydr Polym 20: 279-283. https://doi.org/10.1016/0144-8617(93)90100-I
  16. Roubroeks JP, Andersson R, Mastromauro DI, Christensen BE, Aman P. 2001. Molecular weight, structure and shape of oat (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-D-glucan fractions obtained by enzymatic degradation with (1${\rightarrow}$4)-${\beta}$-D-glucan-4-glucanohydrolase from Trichoderma reesi. Carbohydr Polym 46: 275-285. https://doi.org/10.1016/S0144-8617(00)00329-5
  17. Machova E, Kogan G, Chorvatovicová D, Sandula J. 1999. Ultrasonic depolymerization of the chitin-glucan complex from Aspergillus niger and antimutagenic activity of its product. Ultrason Sonochem 6: 111-114. https://doi.org/10.1016/S1350-4177(98)00024-8
  18. Byun EH, Kim JH, Sung NY, Choi JI, Lim ST, Kim KH, Yool HS, Byun MW, Lee JW. 2008. Effects of gamma irradiation on the physical and structural properties of ${\beta}$-glucan. Radiat Phys Chem 77: 781-786. https://doi.org/10.1016/j.radphyschem.2007.12.008
  19. Jeon YI, Kim SK. 2002. Production of chitooligosaccharides using an ultrafiltration membrane reactor and their antibacterial activity. Carbohydr Polym 41: 133-141.
  20. Brandt LM, Jeltema MA, Zabik ME, Jeltema BD. 1984. Effects of cooking in solutions of varying pH on the dietary fiber components of vegetables. J Food Sci 49: 900-904. https://doi.org/10.1111/j.1365-2621.1984.tb13237.x
  21. Hwang JK, Kim CT, Hong SI, Kim CJ. 1994. Solubilization of plant cell walls by extrusion. J Korean Soc Food Nutr 23: 358-370.
  22. Ralet MC, Della Valle G, Thibault JF. 1993. Raw and extruded fibre from pea hulls. Part I: Composition and physico-chemical properties. Carbohydr Polym 20: 17-23. https://doi.org/10.1016/0144-8617(93)90028-3
  23. Ralet MC, Saulnier L, Thibault JF. 1993. Raw and extruded fibre from pea hulls. Part II: Structural study of the water- soluble polysaccharides. Carbohydr Polym 20: 25-34. https://doi.org/10.1016/0144-8617(93)90029-4
  24. Ralet MC, Thibault JF, Della Valle G. 1991. Solubilization of sugar-beet pulp cell wall polysaccharides by extrusion-cooking. Lebensm-Wiss Technol 24: 107-112.
  25. Guillon F, Barry JL, Thibault JF. 1992. Effect of autoclaving sugar-beet fibre on its physico-chemical properties and its in-vitro degradation by human faecal bacteria. J Sci Food Agric 60: 69-79. https://doi.org/10.1002/jsfa.2740600112
  26. Hwang IG, Woo KS, Jeong HS. 2011. Biological activity and heat treatment processing of foods. Food Sci Ind 44: 56-65.
  27. Wood PJ, Paton D, Siddique IR. 1977. Determination of ${\beta}$-glucan in oats and barley. Cereal Chem 54: 524-533.
  28. Kim SY, Ryu CH. 2003. Extraction and physicochemical characterization of barley bran ${\beta}$-glucan. Korean J Soc Food Cookery Sci 19: 616-623.
  29. Jeong HS, Kang TS, Park HJ, Jung IS, Lee HY. 2004. Characteristics of viscosity and components of soluble extract in oats. Food Eng Prog 8: 40-46.
  30. Marlett JA. 1991. Dietary fiber content and effect of processing on two barley varieties. Cereal Foods World 36: 576-578.
  31. Forrest IS, Wainwright T. 1977. The mode of binding of ${\beta}$-glucans extracted from barley at different temperatures. Carbohydr Res 83: 279-286.
  32. Kim SR, Choi HD, Seog HM, Kim SS, Lee YT. 1999. Physicochemical characteristics of ${\beta}$-glucan isolated from barley. Korean J Food Sci Technol 31: 1164-1170.
  33. Gomeza C, Navarro A, Manzanares P, Horta A, Carbonell JV. 1997. Physical and structural properties of barley (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-D-glucan. Part I. Determination of molecular weight and macromolecular radius by light scattering. Carbohydr Polym 32: 7-15. https://doi.org/10.1016/S0144-8617(96)00126-9
  34. Choi HD, Seog H, Choi I, Park Y, Lee C, Shin K. 2004. Molecular structure of ${\beta}$-glucan isolated from non-waxy and waxy barley. Food Sci Biotechnol 13: 744-748.
  35. Kivela R, Sontag-Strohm T, Loponen J, Tuomainen P, Nystrom L. 2011. Oxidative and radical mediated cleavage of ${\beta}$-glucan in thermal treatment. Carbohydr Polym 85: 645-652. https://doi.org/10.1016/j.carbpol.2011.03.026
  36. Bradley TD, Ball A, Harding SE, Mitchell JR. 1989. Thermal degradation of guar gum. Carbohydr Polym 10: 205-214. https://doi.org/10.1016/0144-8617(89)90012-X
  37. Ullrich SE, Clancy JA, Eslick RF, Lance RCM. 1986. ${\beta}$-Glucan content and viscosity of extracts from waxy barley. J Cereal Sci 4: 279-285. https://doi.org/10.1016/S0733-5210(86)80030-3
  38. Varum KM, Simidsrod O. 1988. Partial chemical and physical characterisation of (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-glucans from oat (Avena sativa L.) aleurone. Carbohydr Polym 9: 103-117. https://doi.org/10.1016/0144-8617(88)90008-2
  39. Beer MU, Arrigoni E, Amado R. 1996. Extraction of oat gum from oat bran: effects of process on yield, molecular weight distribution, viscosity and (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-D-glucans content of the gum. Cereal Chem 73: 58-62.

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